Amino acid dipepetide frequency for Salmo trutta (Brown trout)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.263AlaAla: 5.263 ± 0.025	1.294AlaCys: 1.294 ± 0.005	2.962AlaAsp: 2.962 ± 0.007	4.194AlaGlu: 4.194 ± 0.012	2.327AlaPhe: 2.327 ± 0.006	4.13AlaGly: 4.13 ± 0.01	1.44AlaHis: 1.44 ± 0.005	2.797AlaIle: 2.797 ± 0.007	3.302AlaLys: 3.302 ± 0.009	6.323AlaLeu: 6.323 ± 0.011	1.684AlaMet: 1.684 ± 0.004	2.187AlaAsn: 2.187 ± 0.006	3.27AlaPro: 3.27 ± 0.011	2.839AlaGln: 2.839 ± 0.008	3.051AlaArg: 3.051 ± 0.009	4.998AlaSer: 4.998 ± 0.011	3.456AlaThr: 3.456 ± 0.01	4.736AlaVal: 4.736 ± 0.011	0.651AlaTrp: 0.651 ± 0.003	1.549AlaTyr: 1.549 ± 0.004	0.0AlaXaa: 0.0 ± 0.0
Cys	1.154CysAla: 1.154 ± 0.005	0.651CysCys: 0.651 ± 0.005	1.221CysAsp: 1.221 ± 0.007	1.248CysGlu: 1.248 ± 0.007	0.915CysPhe: 0.915 ± 0.004	1.764CysGly: 1.764 ± 0.009	0.67CysHis: 0.67 ± 0.004	1.043CysIle: 1.043 ± 0.005	1.131CysLys: 1.131 ± 0.005	2.324CysLeu: 2.324 ± 0.01	0.496CysMet: 0.496 ± 0.003	0.867CysAsn: 0.867 ± 0.004	1.32CysPro: 1.32 ± 0.008	1.049CysGln: 1.049 ± 0.006	1.258CysArg: 1.258 ± 0.006	2.153CysSer: 2.153 ± 0.009	1.246CysThr: 1.246 ± 0.006	1.814CysVal: 1.814 ± 0.011	0.3CysTrp: 0.3 ± 0.002	0.651CysTyr: 0.651 ± 0.004	0.0CysXaa: 0.0 ± 0.0
Asp	2.759AspAla: 2.759 ± 0.007	1.226AspCys: 1.226 ± 0.007	2.99AspAsp: 2.99 ± 0.008	3.51AspGlu: 3.51 ± 0.009	2.094AspPhe: 2.094 ± 0.006	3.578AspGly: 3.578 ± 0.01	1.271AspHis: 1.271 ± 0.005	2.792AspIle: 2.792 ± 0.007	2.771AspLys: 2.771 ± 0.008	5.069AspLeu: 5.069 ± 0.011	1.4AspMet: 1.4 ± 0.005	2.084AspAsn: 2.084 ± 0.007	2.946AspPro: 2.946 ± 0.007	2.119AspGln: 2.119 ± 0.006	2.777AspArg: 2.777 ± 0.008	4.218AspSer: 4.218 ± 0.011	2.798AspThr: 2.798 ± 0.008	3.269AspVal: 3.269 ± 0.008	0.71AspTrp: 0.71 ± 0.003	1.627AspTyr: 1.627 ± 0.005	0.0AspXaa: 0.0 ± 0.0
Glu	4.475GluAla: 4.475 ± 0.012	1.295GluCys: 1.295 ± 0.008	4.301GluAsp: 4.301 ± 0.009	7.623GluGlu: 7.623 ± 0.027	1.955GluPhe: 1.955 ± 0.006	4.502GluGly: 4.502 ± 0.011	1.467GluHis: 1.467 ± 0.005	2.787GluIle: 2.787 ± 0.007	4.679GluLys: 4.679 ± 0.015	6.089GluLeu: 6.089 ± 0.016	1.808GluMet: 1.808 ± 0.007	2.607GluAsn: 2.607 ± 0.007	2.685GluPro: 2.685 ± 0.007	2.934GluGln: 2.934 ± 0.01	4.389GluArg: 4.389 ± 0.018	4.059GluSer: 4.059 ± 0.01	3.395GluThr: 3.395 ± 0.012	4.456GluVal: 4.456 ± 0.01	0.714GluTrp: 0.714 ± 0.003	1.647GluTyr: 1.647 ± 0.005	0.0GluXaa: 0.0 ± 0.0
Phe	1.87PheAla: 1.87 ± 0.005	0.938PheCys: 0.938 ± 0.005	1.8PheAsp: 1.8 ± 0.006	1.873PheGlu: 1.873 ± 0.006	1.56PhePhe: 1.56 ± 0.006	2.225PheGly: 2.225 ± 0.007	1.052PheHis: 1.052 ± 0.004	1.922PheIle: 1.922 ± 0.006	1.798PheLys: 1.798 ± 0.006	3.894PheLeu: 3.894 ± 0.01	0.837PheMet: 0.837 ± 0.004	1.574PheAsn: 1.574 ± 0.005	1.81PhePro: 1.81 ± 0.006	1.68PheGln: 1.68 ± 0.006	1.866PheArg: 1.866 ± 0.005	3.381PheSer: 3.381 ± 0.007	2.366PheThr: 2.366 ± 0.007	2.21PheVal: 2.21 ± 0.006	0.468PheTrp: 0.468 ± 0.003	1.242PheTyr: 1.242 ± 0.005	0.0PheXaa: 0.0 ± 0.0
Gly	3.938GlyAla: 3.938 ± 0.011	1.315GlyCys: 1.315 ± 0.007	3.398GlyAsp: 3.398 ± 0.009	4.504GlyGlu: 4.504 ± 0.011	2.422GlyPhe: 2.422 ± 0.008	5.608GlyGly: 5.608 ± 0.018	1.763GlyHis: 1.763 ± 0.006	2.653GlyIle: 2.653 ± 0.007	3.851GlyLys: 3.851 ± 0.009	5.652GlyLeu: 5.652 ± 0.012	1.657GlyMet: 1.657 ± 0.006	2.528GlyAsn: 2.528 ± 0.009	3.347GlyPro: 3.347 ± 0.016	2.947GlyGln: 2.947 ± 0.009	3.655GlyArg: 3.655 ± 0.01	5.542GlySer: 5.542 ± 0.014	3.561GlyThr: 3.561 ± 0.01	4.19GlyVal: 4.19 ± 0.01	0.801GlyTrp: 0.801 ± 0.004	1.979GlyTyr: 1.979 ± 0.007	0.0GlyXaa: 0.0 ± 0.0
His	1.314HisAla: 1.314 ± 0.005	0.877HisCys: 0.877 ± 0.005	1.021HisAsp: 1.021 ± 0.004	1.23HisGlu: 1.23 ± 0.005	1.053HisPhe: 1.053 ± 0.004	1.626HisGly: 1.626 ± 0.007	1.09HisHis: 1.09 ± 0.007	1.379HisIle: 1.379 ± 0.005	1.281HisLys: 1.281 ± 0.005	2.785HisLeu: 2.785 ± 0.008	0.667HisMet: 0.667 ± 0.004	1.072HisAsn: 1.072 ± 0.005	1.646HisPro: 1.646 ± 0.007	1.442HisGln: 1.442 ± 0.007	1.632HisArg: 1.632 ± 0.005	2.451HisSer: 2.451 ± 0.008	2.175HisThr: 2.175 ± 0.02	1.464HisVal: 1.464 ± 0.005	0.347HisTrp: 0.347 ± 0.002	0.917HisTyr: 0.917 ± 0.005	0.0HisXaa: 0.0 ± 0.0
Ile	2.566IleAla: 2.566 ± 0.007	1.053IleCys: 1.053 ± 0.004	2.216IleAsp: 2.216 ± 0.006	2.448IleGlu: 2.448 ± 0.008	1.795IlePhe: 1.795 ± 0.006	2.408IleGly: 2.408 ± 0.007	1.44IleHis: 1.44 ± 0.007	2.425IleIle: 2.425 ± 0.007	2.548IleLys: 2.548 ± 0.007	4.391IleLeu: 4.391 ± 0.011	1.128IleMet: 1.128 ± 0.004	2.008IleAsn: 2.008 ± 0.006	2.495IlePro: 2.495 ± 0.008	2.207IleGln: 2.207 ± 0.007	2.435IleArg: 2.435 ± 0.006	3.621IleSer: 3.621 ± 0.009	2.891IleThr: 2.891 ± 0.008	2.693IleVal: 2.693 ± 0.008	0.472IleTrp: 0.472 ± 0.003	1.408IleTyr: 1.408 ± 0.005	0.0IleXaa: 0.0 ± 0.0
