Lactococcus phage CaseusJM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus CaseusJM1

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1IDC9|M1IDC9_9CAUD Structural protein OS=Lactococcus phage CaseusJM1 OX=1289467 PE=4 SV=1
MM1 pKa = 7.56NEE3 pKa = 3.62FDD5 pKa = 6.3SYY7 pKa = 11.39IDD9 pKa = 3.33WYY11 pKa = 11.58NNLLTMPLNDD21 pKa = 3.86VILGVKK27 pKa = 8.96DD28 pKa = 3.57TIEE31 pKa = 4.55DD32 pKa = 3.52KK33 pKa = 10.66TVYY36 pKa = 10.62LSLSDD41 pKa = 3.95SKK43 pKa = 10.99VLKK46 pKa = 9.75MDD48 pKa = 3.09NTSFVMGYY56 pKa = 9.13YY57 pKa = 8.74YY58 pKa = 10.45QVVLSVKK65 pKa = 10.52DD66 pKa = 3.51VDD68 pKa = 4.41DD69 pKa = 4.44EE70 pKa = 4.64LVGLVGNVLQNGWNMTNWSEE90 pKa = 4.32NSHH93 pKa = 6.58LYY95 pKa = 10.7NYY97 pKa = 8.61TGTVYY102 pKa = 10.42LPCGAGGQAWQQ113 pKa = 3.59

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1HMF5|M1HMF5_9CAUD Uncharacterized protein OS=Lactococcus phage CaseusJM1 OX=1289467 PE=4 SV=1
MM1 pKa = 7.47ISNIYY6 pKa = 8.06FHH8 pKa = 7.44KK9 pKa = 10.7EE10 pKa = 3.58NIVNNMCKK18 pKa = 9.73KK19 pKa = 10.33RR20 pKa = 11.84KK21 pKa = 5.77YY22 pKa = 8.39TKK24 pKa = 9.78MGALYY29 pKa = 10.54SIVNAQHH36 pKa = 6.57RR37 pKa = 11.84KK38 pKa = 9.53KK39 pKa = 10.44KK40 pKa = 10.08ADD42 pKa = 4.13KK43 pKa = 9.8IPVRR47 pKa = 11.84AYY49 pKa = 9.48HH50 pKa = 6.61CKK52 pKa = 8.93WCNLYY57 pKa = 10.43HH58 pKa = 7.32LSSQQRR64 pKa = 11.84LNIKK68 pKa = 9.21TGVIGG73 pKa = 3.97

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

9512

36

916

186.5

21.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.161 ± 0.648

0.631 ± 0.143

5.898 ± 0.358

7.517 ± 0.607

4.195 ± 0.312

6.266 ± 0.683

1.304 ± 0.17

7.222 ± 0.311

9.094 ± 0.5

8.547 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.141

6.833 ± 0.268

2.176 ± 0.241

3.522 ± 0.213

3.438 ± 0.292

6.444 ± 0.516

6.476 ± 0.37

6.36 ± 0.429

1.377 ± 0.155

4.048 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski