Lacrimispora algidixylanolytica
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4081 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A419T2V3|A0A419T2V3_9FIRM Glyco_hydro_18 domain-containing protein OS=Lacrimispora algidixylanolytica OX=94868 GN=BET01_19650 PE=4 SV=1
MM1 pKa = 7.37 QDD3 pKa = 3.37 YY4 pKa = 10.29 FPVVVQVIPLDD15 pKa = 3.84 NYY17 pKa = 9.3 HH18 pKa = 5.84 VQVFFDD24 pKa = 3.15 NGKK27 pKa = 9.31 IVEE30 pKa = 4.25 YY31 pKa = 10.32 DD32 pKa = 3.34 ASSLLQGEE40 pKa = 4.62 VFKK43 pKa = 10.46 PLQDD47 pKa = 3.05 IQVFKK52 pKa = 10.14 DD53 pKa = 3.06 TCTVLNDD60 pKa = 3.45 TLAWDD65 pKa = 3.63 ISGDD69 pKa = 3.56 RR70 pKa = 11.84 SLSEE74 pKa = 5.71 CIDD77 pKa = 4.11 LDD79 pKa = 3.64 PFMLYY84 pKa = 10.03 EE85 pKa = 4.35 LEE87 pKa = 4.77 SINHH91 pKa = 7.08 LIAA94 pKa = 5.54
Molecular weight: 10.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 0.846
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A419TCY5|A0A419TCY5_9FIRM Ribosomal RNA small subunit methyltransferase G OS=Lacrimispora algidixylanolytica OX=94868 GN=rsmG PE=3 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.95 KK9 pKa = 7.58 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.17 VHH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTPGGRR28 pKa = 11.84 KK29 pKa = 8.8 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.33 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 4.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4081
0
4081
1335621
29
5189
327.3
36.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.76 ± 0.04
1.34 ± 0.016
5.444 ± 0.025
7.282 ± 0.041
4.306 ± 0.032
7.25 ± 0.044
1.755 ± 0.016
7.781 ± 0.041
6.959 ± 0.032
9.28 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.079 ± 0.021
4.604 ± 0.03
3.369 ± 0.026
3.157 ± 0.021
3.995 ± 0.026
6.414 ± 0.028
5.475 ± 0.031
6.663 ± 0.029
0.96 ± 0.014
4.13 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here