Torque teno zalophus virus 1 (ZcTTV)
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0JSK8|C0JSK8_ZCTTV Capsid protein OS=Torque teno zalophus virus 1 OX=1218490 PE=3 SV=1
MM1 pKa = 7.72 EE2 pKa = 6.87 DD3 pKa = 4.76 IRR5 pKa = 11.84 TLWKK9 pKa = 9.31 VTFDD13 pKa = 4.21 LLYY16 pKa = 10.73 DD17 pKa = 3.63 NNYY20 pKa = 9.52 FPSTGMYY27 pKa = 9.48 PCFDD31 pKa = 3.59 EE32 pKa = 6.91 KK33 pKa = 11.03 EE34 pKa = 4.09 ICLCLAWTLLLIGFSTLLILTIQLIISVRR63 pKa = 11.84 RR64 pKa = 11.84 LNGSVNALISIKK76 pKa = 10.6 NSAGVVTGQTISPKK90 pKa = 9.83 NAISEE95 pKa = 4.24 EE96 pKa = 4.25 VQAPEE101 pKa = 4.08 MEE103 pKa = 4.34 EE104 pKa = 3.96 TLQEE108 pKa = 4.23 VPLSEE113 pKa = 4.24 EE114 pKa = 4.21 SPLVGAQTQKK124 pKa = 10.07 MASPTKK130 pKa = 9.54 NACGKK135 pKa = 10.08 IPSFF139 pKa = 4.32
Molecular weight: 15.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.681
IPC2_protein 4.774
IPC_protein 4.596
Toseland 4.457
ProMoST 4.673
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.355
Rodwell 4.431
Grimsley 4.368
Solomon 4.507
Lehninger 4.457
Nozaki 4.622
DTASelect 4.698
Thurlkill 4.444
EMBOSS 4.368
Sillero 4.698
Patrickios 3.719
IPC_peptide 4.52
IPC2_peptide 4.685
IPC2.peptide.svr19 4.676
Protein with the highest isoelectric point:
>tr|C0JSK9|C0JSK9_ZCTTV Orf3 OS=Torque teno zalophus virus 1 OX=1218490 PE=4 SV=1
MM1 pKa = 7.66 PFRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 FRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 WRR13 pKa = 11.84 RR14 pKa = 11.84 PFRR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 8.63 HH20 pKa = 4.7 YY21 pKa = 10.11 RR22 pKa = 11.84 RR23 pKa = 11.84 NRR25 pKa = 11.84 HH26 pKa = 2.89 WWGRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 7.62 WRR36 pKa = 11.84 HH37 pKa = 4.46 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 MPAVRR45 pKa = 11.84 YY46 pKa = 9.21 HH47 pKa = 6.37 PSRR50 pKa = 11.84 RR51 pKa = 11.84 HH52 pKa = 4.91 KK53 pKa = 10.46 YY54 pKa = 8.39 IVIRR58 pKa = 11.84 GIEE61 pKa = 4.05 PLGNLCTDD69 pKa = 4.36 HH70 pKa = 6.35 TPSWLRR76 pKa = 11.84 ATPGRR81 pKa = 11.84 SFEE84 pKa = 4.56 SAGQTLGEE92 pKa = 4.27 WEE94 pKa = 4.56 GTWGAHH100 pKa = 4.21 HH101 pKa = 6.92 HH102 pKa = 6.23 SFAGLLLRR110 pKa = 11.84 AQCRR114 pKa = 11.84 FATFSGDD121 pKa = 2.86 WKK123 pKa = 10.58 SYY125 pKa = 11.12 DD126 pKa = 3.46 YY127 pKa = 11.03 IEE129 pKa = 4.42 YY130 pKa = 10.48 KK131 pKa = 10.77 GGTFYY136 pKa = 10.66 IPPQCTTFLFGIDD149 pKa = 3.54 PQFTKK154 pKa = 10.35 ISKK157 pKa = 9.86 EE158 pKa = 4.14 GEE160 pKa = 3.82 KK161 pKa = 10.2 EE162 pKa = 4.06 QPNEE166 pKa = 4.2 EE167 pKa = 4.25 TWLHH171 pKa = 6.03 PGWLLHH177 pKa = 6.16 QRR179 pKa = 11.84 GTHH182 pKa = 6.25 IIYY185 pKa = 10.87 SKK187 pKa = 10.44 DD188 pKa = 2.9 IKK190 pKa = 10.13 PWRR193 pKa = 11.84 RR194 pKa = 11.84 WYY196 pKa = 10.45 KK197 pKa = 10.65 LRR199 pKa = 11.84 VKK201 pKa = 10.31 PGPTWEE207 pKa = 4.83 GPYY210 pKa = 10.35 SLPNAFNFIMSQWWWSWLDD229 pKa = 3.49 FQHH232 pKa = 7.16 AFEE235 pKa = 6.04 DD236 pKa = 3.75 NTRR239 pKa = 11.84 SQICHH244 pKa = 7.2 ADD246 pKa = 3.47 PQNFSLFCGQKK257 pKa = 9.56 PWWFDD262 pKa = 3.07 STYY265 pKa = 10.72 KK266 pKa = 9.46 NTIEE270 pKa = 3.91 IPKK273 pKa = 9.93 RR274 pKa = 11.84 LVNKK278 pKa = 10.11 ACNAWVNRR286 pKa = 11.84 QEE288 pKa = 3.89 YY289 pKa = 8.69 MIKK292 pKa = 9.84 IGQKK296 pKa = 8.49 VTDD299 pKa = 4.03 KK300 pKa = 10.31 MLHH303 pKa = 6.27 PEE305 pKa = 4.66 DD306 pKa = 6.37 DD307 pKa = 3.94 ILCGNQSQQSQKK319 pKa = 11.09 KK320 pKa = 9.14 YY321 pKa = 10.18 FQQQNPGWGPFLPLLYY337 pKa = 11.01 NGDD340 pKa = 3.91 NCSLWFKK347 pKa = 10.25 YY348 pKa = 10.13 RR349 pKa = 11.84 YY350 pKa = 8.45 VFKK353 pKa = 11.23 VSGDD357 pKa = 3.36 CEE359 pKa = 4.18 YY360 pKa = 11.14 RR361 pKa = 11.84 KK362 pKa = 8.04 TPSTDD367 pKa = 2.87 LTQMVPTAPGPWNAEE382 pKa = 3.34 GDD384 pKa = 3.78 RR385 pKa = 11.84 PQIQSRR391 pKa = 11.84 SILKK395 pKa = 10.22 KK396 pKa = 9.43 SRR398 pKa = 11.84 KK399 pKa = 9.29 RR400 pKa = 11.84 PLDD403 pKa = 3.34 TADD406 pKa = 3.71 ILLGDD411 pKa = 4.11 TDD413 pKa = 5.57 DD414 pKa = 6.14 GILTEE419 pKa = 4.52 TGLEE423 pKa = 4.24 RR424 pKa = 11.84 ISGPSPKK431 pKa = 10.28 DD432 pKa = 3.3 LLCGMEE438 pKa = 4.04 NTPPKK443 pKa = 9.96 RR444 pKa = 11.84 VRR446 pKa = 11.84 FRR448 pKa = 11.84 QSDD451 pKa = 3.74 VLRR454 pKa = 11.84 KK455 pKa = 9.37 HH456 pKa = 5.66 KK457 pKa = 10.3 HH458 pKa = 5.95 RR459 pKa = 11.84 ILNLIDD465 pKa = 3.27 QLSRR469 pKa = 3.35
Molecular weight: 56.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.271
IPC2_protein 9.428
IPC_protein 9.648
Toseland 10.16
ProMoST 9.897
Dawson 10.35
Bjellqvist 10.043
Wikipedia 10.526
Rodwell 10.687
Grimsley 10.423
Solomon 10.379
Lehninger 10.35
Nozaki 10.189
DTASelect 10.028
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.277
Patrickios 10.233
IPC_peptide 10.379
IPC2_peptide 8.946
IPC2.peptide.svr19 8.411
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
770
139
469
256.7
29.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.636 ± 0.582
1.948 ± 0.435
4.545 ± 0.532
5.974 ± 1.467
4.026 ± 0.814
5.844 ± 0.867
3.117 ± 0.854
5.325 ± 0.985
5.714 ± 0.593
8.182 ± 1.107
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.948 ± 0.386
3.896 ± 0.345
6.494 ± 0.658
5.974 ± 1.049
7.532 ± 2.624
8.182 ± 2.359
7.143 ± 1.483
3.506 ± 0.978
3.506 ± 1.176
3.506 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here