Clostridium sp. CAG:302

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1248 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6MCN7|R6MCN7_9CLOT Thymidylate kinase OS=Clostridium sp. CAG:302 OX=1262793 GN=BN595_00856 PE=4 SV=1
MM1 pKa = 7.12QNYY4 pKa = 9.33NVVPNIISGKK14 pKa = 10.1DD15 pKa = 3.3LDD17 pKa = 4.34YY18 pKa = 11.36LSDD21 pKa = 3.65MFNWNYY27 pKa = 10.1TGYY30 pKa = 10.71KK31 pKa = 9.3EE32 pKa = 5.49LYY34 pKa = 7.7NTLEE38 pKa = 4.46NISDD42 pKa = 4.03NEE44 pKa = 4.23VKK46 pKa = 10.26TMISRR51 pKa = 11.84VSEE54 pKa = 3.81VLYY57 pKa = 11.4NNMTNILNVLGGTYY71 pKa = 9.11EE72 pKa = 3.81

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6M837|R6M837_9CLOT Spore surface glycoprotein BclB OS=Clostridium sp. CAG:302 OX=1262793 GN=BN595_00929 PE=4 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84KK4 pKa = 10.08SMIVKK9 pKa = 9.8SEE11 pKa = 4.07RR12 pKa = 11.84EE13 pKa = 3.84PKK15 pKa = 10.42FKK17 pKa = 10.34VRR19 pKa = 11.84KK20 pKa = 6.0YY21 pKa = 8.4TRR23 pKa = 11.84CKK25 pKa = 9.34ICGRR29 pKa = 11.84PHH31 pKa = 7.12AVLKK35 pKa = 10.69KK36 pKa = 10.44YY37 pKa = 9.45GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.62KK58 pKa = 10.92SSWW61 pKa = 3.1

Molecular weight: 7.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1248

0

1248

363869

30

1863

291.6

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.936 ± 0.071

1.009 ± 0.028

6.259 ± 0.056

7.081 ± 0.075

4.082 ± 0.055

5.313 ± 0.075

1.169 ± 0.028

10.513 ± 0.092

9.955 ± 0.07

8.983 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.035

7.956 ± 0.084

2.401 ± 0.035

1.601 ± 0.035

3.16 ± 0.049

6.456 ± 0.057

5.691 ± 0.071

5.989 ± 0.048

0.52 ± 0.019

5.545 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski