Rhodocyclus tenuis (Rhodospirillum tenue)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Rhodocyclaceae; Rhodocyclus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L5JYB8|A0A6L5JYB8_RHOTE 50S ribosomal protein L25 OS=Rhodocyclus tenuis OX=1066 GN=rplY PE=3 SV=1
MM1 pKa = 7.17NAAVDD6 pKa = 3.88IPAPLVFTDD15 pKa = 3.8SAANKK20 pKa = 9.3VKK22 pKa = 10.43EE23 pKa = 4.42LILEE27 pKa = 4.32EE28 pKa = 4.51GNADD32 pKa = 3.32LKK34 pKa = 11.35LRR36 pKa = 11.84VFVSGGGCSGFQYY49 pKa = 10.9GFTFDD54 pKa = 4.48EE55 pKa = 4.64VAADD59 pKa = 4.88DD60 pKa = 3.94DD61 pKa = 4.15TAMEE65 pKa = 4.47KK66 pKa = 10.88NGVTLLIDD74 pKa = 3.7PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.64IDD87 pKa = 3.75YY88 pKa = 10.77TEE90 pKa = 4.43GLEE93 pKa = 4.25GSQFVIRR100 pKa = 11.84NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L5JYP0|A0A6L5JYP0_RHOTE Uncharacterized protein OS=Rhodocyclus tenuis OX=1066 GN=GHK24_11795 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.6QPSVIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.64RR14 pKa = 11.84THH16 pKa = 5.96GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2603

0

2603

876443

25

4251

336.7

36.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.488 ± 0.08

1.003 ± 0.017

5.449 ± 0.028

5.688 ± 0.043

3.588 ± 0.027

7.961 ± 0.042

2.188 ± 0.023

4.763 ± 0.037

2.962 ± 0.043

11.032 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.022

2.557 ± 0.029

5.105 ± 0.039

3.507 ± 0.028

7.458 ± 0.045

5.57 ± 0.041

4.973 ± 0.036

7.256 ± 0.037

1.26 ± 0.02

2.161 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski