Pepino mosaic virus
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8JSN4|Q8JSN4_9VIRU ORF1 protein OS=Pepino mosaic virus OX=112229 PE=4 SV=1
MM1 pKa = 7.35 EE2 pKa = 5.96 RR3 pKa = 11.84 STLINLLQLHH13 pKa = 6.18 HH14 pKa = 6.95 FEE16 pKa = 5.1 PKK18 pKa = 10.42 LSVEE22 pKa = 4.17 GTIVVHH28 pKa = 6.84 GIAGTGKK35 pKa = 7.05 TTLLRR40 pKa = 11.84 TLFSAYY46 pKa = 8.77 PSLVIGSPRR55 pKa = 11.84 PCYY58 pKa = 10.13 LDD60 pKa = 3.3 KK61 pKa = 11.07 QNKK64 pKa = 8.56 ISQVCLSCFPNTHH77 pKa = 6.59 CDD79 pKa = 3.19 IVDD82 pKa = 3.84 EE83 pKa = 4.24 YY84 pKa = 11.51 HH85 pKa = 6.9 LLEE88 pKa = 5.05 SFLEE92 pKa = 4.15 PKK94 pKa = 10.3 LAIFGDD100 pKa = 4.36 PCQCTYY106 pKa = 10.57 IEE108 pKa = 4.43 RR109 pKa = 11.84 LRR111 pKa = 11.84 VPHH114 pKa = 5.05 YY115 pKa = 9.89 TSFRR119 pKa = 11.84 THH121 pKa = 7.07 RR122 pKa = 11.84 FGKK125 pKa = 8.37 STAEE129 pKa = 3.82 ILNKK133 pKa = 10.34 LFDD136 pKa = 4.75 LNIVSVKK143 pKa = 10.62 KK144 pKa = 10.56 EE145 pKa = 3.7 DD146 pKa = 5.14 DD147 pKa = 3.11 IVEE150 pKa = 4.38 FFNPFEE156 pKa = 4.28 VDD158 pKa = 3.24 PTEE161 pKa = 5.01 HH162 pKa = 6.6 ISASEE167 pKa = 4.16 EE168 pKa = 3.87 EE169 pKa = 4.43 VLDD172 pKa = 4.17 FVSDD176 pKa = 3.83 QVVTTSSEE184 pKa = 3.87 EE185 pKa = 3.84 LAGLEE190 pKa = 4.07 FAEE193 pKa = 4.59 TTFYY197 pKa = 10.41 CTTLAAAVAEE207 pKa = 4.45 NPAKK211 pKa = 10.14 TFISLTRR218 pKa = 11.84 HH219 pKa = 3.93 THH221 pKa = 5.71 KK222 pKa = 10.47 LTIGEE227 pKa = 4.14 LNARR231 pKa = 11.84 SNSS234 pKa = 3.46
Molecular weight: 26.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.162
IPC2_protein 5.207
IPC_protein 5.169
Toseland 5.308
ProMoST 5.385
Dawson 5.258
Bjellqvist 5.347
Wikipedia 5.156
Rodwell 5.207
Grimsley 5.308
Solomon 5.258
Lehninger 5.219
Nozaki 5.423
DTASelect 5.55
Thurlkill 5.385
EMBOSS 5.308
Sillero 5.512
Patrickios 3.719
IPC_peptide 5.27
IPC2_peptide 5.525
IPC2.peptide.svr19 5.424
Protein with the highest isoelectric point:
>tr|Q8JSN2|Q8JSN2_9VIRU Movement protein TGBp3 OS=Pepino mosaic virus OX=112229 GN=TGBp3 PE=3 SV=1
MM1 pKa = 7.76 PGLTPRR7 pKa = 11.84 ADD9 pKa = 3.32 LTDD12 pKa = 3.49 TYY14 pKa = 10.87 KK15 pKa = 10.66 IIAIAFLLSACIYY28 pKa = 8.34 FQNSHH33 pKa = 5.34 YY34 pKa = 10.83 QPVAGDD40 pKa = 3.55 NLHH43 pKa = 6.77 RR44 pKa = 11.84 LPFGGQYY51 pKa = 10.32 QDD53 pKa = 3.23 GTKK56 pKa = 10.04 KK57 pKa = 10.19 ISYY60 pKa = 9.45 FPQQQSYY67 pKa = 7.44 FHH69 pKa = 6.99 SGNKK73 pKa = 10.02 LNVLILIFILTLGIVLTNKK92 pKa = 9.85 FSFSFSRR99 pKa = 11.84 TTHH102 pKa = 4.58 QHH104 pKa = 3.95 SCYY107 pKa = 8.65 NTHH110 pKa = 6.59 SATNNTQPLSGHH122 pKa = 6.13 HH123 pKa = 6.13
Molecular weight: 13.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.751
IPC2_protein 8.726
IPC_protein 8.77
Toseland 8.946
ProMoST 8.931
Dawson 9.297
Bjellqvist 9.238
Wikipedia 9.516
Rodwell 9.37
Grimsley 9.355
Solomon 9.458
Lehninger 9.414
Nozaki 9.209
DTASelect 9.136
Thurlkill 9.165
EMBOSS 9.399
Sillero 9.355
Patrickios 2.371
IPC_peptide 9.443
IPC2_peptide 8.214
IPC2.peptide.svr19 7.852
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2117
84
1439
423.4
47.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.314 ± 1.144
1.606 ± 0.251
4.818 ± 0.454
5.668 ± 0.879
5.102 ± 0.35
4.251 ± 0.334
3.637 ± 0.443
6.141 ± 0.492
6.046 ± 0.771
8.88 ± 0.589
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.031 ± 0.408
5.574 ± 0.367
5.243 ± 0.489
4.44 ± 0.571
3.826 ± 0.42
7.227 ± 0.79
7.227 ± 0.611
5.338 ± 0.346
0.897 ± 0.209
3.732 ± 0.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here