Lys	3.648LysAla: 3.648 ± 0.008	1.03LysCys: 1.03 ± 0.006	3.192LysAsp: 3.192 ± 0.008	4.623LysGlu: 4.623 ± 0.013	1.613LysPhe: 1.613 ± 0.006	3.271LysGly: 3.271 ± 0.009	1.455LysHis: 1.455 ± 0.005	2.466LysIle: 2.466 ± 0.007	4.058LysLys: 4.058 ± 0.013	4.89LysLeu: 4.89 ± 0.012	1.494LysMet: 1.494 ± 0.006	2.191LysAsn: 2.191 ± 0.007	2.914LysPro: 2.914 ± 0.01	2.488LysGln: 2.488 ± 0.008	3.332LysArg: 3.332 ± 0.008	3.731LysSer: 3.731 ± 0.01	3.148LysThr: 3.148 ± 0.007	3.586LysVal: 3.586 ± 0.009	0.588LysTrp: 0.588 ± 0.003	1.534LysTyr: 1.534 ± 0.005	0.0LysXaa: 0.0 ± 0.0
Leu	6.042LeuAla: 6.042 ± 0.013	2.308LeuCys: 2.308 ± 0.007	4.974LeuAsp: 4.974 ± 0.011	6.451LeuGlu: 6.451 ± 0.018	3.543LeuPhe: 3.543 ± 0.01	5.442LeuGly: 5.442 ± 0.01	2.771LeuHis: 2.771 ± 0.009	3.86LeuIle: 3.86 ± 0.009	5.562LeuLys: 5.562 ± 0.013	9.977LeuLeu: 9.977 ± 0.021	2.153LeuMet: 2.153 ± 0.007	3.675LeuAsn: 3.675 ± 0.008	5.298LeuPro: 5.298 ± 0.012	5.262LeuGln: 5.262 ± 0.016	5.557LeuArg: 5.557 ± 0.011	8.609LeuSer: 8.609 ± 0.022	5.479LeuThr: 5.479 ± 0.012	5.645LeuVal: 5.645 ± 0.011	1.068LeuTrp: 1.068 ± 0.004	2.774LeuTyr: 2.774 ± 0.008	0.0LeuXaa: 0.0 ± 0.0
Met	1.977MetAla: 1.977 ± 0.006	0.527MetCys: 0.527 ± 0.003	1.557MetAsp: 1.557 ± 0.005	2.096MetGlu: 2.096 ± 0.006	0.902MetPhe: 0.902 ± 0.004	1.607MetGly: 1.607 ± 0.006	0.524MetHis: 0.524 ± 0.003	0.904MetIle: 0.904 ± 0.003	1.484MetLys: 1.484 ± 0.005	2.146MetLeu: 2.146 ± 0.005	0.729MetMet: 0.729 ± 0.004	0.94MetAsn: 0.94 ± 0.004	1.135MetPro: 1.135 ± 0.006	1.025MetGln: 1.025 ± 0.004	1.226MetArg: 1.226 ± 0.005	1.888MetSer: 1.888 ± 0.005	1.334MetThr: 1.334 ± 0.005	1.733MetVal: 1.733 ± 0.005	0.27MetTrp: 0.27 ± 0.002	0.696MetTyr: 0.696 ± 0.003	0.0MetXaa: 0.0 ± 0.0
Asn	2.12AsnAla: 2.12 ± 0.006	0.884AsnCys: 0.884 ± 0.005	1.771AsnAsp: 1.771 ± 0.007	2.062AsnGlu: 2.062 ± 0.006	1.382AsnPhe: 1.382 ± 0.005	2.832AsnGly: 2.832 ± 0.011	1.057AsnHis: 1.057 ± 0.004	2.178AsnIle: 2.178 ± 0.007	2.19AsnLys: 2.19 ± 0.006	3.703AsnLeu: 3.703 ± 0.01	1.106AsnMet: 1.106 ± 0.004	1.844AsnAsn: 1.844 ± 0.007	2.339AsnPro: 2.339 ± 0.008	1.756AsnGln: 1.756 ± 0.006	1.994AsnArg: 1.994 ± 0.006	3.081AsnSer: 3.081 ± 0.008	2.419AsnThr: 2.419 ± 0.008	2.352AsnVal: 2.352 ± 0.006	0.453AsnTrp: 0.453 ± 0.003	1.196AsnTyr: 1.196 ± 0.005	0.0AsnXaa: 0.0 ± 0.0
Pro	3.738ProAla: 3.738 ± 0.013	1.109ProCys: 1.109 ± 0.007	2.856ProAsp: 2.856 ± 0.008	3.67ProGlu: 3.67 ± 0.008	1.82ProPhe: 1.82 ± 0.006	4.118ProGly: 4.118 ± 0.02	1.564ProHis: 1.564 ± 0.007	2.021ProIle: 2.021 ± 0.007	2.447ProLys: 2.447 ± 0.01	4.988ProLeu: 4.988 ± 0.013	1.143ProMet: 1.143 ± 0.005	1.976ProAsn: 1.976 ± 0.008	5.141ProPro: 5.141 ± 0.023	2.654ProGln: 2.654 ± 0.011	2.678ProArg: 2.678 ± 0.008	5.412ProSer: 5.412 ± 0.016	3.249ProThr: 3.249 ± 0.014	3.672ProVal: 3.672 ± 0.01	0.564ProTrp: 0.564 ± 0.003	1.606ProTyr: 1.606 ± 0.006	0.0ProXaa: 0.0 ± 0.0
Gln	3.204GlnAla: 3.204 ± 0.01	1.218GlnCys: 1.218 ± 0.007	2.346GlnAsp: 2.346 ± 0.007	3.403GlnGlu: 3.403 ± 0.01	1.395GlnPhe: 1.395 ± 0.004	2.998GlnGly: 2.998 ± 0.009	1.385GlnHis: 1.385 ± 0.005	1.907GlnIle: 1.907 ± 0.005	2.413GlnLys: 2.413 ± 0.008	4.446GlnLeu: 4.446 ± 0.011	1.137GlnMet: 1.137 ± 0.005	1.687GlnAsn: 1.687 ± 0.006	2.535GlnPro: 2.535 ± 0.01	3.165GlnGln: 3.165 ± 0.018	3.135GlnArg: 3.135 ± 0.009	3.348GlnSer: 3.348 ± 0.009	2.542GlnThr: 2.542 ± 0.01	2.882GlnVal: 2.882 ± 0.008	0.563GlnTrp: 0.563 ± 0.003	1.32GlnTyr: 1.32 ± 0.005	0.0GlnXaa: 0.0 ± 0.0
Arg	3.297ArgAla: 3.297 ± 0.009	1.236ArgCys: 1.236 ± 0.007	2.998ArgAsp: 2.998 ± 0.008	4.005ArgGlu: 4.005 ± 0.017	1.957ArgPhe: 1.957 ± 0.006	3.527ArgGly: 3.527 ± 0.011	1.564ArgHis: 1.564 ± 0.006	2.438ArgIle: 2.438 ± 0.007	3.385ArgLys: 3.385 ± 0.008	5.246ArgLeu: 5.246 ± 0.012	1.355ArgMet: 1.355 ± 0.005	2.132ArgAsn: 2.132 ± 0.006	2.917ArgPro: 2.917 ± 0.008	2.641ArgGln: 2.641 ± 0.008	4.003ArgArg: 4.003 ± 0.01	4.227ArgSer: 4.227 ± 0.011	3.009ArgThr: 3.009 ± 0.008	3.387ArgVal: 3.387 ± 0.009	0.668ArgTrp: 0.668 ± 0.004	1.588ArgTyr: 1.588 ± 0.005	0.0ArgXaa: 0.0 ± 0.0
Ser	4.943SerAla: 4.943 ± 0.011	1.962SerCys: 1.962 ± 0.009	4.088SerAsp: 4.088 ± 0.009	4.586SerGlu: 4.586 ± 0.011	3.14SerPhe: 3.14 ± 0.009	5.469SerGly: 5.469 ± 0.012	2.296SerHis: 2.296 ± 0.008	3.39SerIle: 3.39 ± 0.009	3.737SerLys: 3.737 ± 0.009	8.64SerLeu: 8.64 ± 0.023	1.9SerMet: 1.9 ± 0.006	2.97SerAsn: 2.97 ± 0.008	5.771SerPro: 5.771 ± 0.02	3.768SerGln: 3.768 ± 0.01	4.183SerArg: 4.183 ± 0.01	8.966SerSer: 8.966 ± 0.026	4.759SerThr: 4.759 ± 0.012	5.349SerVal: 5.349 ± 0.014	0.944SerTrp: 0.944 ± 0.004	2.202SerTyr: 2.202 ± 0.007	0.0SerXaa: 0.0 ± 0.0
