Bat Paramyxovirus Epo_spe/AR1/DRC/2009
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I0E095|I0E095_9MONO Phosphoprotein OS=Bat Paramyxovirus Epo_spe/AR1/DRC/2009 OX=1112597 GN=P PE=3 SV=1
MM1 pKa = 7.49 SSVLKK6 pKa = 10.65 AFEE9 pKa = 4.31 RR10 pKa = 11.84 FTIEE14 pKa = 5.13 QEE16 pKa = 3.95 LQDD19 pKa = 4.13 RR20 pKa = 11.84 GEE22 pKa = 4.35 EE23 pKa = 4.24 GAIPPEE29 pKa = 4.18 TLKK32 pKa = 11.1 SAVKK36 pKa = 10.23 VFVINTPNPATRR48 pKa = 11.84 YY49 pKa = 10.09 QMLNFCLRR57 pKa = 11.84 IICSQNARR65 pKa = 11.84 ASHH68 pKa = 6.07 RR69 pKa = 11.84 VGALITLFSLPSAGMQNHH87 pKa = 6.05 IRR89 pKa = 11.84 LADD92 pKa = 4.27 RR93 pKa = 11.84 SPEE96 pKa = 3.9 AQIEE100 pKa = 4.18 RR101 pKa = 11.84 CEE103 pKa = 4.02 IDD105 pKa = 3.34 GFEE108 pKa = 4.47 PGTYY112 pKa = 10.03 RR113 pKa = 11.84 LIPNARR119 pKa = 11.84 ANLTANEE126 pKa = 3.72 IAAYY130 pKa = 10.25 ALLADD135 pKa = 5.13 DD136 pKa = 6.14 LPPTINNGTPYY147 pKa = 10.34 VHH149 pKa = 7.43 ADD151 pKa = 3.72 VEE153 pKa = 4.47 GQPCDD158 pKa = 3.97 EE159 pKa = 4.8 IEE161 pKa = 4.09 QFLDD165 pKa = 2.71 RR166 pKa = 11.84 CYY168 pKa = 11.21 SVLIQAWVMVCKK180 pKa = 10.67 CMTAYY185 pKa = 9.79 DD186 pKa = 4.06 QPAGSADD193 pKa = 3.45 RR194 pKa = 11.84 RR195 pKa = 11.84 FAKK198 pKa = 10.22 YY199 pKa = 8.47 QQQGRR204 pKa = 11.84 LEE206 pKa = 4.21 ARR208 pKa = 11.84 YY209 pKa = 8.28 MLQPEE214 pKa = 4.38 AQRR217 pKa = 11.84 LIQAAIRR224 pKa = 11.84 KK225 pKa = 8.85 SLVVRR230 pKa = 11.84 QYY232 pKa = 10.98 LTFEE236 pKa = 4.12 LQLARR241 pKa = 11.84 RR242 pKa = 11.84 QGLLSNRR249 pKa = 11.84 YY250 pKa = 8.0 YY251 pKa = 11.82 AMVGDD256 pKa = 3.41 IGKK259 pKa = 10.26 YY260 pKa = 9.45 IEE262 pKa = 4.47 NSGLTAFFLTLKK274 pKa = 10.27 YY275 pKa = 10.86 ALGTKK280 pKa = 9.03 WSPLSLAAFTGEE292 pKa = 4.05 LTKK295 pKa = 10.79 LRR297 pKa = 11.84 SLMMLYY303 pKa = 10.24 RR304 pKa = 11.84 DD305 pKa = 3.69 LGEE308 pKa = 3.91 QARR311 pKa = 11.84 YY312 pKa = 8.75 LALLEE317 pKa = 4.89 APQIMDD323 pKa = 4.55 FAPGGYY329 pKa = 9.29 PLIFSYY335 pKa = 11.38 AMGVGTVLDD344 pKa = 3.74 VQMRR348 pKa = 11.84 NYY350 pKa = 9.87 TYY352 pKa = 11.25 ARR354 pKa = 11.84 PFLNGYY360 pKa = 7.57 YY361 pKa = 9.8 FQIGVEE367 pKa = 4.14 TARR370 pKa = 11.84 RR371 pKa = 11.84 QQGTVDD377 pKa = 3.5 NRR379 pKa = 11.84 VADD382 pKa = 4.32 DD383 pKa = 4.52 LGLTPEE389 pKa = 3.96 QRR391 pKa = 11.84 TEE393 pKa = 3.77 VTQLVDD399 pKa = 3.29 RR400 pKa = 11.84 LARR403 pKa = 11.84 GRR405 pKa = 11.84 GAGIPGGPVNPFIPPAQQQQPAAVYY430 pKa = 10.97 DD431 pKa = 3.97 DD432 pKa = 3.99 MPALEE437 pKa = 4.68 EE438 pKa = 5.21 SEE440 pKa = 6.1 DD441 pKa = 5.18 DD442 pKa = 5.07 DD443 pKa = 7.39 DD444 pKa = 7.14 DD445 pKa = 6.69 DD446 pKa = 6.29 GGTRR450 pKa = 11.84 FQNGAQAPIARR461 pKa = 11.84 MGGQADD467 pKa = 4.15 LRR469 pKa = 11.84 AQHH472 pKa = 6.36 AQDD475 pKa = 5.33 PIQAQMFVPLYY486 pKa = 8.51 PQAGNMPTQQSTQINQIGGADD507 pKa = 3.54 QQDD510 pKa = 3.86 LLRR513 pKa = 11.84 YY514 pKa = 9.86 NDD516 pKa = 5.85 DD517 pKa = 3.36 NDD519 pKa = 3.6 QPQGARR525 pKa = 11.84 GEE527 pKa = 4.3 YY528 pKa = 10.84 GSIFSNNPTQVPQSQVGDD546 pKa = 3.42 WDD548 pKa = 3.74 EE549 pKa = 3.83
Molecular weight: 61.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.8
IPC2_protein 4.876
IPC_protein 4.825
Toseland 4.673
ProMoST 4.902
Dawson 4.787
Bjellqvist 4.952
Wikipedia 4.66
Rodwell 4.673
Grimsley 4.584
Solomon 4.787
Lehninger 4.736
Nozaki 4.889
DTASelect 5.08
Thurlkill 4.685
EMBOSS 4.685
Sillero 4.952
Patrickios 3.783
IPC_peptide 4.787
IPC2_peptide 4.94
IPC2.peptide.svr19 4.93
Protein with the highest isoelectric point:
>tr|I0E097|I0E097_9MONO Small hydrophobic protein OS=Bat Paramyxovirus Epo_spe/AR1/DRC/2009 OX=1112597 GN=SH PE=3 SV=1
MM1 pKa = 7.74 AGSQIKK7 pKa = 10.15 IPLPKK12 pKa = 10.27 PPDD15 pKa = 3.65 SDD17 pKa = 3.86 SQRR20 pKa = 11.84 LNAFPIVMAQEE31 pKa = 4.3 GKK33 pKa = 10.79 GRR35 pKa = 11.84 LLRR38 pKa = 11.84 QIRR41 pKa = 11.84 LRR43 pKa = 11.84 KK44 pKa = 9.32 LLSGDD49 pKa = 3.89 PSDD52 pKa = 4.12 HH53 pKa = 6.88 QITFVNTYY61 pKa = 9.68 GFIRR65 pKa = 11.84 AAPEE69 pKa = 3.51 TSEE72 pKa = 5.0 FISEE76 pKa = 4.21 SSPQKK81 pKa = 8.68 ITPVVTACMLSFGAGPVLDD100 pKa = 5.91 DD101 pKa = 3.97 PQQMLKK107 pKa = 10.74 ALDD110 pKa = 3.53 QADD113 pKa = 2.94 IRR115 pKa = 11.84 VRR117 pKa = 11.84 KK118 pKa = 6.83 TASDD122 pKa = 3.62 KK123 pKa = 10.74 EE124 pKa = 4.17 QVLFEE129 pKa = 4.55 INRR132 pKa = 11.84 IPNLFKK138 pKa = 10.5 HH139 pKa = 5.81 HH140 pKa = 6.43 QISSDD145 pKa = 3.34 HH146 pKa = 6.94 LIQASSDD153 pKa = 3.64 KK154 pKa = 10.67 YY155 pKa = 10.17 VKK157 pKa = 10.69 SPAKK161 pKa = 9.34 LTAGVNYY168 pKa = 9.75 IYY170 pKa = 10.64 CATFLSVTVCSASLKK185 pKa = 10.21 FRR187 pKa = 11.84 VARR190 pKa = 11.84 PLLAARR196 pKa = 11.84 SRR198 pKa = 11.84 LVRR201 pKa = 11.84 AVQMEE206 pKa = 3.97 VLLRR210 pKa = 11.84 VSCKK214 pKa = 9.82 KK215 pKa = 10.35 DD216 pKa = 3.26 SPMAKK221 pKa = 10.19 SMLTDD226 pKa = 4.09 PDD228 pKa = 4.12 GEE230 pKa = 4.48 GCIASVWFHH239 pKa = 6.66 LCNLCKK245 pKa = 10.4 GRR247 pKa = 11.84 NKK249 pKa = 10.08 LRR251 pKa = 11.84 SYY253 pKa = 10.8 DD254 pKa = 3.72 EE255 pKa = 4.62 NYY257 pKa = 10.12 FAAKK261 pKa = 8.66 CKK263 pKa = 10.35 KK264 pKa = 9.07 MNLTVSVGDD273 pKa = 3.24 MWGPTILVHH282 pKa = 7.2 ASGHH286 pKa = 5.89 IPTTAKK292 pKa = 10.4 PFFNSRR298 pKa = 11.84 GWVCHH303 pKa = 6.67 PIHH306 pKa = 6.64 QSSPSLAKK314 pKa = 9.57 TLWSSGCEE322 pKa = 3.65 IKK324 pKa = 10.75 GASAILQGSDD334 pKa = 3.31 YY335 pKa = 11.56 ASLAKK340 pKa = 9.8 TDD342 pKa = 4.63 DD343 pKa = 3.84 IIYY346 pKa = 10.72 SKK348 pKa = 10.88 IKK350 pKa = 9.61 IDD352 pKa = 4.63 KK353 pKa = 10.42 DD354 pKa = 3.05 AANYY358 pKa = 10.07 KK359 pKa = 9.33 GVSWNPFRR367 pKa = 11.84 KK368 pKa = 9.38 SASMNNLL375 pKa = 3.08
Molecular weight: 41.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.946
IPC2_protein 8.77
IPC_protein 8.639
Toseland 9.516
ProMoST 9.224
Dawson 9.75
Bjellqvist 9.502
Wikipedia 9.867
Rodwell 10.16
Grimsley 9.78
Solomon 9.78
Lehninger 9.75
Nozaki 9.736
DTASelect 9.414
Thurlkill 9.619
EMBOSS 9.94
Sillero 9.736
Patrickios 5.69
IPC_peptide 9.78
IPC2_peptide 8.229
IPC2.peptide.svr19 7.929
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4215
57
2261
702.5
78.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.216 ± 1.006
1.898 ± 0.289
5.219 ± 0.397
4.721 ± 0.561
3.369 ± 0.306
5.575 ± 0.813
1.708 ± 0.332
7.212 ± 0.51
4.555 ± 0.868
11.364 ± 1.274
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.752 ± 0.234
5.148 ± 0.54
5.362 ± 0.541
4.982 ± 0.963
4.958 ± 0.446
8.019 ± 0.807
6.358 ± 0.584
5.623 ± 0.368
1.186 ± 0.27
3.749 ± 0.481
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here