Freshwater phage uvFW-CGR-AMD-COM-C493

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0XUJ2|A0A1B0XUJ2_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C493 OX=1838157 GN=uvFWCGRAMDCOMC493_06 PE=4 SV=1
MM1 pKa = 7.24IAQYY5 pKa = 9.78FAQINEE11 pKa = 4.12QNMVTDD17 pKa = 3.77IAVVHH22 pKa = 6.47RR23 pKa = 11.84EE24 pKa = 3.87FLEE27 pKa = 4.37ANPDD31 pKa = 3.46RR32 pKa = 11.84YY33 pKa = 9.61QGRR36 pKa = 11.84WVEE39 pKa = 4.1TFYY42 pKa = 10.67DD43 pKa = 4.2TEE45 pKa = 4.16GKK47 pKa = 8.36TYY49 pKa = 10.92AGIGMIYY56 pKa = 10.61DD57 pKa = 3.56EE58 pKa = 4.54TTKK61 pKa = 10.87NFYY64 pKa = 10.22IPYY67 pKa = 8.28TPPEE71 pKa = 4.03EE72 pKa = 4.15

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0XUH1|A0A1B0XUH1_9CAUD Uncharacterized protein OS=Freshwater phage uvFW-CGR-AMD-COM-C493 OX=1838157 GN=uvFWCGRAMDCOMC493_05 PE=4 SV=1
MM1 pKa = 7.42AVRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 9.29INYY10 pKa = 7.12MGSLAEE16 pKa = 4.81FKK18 pKa = 10.93QSVNDD23 pKa = 3.77SQVEE27 pKa = 4.3GGGGAASIMGGGVMPGSFGGGTRR50 pKa = 11.84DD51 pKa = 3.07TITINEE57 pKa = 4.25EE58 pKa = 3.56GGTDD62 pKa = 3.32DD63 pKa = 4.17RR64 pKa = 11.84GYY66 pKa = 11.53YY67 pKa = 10.19DD68 pKa = 5.42FPDD71 pKa = 4.62PYY73 pKa = 10.81KK74 pKa = 10.6PNPRR78 pKa = 11.84RR79 pKa = 11.84NRR81 pKa = 11.84PDD83 pKa = 3.21QSFKK87 pKa = 10.63PADD90 pKa = 4.07RR91 pKa = 11.84YY92 pKa = 11.08GDD94 pKa = 4.05PPPPGQMPPRR104 pKa = 11.84PVDD107 pKa = 4.04RR108 pKa = 11.84YY109 pKa = 10.63PEE111 pKa = 4.22ATPNRR116 pKa = 11.84PTRR119 pKa = 11.84PRR121 pKa = 11.84KK122 pKa = 9.31PKK124 pKa = 9.2PKK126 pKa = 9.61KK127 pKa = 9.23PKK129 pKa = 9.91GRR131 pKa = 11.84GAGGSTTQGAMM142 pKa = 3.51

Molecular weight: 15.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

10749

34

1690

238.9

26.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.703 ± 0.549

0.4 ± 0.093

5.377 ± 0.342

6.261 ± 0.518

3.107 ± 0.224

8.643 ± 0.805

1.116 ± 0.198

4.81 ± 0.24

6.41 ± 0.686

7.582 ± 0.503

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.205

4.168 ± 0.218

4.977 ± 0.403

4.382 ± 0.268

5.787 ± 0.378

6.587 ± 0.293

7.452 ± 0.764

6.512 ± 0.271

1.247 ± 0.165

3.144 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski