Aspergillus steynii IBT 23096

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Circumdati; Aspergillus steynii

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12965 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I2G879|A0A2I2G879_9EURO Uncharacterized protein (Fragment) OS=Aspergillus steynii IBT 23096 OX=1392250 GN=P170DRAFT_358376 PE=4 SV=1
MM1 pKa = 7.49SPAPLTTLLAAAASAPLLFSTGVSAQNATSRR32 pKa = 11.84AQIAMDD38 pKa = 5.0VLQTWYY44 pKa = 10.83NGTSGIWDD52 pKa = 3.47TCGWWNGANCLTTLADD68 pKa = 3.69FAIADD73 pKa = 3.93EE74 pKa = 4.58SVNDD78 pKa = 3.77AVVGVLGTTFKK89 pKa = 11.02VATISNPIPEE99 pKa = 4.2RR100 pKa = 11.84SGDD103 pKa = 3.41EE104 pKa = 4.43FYY106 pKa = 11.22NITDD110 pKa = 3.85GTQAGSPDD118 pKa = 3.47AYY120 pKa = 10.67QWMDD124 pKa = 2.75GSYY127 pKa = 11.38DD128 pKa = 3.82DD129 pKa = 4.97DD130 pKa = 4.1AWWALAWIAAYY141 pKa = 10.15DD142 pKa = 3.71VTDD145 pKa = 4.22DD146 pKa = 6.04DD147 pKa = 5.22EE148 pKa = 5.06YY149 pKa = 11.86LDD151 pKa = 3.94LAIGIFDD158 pKa = 4.27RR159 pKa = 11.84LSHH162 pKa = 6.03TWPSKK167 pKa = 10.13CGNGGIDD174 pKa = 3.06SDD176 pKa = 4.34YY177 pKa = 10.98SHH179 pKa = 7.52VYY181 pKa = 9.65VNAVTNEE188 pKa = 3.93LFFSVAAHH196 pKa = 6.54LANRR200 pKa = 11.84ASDD203 pKa = 3.39SQYY206 pKa = 9.88YY207 pKa = 9.52VDD209 pKa = 3.33WARR212 pKa = 11.84RR213 pKa = 11.84QWNWFNATGMINEE226 pKa = 4.69NYY228 pKa = 8.39TVNDD232 pKa = 4.04GLTNDD237 pKa = 4.41CANNGDD243 pKa = 4.43TIWTYY248 pKa = 10.49NQGVVIGGLAEE259 pKa = 3.95LHH261 pKa = 6.2RR262 pKa = 11.84SVSNSSNSSYY272 pKa = 11.4LDD274 pKa = 3.35MAEE277 pKa = 4.88KK278 pKa = 9.89IAKK281 pKa = 10.16ASIDD285 pKa = 3.6EE286 pKa = 4.5LADD289 pKa = 3.71DD290 pKa = 4.35NYY292 pKa = 10.75VIRR295 pKa = 11.84EE296 pKa = 4.17SCEE299 pKa = 3.7PDD301 pKa = 3.11CDD303 pKa = 4.57ANATQFKK310 pKa = 10.81GIFIRR315 pKa = 11.84NLKK318 pKa = 9.37LLHH321 pKa = 6.55SVAPDD326 pKa = 3.36DD327 pKa = 4.43VYY329 pKa = 11.64EE330 pKa = 4.13KK331 pKa = 10.77VIRR334 pKa = 11.84ACADD338 pKa = 3.88SIWDD342 pKa = 3.69NDD344 pKa = 3.76RR345 pKa = 11.84DD346 pKa = 3.8EE347 pKa = 4.49QNQFGINWAGPVSAIDD363 pKa = 3.74ASTHH367 pKa = 5.38SSAMDD372 pKa = 3.88ALVAAIDD379 pKa = 3.73EE380 pKa = 4.57DD381 pKa = 4.01

Molecular weight: 41.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I2GGM0|A0A2I2GGM0_9EURO Putative apoptosis-inducing factor OS=Aspergillus steynii IBT 23096 OX=1392250 GN=P170DRAFT_348257 PE=4 SV=1
SS1 pKa = 6.85HH2 pKa = 7.5KK3 pKa = 10.71SFRR6 pKa = 11.84TKK8 pKa = 10.45QKK10 pKa = 9.84LAKK13 pKa = 9.55AQKK16 pKa = 8.59QNRR19 pKa = 11.84PIPQWIRR26 pKa = 11.84LRR28 pKa = 11.84TGNTIRR34 pKa = 11.84YY35 pKa = 5.79NAKK38 pKa = 8.89RR39 pKa = 11.84RR40 pKa = 11.84HH41 pKa = 4.14WRR43 pKa = 11.84KK44 pKa = 7.41TRR46 pKa = 11.84LGII49 pKa = 4.46

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12963

2

12965

6073799

49

7729

468.5

51.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.406 ± 0.019

1.329 ± 0.008

5.683 ± 0.016

6.005 ± 0.022

3.882 ± 0.012

6.903 ± 0.016

2.478 ± 0.008

4.917 ± 0.015

4.407 ± 0.017

9.175 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.008

3.576 ± 0.01

6.099 ± 0.024

4.012 ± 0.014

6.171 ± 0.019

8.369 ± 0.022

5.796 ± 0.014

6.258 ± 0.016

1.52 ± 0.008

2.834 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski