Streptococcus phage CHPC1046
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8F9Z1|A0A3G8F9Z1_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1046 OX=2365018 GN=CHPC1046_0043 PE=4 SV=1
MM1 pKa = 7.11 VNWVDD6 pKa = 4.56 ADD8 pKa = 4.01 GNDD11 pKa = 3.57 IPDD14 pKa = 4.65 GADD17 pKa = 2.92 QDD19 pKa = 4.82 FKK21 pKa = 11.67 AGMYY25 pKa = 10.19 FSFAGDD31 pKa = 3.52 EE32 pKa = 4.3 VNITDD37 pKa = 3.77 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.854
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.427
ProMoST 3.859
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.656
Rodwell 3.478
Grimsley 3.338
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.77
Patrickios 0.095
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.727
Protein with the highest isoelectric point:
>tr|A0A3G8FA08|A0A3G8FA08_9CAUD Antirepressor protein OS=Streptococcus phage CHPC1046 OX=2365018 GN=CHPC1046_0028 PE=4 SV=1
MM1 pKa = 7.8 DD2 pKa = 5.25 AEE4 pKa = 4.48 GLSFHH9 pKa = 7.42 IYY11 pKa = 10.71 HH12 pKa = 5.85 NTKK15 pKa = 8.94 KK16 pKa = 10.45 QKK18 pKa = 10.64 GVLTPNKK25 pKa = 8.6 MFPFSRR31 pKa = 11.84 DD32 pKa = 3.06 LNLISLSAFAAFALDD47 pKa = 4.1 KK48 pKa = 10.91 LYY50 pKa = 10.93 LGYY53 pKa = 9.79 PITFYY58 pKa = 11.02 KK59 pKa = 10.21 IIIYY63 pKa = 10.07 KK64 pKa = 9.69 SNKK67 pKa = 8.98 KK68 pKa = 10.32 SPSKK72 pKa = 9.96 MLRR75 pKa = 11.84 LRR77 pKa = 11.84 PLLPWYY83 pKa = 8.28 PYY85 pKa = 10.25 CSVRR89 pKa = 11.84 GGDD92 pKa = 3.03 ILLFILFLLVVYY104 pKa = 8.48 WW105 pKa = 3.63
Molecular weight: 12.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.148
IPC2_protein 9.487
IPC_protein 9.428
Toseland 9.838
ProMoST 9.619
Dawson 10.116
Bjellqvist 9.809
Wikipedia 10.306
Rodwell 10.482
Grimsley 10.204
Solomon 10.145
Lehninger 10.101
Nozaki 9.853
DTASelect 9.809
Thurlkill 9.94
EMBOSS 10.262
Sillero 10.028
Patrickios 7.497
IPC_peptide 10.131
IPC2_peptide 8.478
IPC2.peptide.svr19 8.434
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
10679
44
1593
232.2
26.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.358 ± 0.482
0.524 ± 0.121
6.386 ± 0.238
6.958 ± 0.628
4.289 ± 0.193
6.33 ± 0.521
1.517 ± 0.093
6.574 ± 0.233
8.54 ± 0.5
8.1 ± 0.405
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.172
6.386 ± 0.308
2.959 ± 0.167
4.523 ± 0.266
3.942 ± 0.261
6.443 ± 0.266
6.293 ± 0.374
5.881 ± 0.259
1.48 ± 0.161
4.045 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here