Thr	3.903ThrAla: 3.903 ± 0.013	1.379ThrCys: 1.379 ± 0.008	2.929ThrAsp: 2.929 ± 0.009	3.745ThrGlu: 3.745 ± 0.011	2.131ThrPhe: 2.131 ± 0.006	4.122ThrGly: 4.122 ± 0.015	1.892ThrHis: 1.892 ± 0.02	2.564ThrIle: 2.564 ± 0.008	2.692ThrLys: 2.692 ± 0.007	5.656ThrLeu: 5.656 ± 0.01	1.344ThrMet: 1.344 ± 0.004	1.985ThrAsn: 1.985 ± 0.005	3.765ThrPro: 3.765 ± 0.016	2.499ThrGln: 2.499 ± 0.007	2.585ThrArg: 2.585 ± 0.006	4.704ThrSer: 4.704 ± 0.013	3.737ThrThr: 3.737 ± 0.033	4.357ThrVal: 4.357 ± 0.011	0.678ThrTrp: 0.678 ± 0.004	1.501ThrTyr: 1.501 ± 0.005	0.0ThrXaa: 0.0 ± 0.0
Val	4.102ValAla: 4.102 ± 0.009	1.932ValCys: 1.932 ± 0.009	3.285ValAsp: 3.285 ± 0.008	4.209ValGlu: 4.209 ± 0.012	2.7ValPhe: 2.7 ± 0.007	3.645ValGly: 3.645 ± 0.009	1.64ValHis: 1.64 ± 0.005	3.042ValIle: 3.042 ± 0.008	3.717ValLys: 3.717 ± 0.008	6.262ValLeu: 6.262 ± 0.013	1.586ValMet: 1.586 ± 0.005	2.586ValAsn: 2.586 ± 0.007	3.271ValPro: 3.271 ± 0.009	2.742ValGln: 2.742 ± 0.008	3.348ValArg: 3.348 ± 0.009	5.25ValSer: 5.25 ± 0.011	4.118ValThr: 4.118 ± 0.014	4.563ValVal: 4.563 ± 0.01	0.803ValTrp: 0.803 ± 0.003	1.94ValTyr: 1.94 ± 0.006	0.0ValXaa: 0.0 ± 0.0
Trp	0.683TrpAla: 0.683 ± 0.004	0.268TrpCys: 0.268 ± 0.003	0.665TrpAsp: 0.665 ± 0.003	0.753TrpGlu: 0.753 ± 0.004	0.443TrpPhe: 0.443 ± 0.002	0.656TrpGly: 0.656 ± 0.004	0.28TrpHis: 0.28 ± 0.002	0.546TrpIle: 0.546 ± 0.004	0.703TrpLys: 0.703 ± 0.004	1.157TrpLeu: 1.157 ± 0.005	0.359TrpMet: 0.359 ± 0.003	0.537TrpAsn: 0.537 ± 0.003	0.453TrpPro: 0.453 ± 0.003	0.498TrpGln: 0.498 ± 0.003	0.739TrpArg: 0.739 ± 0.004	0.945TrpSer: 0.945 ± 0.005	0.697TrpThr: 0.697 ± 0.004	0.727TrpVal: 0.727 ± 0.004	0.204TrpTrp: 0.204 ± 0.002	0.354TrpTyr: 0.354 ± 0.002	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.457TyrAla: 1.457 ± 0.005	0.772TyrCys: 0.772 ± 0.004	1.436TyrAsp: 1.436 ± 0.005	1.631TyrGlu: 1.631 ± 0.006	1.218TyrPhe: 1.218 ± 0.004	1.784TyrGly: 1.784 ± 0.007	0.853TyrHis: 0.853 ± 0.004	1.534TyrIle: 1.534 ± 0.006	1.504TyrLys: 1.504 ± 0.006	2.812TyrLeu: 2.812 ± 0.007	0.717TyrMet: 0.717 ± 0.003	1.268TyrAsn: 1.268 ± 0.005	1.369TyrPro: 1.369 ± 0.006	1.316TyrGln: 1.316 ± 0.004	1.736TyrArg: 1.736 ± 0.006	2.478TyrSer: 2.478 ± 0.007	1.787TyrThr: 1.787 ± 0.007	1.659TyrVal: 1.659 ± 0.007	0.401TyrTrp: 0.401 ± 0.003	1.018TyrTyr: 1.018 ± 0.005	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.049XaaXaa: 0.049 ± 0.005

Statistics based on 114752 proteins (79092270 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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