Ustilaginoidea virens RNA virus 1
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9QS71|M9QS71_9VIRU Putative coat protein OS=Ustilaginoidea virens RNA virus 1 OX=1312445 PE=4 SV=1
MM1 pKa = 7.34 SALSTFLSGGVVAKK15 pKa = 10.88 GPLKK19 pKa = 9.87 NNEE22 pKa = 3.24 VRR24 pKa = 11.84 RR25 pKa = 11.84 YY26 pKa = 6.98 YY27 pKa = 8.24 TTLISEE33 pKa = 4.17 ATLNGVQDD41 pKa = 3.83 VAGKK45 pKa = 7.52 TVVWKK50 pKa = 10.67 VGYY53 pKa = 9.9 AYY55 pKa = 10.73 SGTAGIDD62 pKa = 3.18 YY63 pKa = 10.79 DD64 pKa = 4.19 GVYY67 pKa = 10.04 KK68 pKa = 10.56 IHH70 pKa = 6.69 AEE72 pKa = 4.08 KK73 pKa = 10.9 VRR75 pKa = 11.84 ADD77 pKa = 3.46 PKK79 pKa = 10.8 AYY81 pKa = 9.64 PNSKK85 pKa = 9.67 KK86 pKa = 10.37 RR87 pKa = 11.84 NEE89 pKa = 4.06 LLKK92 pKa = 10.43 PLIRR96 pKa = 11.84 EE97 pKa = 4.43 AYY99 pKa = 8.33 NLKK102 pKa = 10.66 DD103 pKa = 3.84 SEE105 pKa = 4.39 EE106 pKa = 4.31 LPIPAALVCPKK117 pKa = 10.27 SVAIRR122 pKa = 11.84 LQTPYY127 pKa = 9.93 PVNEE131 pKa = 4.72 AISAEE136 pKa = 4.07 FANAARR142 pKa = 11.84 RR143 pKa = 11.84 YY144 pKa = 8.36 SGVVGTHH151 pKa = 5.46 GTADD155 pKa = 3.21 FCGIVAQLAKK165 pKa = 10.52 GLAFFASTGGLTMRR179 pKa = 11.84 DD180 pKa = 3.27 LAGGNSYY187 pKa = 11.29 SYY189 pKa = 9.98 MAVGNHH195 pKa = 5.33 TAPLVASTTSIWVPRR210 pKa = 11.84 YY211 pKa = 9.92 AEE213 pKa = 4.33 SLMAPNIMAALVAAGSAVGSTIVTDD238 pKa = 4.5 LLPTDD243 pKa = 3.75 VTNTPLLTEE252 pKa = 4.47 ANHH255 pKa = 6.62 LEE257 pKa = 4.21 LAAGVYY263 pKa = 9.56 HH264 pKa = 7.51 ALRR267 pKa = 11.84 VLGTNMEE274 pKa = 4.42 ANGAGQLFAYY284 pKa = 9.7 AVTVGIHH291 pKa = 5.33 SVVTVVGMTDD301 pKa = 2.73 EE302 pKa = 4.44 GAYY305 pKa = 9.32 MRR307 pKa = 11.84 DD308 pKa = 3.47 VFRR311 pKa = 11.84 STSFAPSYY319 pKa = 11.02 GGISTVLPEE328 pKa = 3.73 WHH330 pKa = 6.7 GFPRR334 pKa = 11.84 PADD337 pKa = 3.38 SSLAGWVGLVDD348 pKa = 5.48 SIALGTAASVAIADD362 pKa = 3.92 PCVAIADD369 pKa = 4.31 RR370 pKa = 11.84 YY371 pKa = 10.67 YY372 pKa = 9.48 PTTLTGRR379 pKa = 11.84 YY380 pKa = 8.77 EE381 pKa = 4.16 YY382 pKa = 10.94 GGEE385 pKa = 3.88 PGMGYY390 pKa = 10.38 VGDD393 pKa = 4.26 EE394 pKa = 3.66 NDD396 pKa = 3.41 ARR398 pKa = 11.84 IISGKK403 pKa = 9.96 IMQLAPSFTRR413 pKa = 11.84 NYY415 pKa = 9.92 FKK417 pKa = 11.13 VLARR421 pKa = 11.84 LFSVTPHH428 pKa = 6.34 EE429 pKa = 4.39 NSSVGLTHH437 pKa = 7.13 MIHH440 pKa = 6.61 AFSSEE445 pKa = 3.89 TLRR448 pKa = 11.84 DD449 pKa = 3.69 SRR451 pKa = 11.84 HH452 pKa = 5.41 LQHH455 pKa = 6.99 PSVSPFYY462 pKa = 9.42 WVEE465 pKa = 3.5 PTGIITFDD473 pKa = 3.36 TSDD476 pKa = 3.62 FTATAAGFGTLATPSQPGTIPMFEE500 pKa = 4.07 RR501 pKa = 11.84 AEE503 pKa = 4.14 VTQNVGDD510 pKa = 3.88 VSDD513 pKa = 4.64 VLVAWRR519 pKa = 11.84 SARR522 pKa = 11.84 TSGLAIHH529 pKa = 6.92 LNNHH533 pKa = 5.15 VEE535 pKa = 4.09 AGMEE539 pKa = 4.29 NIRR542 pKa = 11.84 ITGCDD547 pKa = 3.59 PNSWAQLGGASQSVSNRR564 pKa = 11.84 VTAGNDD570 pKa = 2.93 LASYY574 pKa = 8.2 MWARR578 pKa = 11.84 SDD580 pKa = 3.56 VGVPAPGEE588 pKa = 3.73 AMYY591 pKa = 10.69 LGEE594 pKa = 5.39 AISLLVRR601 pKa = 11.84 HH602 pKa = 6.32 DD603 pKa = 3.96 TLDD606 pKa = 3.54 GNTWANTPNHH616 pKa = 5.92 VPRR619 pKa = 11.84 DD620 pKa = 3.71 VEE622 pKa = 4.07 FGNNVTIRR630 pKa = 11.84 VGLLAPYY637 pKa = 9.96 GVGPIGPNRR646 pKa = 11.84 KK647 pKa = 9.44 ANRR650 pKa = 11.84 AFTAALRR657 pKa = 11.84 ALDD660 pKa = 3.75 AARR663 pKa = 11.84 EE664 pKa = 4.22 SGMIRR669 pKa = 11.84 APKK672 pKa = 10.1 LCGTIKK678 pKa = 10.29 LTDD681 pKa = 3.37 QAFGRR686 pKa = 11.84 QAAVPVAIAPPSPKK700 pKa = 10.04 AANRR704 pKa = 11.84 PVTTVSQPPQSDD716 pKa = 3.25 ARR718 pKa = 11.84 PVEE721 pKa = 3.98 PQNYY725 pKa = 9.03 AEE727 pKa = 4.9 SPTAHH732 pKa = 7.06 VYY734 pKa = 8.2 TQGPKK739 pKa = 8.66 TAPGPAQSVIASVRR753 pKa = 11.84 QMAAEE758 pKa = 4.31 EE759 pKa = 4.2 SAEE762 pKa = 4.27 TASVAAAPQAGAAQQ776 pKa = 3.55
Molecular weight: 81.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.958
IPC2_protein 5.919
IPC_protein 6.008
Toseland 6.465
ProMoST 6.364
Dawson 6.313
Bjellqvist 6.3
Wikipedia 6.326
Rodwell 6.3
Grimsley 6.649
Solomon 6.313
Lehninger 6.3
Nozaki 6.576
DTASelect 6.781
Thurlkill 6.795
EMBOSS 6.766
Sillero 6.693
Patrickios 4.012
IPC_peptide 6.326
IPC2_peptide 6.649
IPC2.peptide.svr19 6.606
Protein with the highest isoelectric point:
>tr|M9QS71|M9QS71_9VIRU Putative coat protein OS=Ustilaginoidea virens RNA virus 1 OX=1312445 PE=4 SV=1
MM1 pKa = 7.37 EE2 pKa = 4.67 SAAVRR7 pKa = 11.84 ANEE10 pKa = 3.84 LGAYY14 pKa = 8.9 GHH16 pKa = 7.0 ALLRR20 pKa = 11.84 FLPPSEE26 pKa = 4.32 VCDD29 pKa = 5.17 DD30 pKa = 3.28 IAMSNTDD37 pKa = 4.1 AQWHH41 pKa = 5.17 MLGSLGTKK49 pKa = 9.3 YY50 pKa = 10.98 GYY52 pKa = 9.91 ISQVACSLLVCNTPFQVMLHH72 pKa = 5.8 EE73 pKa = 4.09 RR74 pKa = 11.84 HH75 pKa = 5.84 IVEE78 pKa = 4.76 LLHH81 pKa = 6.62 LVKK84 pKa = 10.42 PVLTPHH90 pKa = 6.82 CEE92 pKa = 3.84 YY93 pKa = 11.03 DD94 pKa = 3.64 VTSPCSACIEE104 pKa = 4.18 LDD106 pKa = 3.27 SLGRR110 pKa = 11.84 VGPGSTVLAFKK121 pKa = 11.06 GDD123 pKa = 3.61 IAHH126 pKa = 6.47 TKK128 pKa = 10.24 CNIAFSDD135 pKa = 3.62 AWVDD139 pKa = 3.53 LCNNSPAIASQVQRR153 pKa = 11.84 SGRR156 pKa = 11.84 ALHH159 pKa = 6.63 GCYY162 pKa = 9.66 NDD164 pKa = 3.31 QGTAIIMYY172 pKa = 7.82 TAALFQHH179 pKa = 6.83 LKK181 pKa = 11.05 GEE183 pKa = 4.28 AFPYY187 pKa = 9.95 SYY189 pKa = 10.88 HH190 pKa = 6.51 FVRR193 pKa = 11.84 NPHH196 pKa = 6.1 DD197 pKa = 3.88 AKK199 pKa = 11.11 AFSTILKK206 pKa = 10.69 AMGANATPLGSVLTEE221 pKa = 4.01 ANTLTGRR228 pKa = 11.84 GVNPIDD234 pKa = 3.55 VEE236 pKa = 3.94 AAGRR240 pKa = 11.84 DD241 pKa = 3.09 RR242 pKa = 11.84 TQYY245 pKa = 11.52 DD246 pKa = 3.66 KK247 pKa = 10.88 IVRR250 pKa = 11.84 DD251 pKa = 3.93 LFHH254 pKa = 8.02 ADD256 pKa = 2.97 EE257 pKa = 4.98 GKK259 pKa = 10.39 LRR261 pKa = 11.84 EE262 pKa = 4.6 AIRR265 pKa = 11.84 TILRR269 pKa = 11.84 EE270 pKa = 4.24 EE271 pKa = 3.95 IGSANVEE278 pKa = 4.29 FPSLEE283 pKa = 4.16 EE284 pKa = 3.99 YY285 pKa = 10.59 FSQRR289 pKa = 11.84 WLWTVNGSHH298 pKa = 6.85 NFTTQRR304 pKa = 11.84 FYY306 pKa = 11.36 DD307 pKa = 4.33 PEE309 pKa = 3.99 DD310 pKa = 3.5 HH311 pKa = 6.94 RR312 pKa = 11.84 PPGAARR318 pKa = 11.84 MFRR321 pKa = 11.84 RR322 pKa = 11.84 AYY324 pKa = 10.22 AEE326 pKa = 3.96 CTSYY330 pKa = 11.62 DD331 pKa = 4.84 RR332 pKa = 11.84 VAAWNGKK339 pKa = 8.08 SFFSPSAKK347 pKa = 10.05 LEE349 pKa = 4.3 HH350 pKa = 6.37 GKK352 pKa = 10.03 SRR354 pKa = 11.84 AIFAGDD360 pKa = 3.12 TLTYY364 pKa = 10.54 FRR366 pKa = 11.84 FQHH369 pKa = 5.61 FLKK372 pKa = 10.42 HH373 pKa = 7.16 VEE375 pKa = 4.18 DD376 pKa = 3.96 VWTGKK381 pKa = 9.76 HH382 pKa = 4.99 VLLNPGRR389 pKa = 11.84 GGMYY393 pKa = 10.92 GMLKK397 pKa = 10.17 RR398 pKa = 11.84 IRR400 pKa = 11.84 GISAGTGVHH409 pKa = 5.67 TMMDD413 pKa = 3.51 YY414 pKa = 11.28 TDD416 pKa = 4.64 FNSAHH421 pKa = 6.31 TNASMRR427 pKa = 11.84 ILFEE431 pKa = 3.88 EE432 pKa = 4.2 ATSYY436 pKa = 11.01 VGYY439 pKa = 10.56 DD440 pKa = 3.46 PDD442 pKa = 4.36 MGALLANSFDD452 pKa = 3.48 NSYY455 pKa = 10.07 MVTSDD460 pKa = 3.8 GPRR463 pKa = 11.84 KK464 pKa = 9.44 IAGTLMSGHH473 pKa = 7.16 RR474 pKa = 11.84 ATTFINSVLNRR485 pKa = 11.84 AYY487 pKa = 10.87 LLVVNPSILRR497 pKa = 11.84 LPAVHH502 pKa = 6.63 VGDD505 pKa = 4.97 DD506 pKa = 4.08 VYY508 pKa = 11.48 LSPPSLHH515 pKa = 6.8 AAAEE519 pKa = 4.1 LMDD522 pKa = 4.52 DD523 pKa = 3.48 VRR525 pKa = 11.84 MSGLRR530 pKa = 11.84 MNPLKK535 pKa = 10.72 QSVGLITGEE544 pKa = 4.1 FLRR547 pKa = 11.84 MAFGSGSAFGYY558 pKa = 10.32 APRR561 pKa = 11.84 AIASMISGNWTNSNEE576 pKa = 4.04 LTRR579 pKa = 11.84 RR580 pKa = 11.84 EE581 pKa = 3.98 QVEE584 pKa = 4.2 NLVTTSWSLTNRR596 pKa = 11.84 CRR598 pKa = 11.84 NPAAAVLATTALSKK612 pKa = 10.86 RR613 pKa = 11.84 CGLSKK618 pKa = 10.79 CDD620 pKa = 5.53 AEE622 pKa = 4.44 ALLNGTLALGSGPARR637 pKa = 11.84 AGKK640 pKa = 9.22 HH641 pKa = 3.52 VYY643 pKa = 10.02 KK644 pKa = 10.3 RR645 pKa = 11.84 VEE647 pKa = 3.96 IPAPPVSLIPKK658 pKa = 8.91 EE659 pKa = 4.19 VLSKK663 pKa = 10.83 LPSRR667 pKa = 11.84 ATDD670 pKa = 3.46 DD671 pKa = 3.39 YY672 pKa = 11.41 LRR674 pKa = 11.84 NHH676 pKa = 6.83 TSTLEE681 pKa = 3.92 RR682 pKa = 11.84 TALQLLGTSPKK693 pKa = 9.33 VTMLEE698 pKa = 3.7 SSYY701 pKa = 11.56 AKK703 pKa = 9.38 TLRR706 pKa = 11.84 PVDD709 pKa = 4.24 EE710 pKa = 4.83 VPLPMGYY717 pKa = 10.49 AKK719 pKa = 10.57 EE720 pKa = 4.13 WMFGPASVQRR730 pKa = 11.84 GVSIDD735 pKa = 3.24 EE736 pKa = 4.03 CRR738 pKa = 11.84 NRR740 pKa = 11.84 DD741 pKa = 3.87 VIHH744 pKa = 6.67 GALSEE749 pKa = 4.29 YY750 pKa = 10.29 PLIVLFKK757 pKa = 10.18 EE758 pKa = 4.29 QFTKK762 pKa = 10.7 EE763 pKa = 3.62 QLAILLRR770 pKa = 11.84 MKK772 pKa = 10.66 CDD774 pKa = 3.64 LYY776 pKa = 11.03 TNDD779 pKa = 2.89 VRR781 pKa = 11.84 VAAFGAEE788 pKa = 3.68 ARR790 pKa = 11.84 GMVVEE795 pKa = 4.91 GWLPRR800 pKa = 11.84 ADD802 pKa = 3.47 VQHH805 pKa = 6.72 ASRR808 pKa = 11.84 RR809 pKa = 11.84 ATEE812 pKa = 4.24 MVLSTSINLYY822 pKa = 10.0 FF823 pKa = 4.81
Molecular weight: 90.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.927
IPC2_protein 7.176
IPC_protein 7.073
Toseland 6.956
ProMoST 7.614
Dawson 7.717
Bjellqvist 8.083
Wikipedia 7.644
Rodwell 7.732
Grimsley 7.073
Solomon 7.746
Lehninger 7.775
Nozaki 8.287
DTASelect 7.922
Thurlkill 7.951
EMBOSS 7.98
Sillero 8.229
Patrickios 4.202
IPC_peptide 7.746
IPC2_peptide 7.439
IPC2.peptide.svr19 7.184
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1599
776
823
799.5
86.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.882 ± 1.336
1.251 ± 0.425
4.378 ± 0.268
4.753 ± 0.26
3.315 ± 0.426
7.942 ± 0.575
2.752 ± 0.393
4.065 ± 0.131
3.064 ± 0.341
8.568 ± 1.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.689 ± 0.349
4.315 ± 0.046
5.754 ± 0.664
2.376 ± 0.141
5.879 ± 0.598
7.567 ± 0.065
6.942 ± 0.463
7.692 ± 0.75
1.188 ± 0.02
3.627 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here