bacterium K02(2017)

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202E270|A0A202E270_9BACT S4 RNA-binding domain-containing protein OS=bacterium K02(2017) OX=1932696 GN=BVY03_01135 PE=4 SV=1
MM1 pKa = 7.11VPGVKK6 pKa = 9.88KK7 pKa = 10.64ISFLFITLLVLLVGSLVVLAADD29 pKa = 5.02IIDD32 pKa = 4.0DD33 pKa = 4.12TEE35 pKa = 5.19AEE37 pKa = 4.33FDD39 pKa = 3.35NGSYY43 pKa = 10.86TNVDD47 pKa = 3.98FDD49 pKa = 4.42TGNTWVEE56 pKa = 3.95LTAPATNAYY65 pKa = 10.42GNFEE69 pKa = 4.14SDD71 pKa = 3.54IYY73 pKa = 11.43DD74 pKa = 3.61SGGTATWNTISWVPNRR90 pKa = 11.84PSGKK94 pKa = 9.48QLLNDD99 pKa = 4.13GGTEE103 pKa = 4.04GSSYY107 pKa = 10.92DD108 pKa = 3.42INNISMSGNVSVWHH122 pKa = 6.31FNASSWNGTAGEE134 pKa = 4.27VVDD137 pKa = 6.27DD138 pKa = 4.55NDD140 pKa = 3.79ASEE143 pKa = 4.13NAHH146 pKa = 6.86DD147 pKa = 4.45GVSVTSVTTDD157 pKa = 2.87STGMFNTRR165 pKa = 11.84SAEE168 pKa = 3.76FDD170 pKa = 3.54DD171 pKa = 5.19DD172 pKa = 4.2GGRR175 pKa = 11.84LEE177 pKa = 4.97IADD180 pKa = 4.66HH181 pKa = 6.77NDD183 pKa = 2.71FDD185 pKa = 4.91ASSFTLEE192 pKa = 3.28AWVYY196 pKa = 10.16PKK198 pKa = 9.53STPDD202 pKa = 3.14WNVVAIKK209 pKa = 9.22ATNDD213 pKa = 3.2AFDD216 pKa = 5.44DD217 pKa = 4.0GWGMVIQGSEE227 pKa = 3.86VKK229 pKa = 10.47FYY231 pKa = 11.43VQDD234 pKa = 3.49QANYY238 pKa = 8.18SASIAVTTNEE248 pKa = 3.73WNHH251 pKa = 5.81IVGTYY256 pKa = 10.18DD257 pKa = 3.13GSNVRR262 pKa = 11.84SYY264 pKa = 11.61INGTIDD270 pKa = 3.13STVAYY275 pKa = 7.24TGPYY279 pKa = 10.03DD280 pKa = 3.81GTSTALMIGGDD291 pKa = 3.69NFYY294 pKa = 11.22DD295 pKa = 3.65SDD297 pKa = 3.62TWDD300 pKa = 3.66GYY302 pKa = 9.64IDD304 pKa = 3.68EE305 pKa = 4.46VAFYY309 pKa = 11.04DD310 pKa = 3.88VVLDD314 pKa = 4.08AGEE317 pKa = 3.69INARR321 pKa = 11.84YY322 pKa = 9.39KK323 pKa = 10.71RR324 pKa = 11.84GIQRR328 pKa = 11.84VRR330 pKa = 11.84FQVRR334 pKa = 11.84SCDD337 pKa = 3.56DD338 pKa = 3.7AACSGEE344 pKa = 4.24NFEE347 pKa = 5.67GPDD350 pKa = 3.65GTTGTYY356 pKa = 10.64YY357 pKa = 10.64SASSNLITATPTATLTNVDD376 pKa = 3.81DD377 pKa = 4.24NQYY380 pKa = 9.47FQYY383 pKa = 10.85KK384 pKa = 9.93IFFEE388 pKa = 4.34TDD390 pKa = 3.0DD391 pKa = 3.69TGLTPEE397 pKa = 4.41INSVTVNYY405 pKa = 10.61DD406 pKa = 3.33LVSSDD411 pKa = 4.06FVSSKK416 pKa = 10.59QKK418 pKa = 10.47ISDD421 pKa = 3.51TAGVFTAVLDD431 pKa = 3.67NSDD434 pKa = 3.79YY435 pKa = 11.39FGVTISNIGDD445 pKa = 3.97LDD447 pKa = 3.89GDD449 pKa = 4.65SINDD453 pKa = 4.66LIVGAHH459 pKa = 5.87QDD461 pKa = 3.64DD462 pKa = 4.82DD463 pKa = 4.35GGADD467 pKa = 3.28RR468 pKa = 11.84GAVYY472 pKa = 10.89VLFLEE477 pKa = 4.43SDD479 pKa = 3.85GTVSSFQKK487 pKa = 10.59ISDD490 pKa = 3.7TAGSFTAVLDD500 pKa = 3.55NTDD503 pKa = 3.29YY504 pKa = 11.33FGISAASIGDD514 pKa = 3.49IDD516 pKa = 3.99TDD518 pKa = 4.58GINDD522 pKa = 3.49IAVGAFFDD530 pKa = 5.99DD531 pKa = 5.16DD532 pKa = 4.01GGTNKK537 pKa = 10.22GAVYY541 pKa = 10.42ILFLEE546 pKa = 4.27NDD548 pKa = 3.92GTVSSFQKK556 pKa = 10.59ISDD559 pKa = 3.7TAGSFTASFTVTDD572 pKa = 4.04LFGRR576 pKa = 11.84SVASMGDD583 pKa = 3.5VNGDD587 pKa = 3.95SVPDD591 pKa = 3.67IAVGAYY597 pKa = 9.94YY598 pKa = 10.58DD599 pKa = 4.84DD600 pKa = 6.12DD601 pKa = 5.74GGTDD605 pKa = 2.98RR606 pKa = 11.84GAVYY610 pKa = 10.25IIFLNNDD617 pKa = 3.19GTVKK621 pKa = 10.43SFQKK625 pKa = 10.62ISDD628 pKa = 3.94TEE630 pKa = 4.41GSFTGVLDD638 pKa = 3.42NTDD641 pKa = 3.38YY642 pKa = 11.35FGVSVASIKK651 pKa = 10.56DD652 pKa = 3.68LNGDD656 pKa = 4.67GINEE660 pKa = 4.28LAVGSYY666 pKa = 10.94YY667 pKa = 10.85DD668 pKa = 4.56DD669 pKa = 5.45DD670 pKa = 5.3GGSEE674 pKa = 3.9RR675 pKa = 11.84GAVYY679 pKa = 11.23VMFLNNNGTVSSFQKK694 pKa = 10.6ISDD697 pKa = 3.91TEE699 pKa = 4.36GSFTAVLDD707 pKa = 3.68NTNGFGVSVDD717 pKa = 3.64GLNDD721 pKa = 3.48FDD723 pKa = 6.23GDD725 pKa = 4.42GITDD729 pKa = 3.95LMVGAHH735 pKa = 6.54SDD737 pKa = 3.59NDD739 pKa = 3.71GGTDD743 pKa = 3.04NGAIYY748 pKa = 10.68VLYY751 pKa = 10.6LNANGTVSSYY761 pKa = 11.37EE762 pKa = 4.19KK763 pKa = 10.57ISDD766 pKa = 3.78TEE768 pKa = 4.3GGFTGVLDD776 pKa = 3.59NSDD779 pKa = 3.67YY780 pKa = 11.35FGISAANIADD790 pKa = 4.1LDD792 pKa = 4.22GDD794 pKa = 4.38GVTDD798 pKa = 3.81IAIGAYY804 pKa = 9.97GDD806 pKa = 3.8ADD808 pKa = 3.52GGLNRR813 pKa = 11.84GAVHH817 pKa = 7.23ILNISQPDD825 pKa = 3.94SYY827 pKa = 11.48SVGSITINAQMAINRR842 pKa = 11.84NTNNANTKK850 pKa = 7.73VTITFTIAGAIPVNGDD866 pKa = 2.4IDD868 pKa = 4.42IIFPTGFILTEE879 pKa = 4.02VGSGDD884 pKa = 3.53VSEE887 pKa = 5.05ADD889 pKa = 3.34SDD891 pKa = 4.12GGTLTTSVSGQRR903 pKa = 11.84MTINCGSSITASTEE917 pKa = 3.6LALTIDD923 pKa = 3.76NVVNSASAGDD933 pKa = 3.94YY934 pKa = 10.09EE935 pKa = 4.41GLIIEE940 pKa = 4.63TQDD943 pKa = 3.21ASDD946 pKa = 3.75VVIDD950 pKa = 4.15LVEE953 pKa = 5.01SDD955 pKa = 4.07GVTVIQKK962 pKa = 8.98PRR964 pKa = 11.84RR965 pKa = 11.84ILTSQQ970 pKa = 2.86

Molecular weight: 102.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DZ42|A0A202DZ42_9BACT Replicative DNA helicase OS=bacterium K02(2017) OX=1932696 GN=BVY03_03785 PE=3 SV=1
MM1 pKa = 7.79KK2 pKa = 10.07FRR4 pKa = 11.84KK5 pKa = 9.62KK6 pKa = 10.52LLSKK10 pKa = 10.69QKK12 pKa = 10.24LRR14 pKa = 11.84PKK16 pKa = 10.48LRR18 pKa = 11.84VKK20 pKa = 10.14QRR22 pKa = 11.84QKK24 pKa = 10.55QRR26 pKa = 11.84PKK28 pKa = 9.84LRR30 pKa = 11.84VRR32 pKa = 11.84QRR34 pKa = 11.84VRR36 pKa = 11.84QRR38 pKa = 11.84QKK40 pKa = 8.62PKK42 pKa = 10.03LRR44 pKa = 11.84VRR46 pKa = 11.84QRR48 pKa = 11.84QKK50 pKa = 10.61QKK52 pKa = 10.62QKK54 pKa = 10.97LRR56 pKa = 11.84LKK58 pKa = 10.69LKK60 pKa = 10.25LRR62 pKa = 11.84HH63 pKa = 5.51LQNLFVEE70 pKa = 4.89MEE72 pKa = 3.99NN73 pKa = 3.82

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1213

0

1213

401908

24

6113

331.3

37.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.494 ± 0.082

0.997 ± 0.035

5.875 ± 0.084

5.778 ± 0.068

4.712 ± 0.07

6.293 ± 0.085

2.037 ± 0.042

8.148 ± 0.133

7.719 ± 0.145

9.888 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.103 ± 0.04

6.022 ± 0.079

3.57 ± 0.061

3.988 ± 0.058

3.443 ± 0.065

7.026 ± 0.075

5.548 ± 0.122

5.886 ± 0.058

0.927 ± 0.025

3.544 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski