Tamus red mosaic virus
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G3LHV5|G3LHV5_9VIRU Tgb1 OS=Tamus red mosaic virus OX=1081702 PE=4 SV=1
MM1 pKa = 7.3 SRR3 pKa = 11.84 LRR5 pKa = 11.84 GALEE9 pKa = 3.9 QFTDD13 pKa = 3.93 PAQKK17 pKa = 10.15 IVIQNEE23 pKa = 4.32 QYY25 pKa = 9.54 QHH27 pKa = 5.35 LRR29 pKa = 11.84 KK30 pKa = 7.83 TLSHH34 pKa = 6.58 TATTNPYY41 pKa = 10.4 AIAEE45 pKa = 4.28 SAANVLEE52 pKa = 4.19 DD53 pKa = 5.01 LGINSNPFATTAHH66 pKa = 5.31 THH68 pKa = 5.42 SAAKK72 pKa = 10.35 AIEE75 pKa = 3.91 NDD77 pKa = 3.82 LYY79 pKa = 11.25 FSASSLLPKK88 pKa = 10.03 EE89 pKa = 4.37 NPVSFLFMKK98 pKa = 9.83 PAKK101 pKa = 10.18 LRR103 pKa = 11.84 FFHH106 pKa = 7.34 RR107 pKa = 11.84 GPQHH111 pKa = 6.45 NDD113 pKa = 2.24 HH114 pKa = 7.32 FINKK118 pKa = 8.22 VVEE121 pKa = 4.44 PKK123 pKa = 10.6 DD124 pKa = 3.51 VARR127 pKa = 11.84 YY128 pKa = 10.1 DD129 pKa = 3.71 PDD131 pKa = 3.84 TLVSSLPTLSTSHH144 pKa = 7.23 AFMGDD149 pKa = 3.22 SLHH152 pKa = 6.5 FLPFSFLVRR161 pKa = 11.84 LFSDD165 pKa = 3.71 NVQLKK170 pKa = 8.08 TLVATMVLPAEE181 pKa = 4.46 AKK183 pKa = 10.26 FNLASIYY190 pKa = 10.18 PNIYY194 pKa = 7.66 TLQYY198 pKa = 10.01 HH199 pKa = 5.85 RR200 pKa = 11.84 CKK202 pKa = 10.18 KK203 pKa = 10.63 GKK205 pKa = 8.13 VTHH208 pKa = 6.66 FSYY211 pKa = 11.18 APGGHH216 pKa = 6.66 KK217 pKa = 10.13 GAEE220 pKa = 4.14 YY221 pKa = 10.53 VHH223 pKa = 6.38 EE224 pKa = 4.7 LKK226 pKa = 10.74 QLDD229 pKa = 3.83 WLSVGRR235 pKa = 11.84 LTDD238 pKa = 3.58 GVTTITAQKK247 pKa = 10.26 IEE249 pKa = 4.36 TKK251 pKa = 10.32 AANHH255 pKa = 6.15 LFIFQRR261 pKa = 11.84 GSLMTPCLRR270 pKa = 11.84 NFSTEE275 pKa = 4.08 TKK277 pKa = 10.21 FVTLPLIFLPEE288 pKa = 4.11 KK289 pKa = 10.49 FNARR293 pKa = 11.84 QPIRR297 pKa = 11.84 KK298 pKa = 6.84 TLVMQMFLYY307 pKa = 10.66 VKK309 pKa = 10.02 GVKK312 pKa = 9.25 QVSEE316 pKa = 3.94 RR317 pKa = 11.84 DD318 pKa = 2.79 IWAKK322 pKa = 8.77 WRR324 pKa = 11.84 QLIKK328 pKa = 10.22 TEE330 pKa = 4.12 DD331 pKa = 3.73 LQDD334 pKa = 4.28 FSAIEE339 pKa = 4.02 LLHH342 pKa = 5.39 LTNYY346 pKa = 9.75 FVFVGKK352 pKa = 10.23 QSSINCFDD360 pKa = 4.39 DD361 pKa = 3.52 VLKK364 pKa = 11.2 GGFLTRR370 pKa = 11.84 WFKK373 pKa = 10.54 PIKK376 pKa = 9.87 NWFEE380 pKa = 3.82 MVRR383 pKa = 11.84 QKK385 pKa = 10.84 IFGEE389 pKa = 4.27 AEE391 pKa = 3.9 FVRR394 pKa = 11.84 LVRR397 pKa = 11.84 ALEE400 pKa = 3.97 WTEE403 pKa = 3.68 VDD405 pKa = 5.29 LKK407 pKa = 11.31 LDD409 pKa = 3.48 TKK411 pKa = 11.24 NYY413 pKa = 9.53 DD414 pKa = 3.35 FKK416 pKa = 11.13 RR417 pKa = 11.84 RR418 pKa = 11.84 EE419 pKa = 4.12 GNGFLSLSKK428 pKa = 10.66 VAAGPDD434 pKa = 3.31 DD435 pKa = 4.58 SEE437 pKa = 5.6 DD438 pKa = 3.74 VFHH441 pKa = 7.3 LPQEE445 pKa = 4.26 EE446 pKa = 4.85 SLFSQMEE453 pKa = 4.03 WLGEE457 pKa = 3.6 FWNFIKK463 pKa = 10.96 GEE465 pKa = 4.06 NGKK468 pKa = 9.61 KK469 pKa = 9.63 VAEE472 pKa = 4.28 PCPNEE477 pKa = 4.18 PVCEE481 pKa = 4.16 TNVDD485 pKa = 3.71 EE486 pKa = 5.31 DD487 pKa = 3.85 QSAHH491 pKa = 4.74 EE492 pKa = 4.29 WLLKK496 pKa = 10.54 LEE498 pKa = 4.07 QTRR501 pKa = 11.84 VVEE504 pKa = 4.38 EE505 pKa = 4.47 TPLEE509 pKa = 4.14 QPTEE513 pKa = 3.99 VSTTLDD519 pKa = 3.03 ATLTMLPDD527 pKa = 3.74 NSQMSEE533 pKa = 4.03 DD534 pKa = 3.71 EE535 pKa = 4.2 KK536 pKa = 11.47 VVAEE540 pKa = 4.18 GAITEE545 pKa = 4.35 SEE547 pKa = 4.06 QAPEE551 pKa = 3.96 EE552 pKa = 4.14 KK553 pKa = 10.51 AEE555 pKa = 4.16 EE556 pKa = 4.37 LEE558 pKa = 4.39 QVDD561 pKa = 4.35 SSPLQAEE568 pKa = 4.48 VAQIRR573 pKa = 11.84 FGTAPTKK580 pKa = 11.05 AEE582 pKa = 3.81 VAALIRR588 pKa = 11.84 TAMKK592 pKa = 9.97 QKK594 pKa = 10.47 QISLEE599 pKa = 4.01 PLEE602 pKa = 4.45 ATAGCEE608 pKa = 3.82 QEE610 pKa = 4.15 MMEE613 pKa = 4.74 GSSSAAVLEE622 pKa = 4.88 DD623 pKa = 4.1 PPATTSDD630 pKa = 3.62 DD631 pKa = 3.73 TAEE634 pKa = 4.8 DD635 pKa = 4.15 DD636 pKa = 4.71 SGLTSDD642 pKa = 3.69 EE643 pKa = 4.65 RR644 pKa = 11.84 EE645 pKa = 3.99 MKK647 pKa = 10.45 KK648 pKa = 10.39 FADD651 pKa = 3.83 EE652 pKa = 4.02 ATQQVIDD659 pKa = 3.65 MSKK662 pKa = 11.27 GEE664 pKa = 4.13 FEE666 pKa = 4.34 KK667 pKa = 11.08 KK668 pKa = 9.59 PWAKK672 pKa = 9.59 WMSILEE678 pKa = 4.05 NAGFKK683 pKa = 9.79 GTTCLLDD690 pKa = 3.72 ANNYY694 pKa = 9.45 LIQPILGVSKK704 pKa = 9.24 LTARR708 pKa = 11.84 AWPRR712 pKa = 11.84 EE713 pKa = 4.07 LTPEE717 pKa = 3.53 VDD719 pKa = 3.84 LFDD722 pKa = 4.04 RR723 pKa = 11.84 LKK725 pKa = 10.72 AIHH728 pKa = 6.58 RR729 pKa = 11.84 HH730 pKa = 4.66 PCAYY734 pKa = 9.46 TISNLRR740 pKa = 11.84 ARR742 pKa = 11.84 AYY744 pKa = 10.6 ASDD747 pKa = 3.78 VKK749 pKa = 10.96 NDD751 pKa = 3.34 RR752 pKa = 11.84 TGSFTRR758 pKa = 11.84 SQDD761 pKa = 3.9 LAWKK765 pKa = 9.51 EE766 pKa = 4.14 AFTMICEE773 pKa = 4.41 SPRR776 pKa = 11.84 QRR778 pKa = 11.84 EE779 pKa = 3.85 VALTVIHH786 pKa = 6.45 GAGGSGKK793 pKa = 10.31 SFALQKK799 pKa = 10.79 YY800 pKa = 10.26 LEE802 pKa = 4.99 DD803 pKa = 4.58 CPDD806 pKa = 3.75 AEE808 pKa = 4.59 VKK810 pKa = 10.29 IVLPTNGLRR819 pKa = 11.84 MDD821 pKa = 4.57 WLAKK825 pKa = 10.16 LPKK828 pKa = 10.04 FPLTSVQTFEE838 pKa = 4.84 KK839 pKa = 10.77 ALXSQTAKK847 pKa = 10.54 VVIFDD852 pKa = 5.68 DD853 pKa = 4.12 YY854 pKa = 11.44 TKK856 pKa = 10.98 LPNGYY861 pKa = 8.72 IDD863 pKa = 5.19 AYY865 pKa = 10.75 LSLRR869 pKa = 11.84 TTVVWAILTGDD880 pKa = 3.39 SRR882 pKa = 11.84 QSSFHH887 pKa = 6.2 EE888 pKa = 4.25 MNEE891 pKa = 3.74 QALINGLTPAADD903 pKa = 3.41 HH904 pKa = 6.43 FSDD907 pKa = 3.84 YY908 pKa = 11.3 ARR910 pKa = 11.84 YY911 pKa = 9.33 YY912 pKa = 11.33 LNATHH917 pKa = 7.57 RR918 pKa = 11.84 NKK920 pKa = 10.15 QDD922 pKa = 3.49 LARR925 pKa = 11.84 MLGVYY930 pKa = 8.09 SEE932 pKa = 4.41 RR933 pKa = 11.84 TGLTTVSMSSRR944 pKa = 11.84 PLKK947 pKa = 9.21 EE948 pKa = 3.45 VHH950 pKa = 6.39 MLVPSLIQKK959 pKa = 7.87 KK960 pKa = 7.88 TYY962 pKa = 10.02 TEE964 pKa = 4.06 LGYY967 pKa = 8.7 KK968 pKa = 8.76 TSTYY972 pKa = 10.65 AGCQGITAPKK982 pKa = 9.78 VQVLLNSDD990 pKa = 3.51 TTLCTDD996 pKa = 3.14 KK997 pKa = 11.18 VLYY1000 pKa = 8.68 TALSRR1005 pKa = 11.84 AVHH1008 pKa = 6.57 SIHH1011 pKa = 7.45 FINTSASSAAFWDD1024 pKa = 3.89 KK1025 pKa = 11.56 LNATPYY1031 pKa = 10.8 LKK1033 pKa = 9.91 TFLSTVRR1040 pKa = 11.84 EE1041 pKa = 3.95 QSLASAKK1048 pKa = 9.88 PRR1050 pKa = 11.84 EE1051 pKa = 4.3 EE1052 pKa = 4.46 GPTPVAAPKK1061 pKa = 8.84 THH1063 pKa = 6.03 VPVEE1067 pKa = 3.98 NSNLCLEE1074 pKa = 4.52 GAVEE1078 pKa = 4.65 KK1079 pKa = 10.84 LLEE1082 pKa = 4.12 KK1083 pKa = 10.18 HH1084 pKa = 6.3 DD1085 pKa = 4.3 RR1086 pKa = 11.84 EE1087 pKa = 4.75 IFRR1090 pKa = 11.84 ADD1092 pKa = 3.47 HH1093 pKa = 5.6 GHH1095 pKa = 5.92 SNCVQTDD1102 pKa = 3.16 NPTIQLSSHH1111 pKa = 5.27 QQAKK1115 pKa = 10.41 DD1116 pKa = 3.39 QTLLWATIEE1125 pKa = 3.96 ARR1127 pKa = 11.84 LKK1129 pKa = 10.57 ISTPEE1134 pKa = 3.86 RR1135 pKa = 11.84 NLAEE1139 pKa = 4.0 YY1140 pKa = 10.8 LMKK1143 pKa = 10.59 RR1144 pKa = 11.84 EE1145 pKa = 4.1 VGDD1148 pKa = 4.1 VLWLHH1153 pKa = 6.41 YY1154 pKa = 10.09 KK1155 pKa = 10.41 NAMNLPEE1162 pKa = 4.24 EE1163 pKa = 5.04 PIPFEE1168 pKa = 3.6 QTLWDD1173 pKa = 3.6 TCAEE1177 pKa = 4.27 EE1178 pKa = 4.16 VQKK1181 pKa = 9.91 TYY1183 pKa = 10.87 LSKK1186 pKa = 10.58 PMHH1189 pKa = 5.62 MLKK1192 pKa = 10.46 NGEE1195 pKa = 4.27 LRR1197 pKa = 11.84 QSPDD1201 pKa = 2.83 FHH1203 pKa = 7.62 KK1204 pKa = 10.68 NTIALFLKK1212 pKa = 9.3 SQWVKK1217 pKa = 11.13 KK1218 pKa = 7.9 MEE1220 pKa = 4.25 KK1221 pKa = 10.35 LGADD1225 pKa = 3.86 KK1226 pKa = 10.45 IKK1228 pKa = 10.44 PGQTIASFQQAAVMLYY1244 pKa = 8.76 GTMARR1249 pKa = 11.84 YY1250 pKa = 7.15 MRR1252 pKa = 11.84 RR1253 pKa = 11.84 MRR1255 pKa = 11.84 EE1256 pKa = 4.07 VFQPTHH1262 pKa = 5.78 VFINCEE1268 pKa = 3.74 QSPEE1272 pKa = 4.21 GMSDD1276 pKa = 3.54 WIKK1279 pKa = 11.18 NEE1281 pKa = 3.8 WNFKK1285 pKa = 9.36 GNAYY1289 pKa = 10.52 ANDD1292 pKa = 3.62 FTQFDD1297 pKa = 3.87 QSQDD1301 pKa = 2.96 GAMLQFEE1308 pKa = 4.37 ILKK1311 pKa = 9.93 AKK1313 pKa = 9.62 HH1314 pKa = 5.9 HH1315 pKa = 6.67 SIPEE1319 pKa = 4.15 WVLEE1323 pKa = 4.41 GYY1325 pKa = 11.04 LDD1327 pKa = 3.45 IKK1329 pKa = 9.94 TNAHH1333 pKa = 5.84 VFTGTLGIMRR1343 pKa = 11.84 LTGEE1347 pKa = 4.77 GPTFDD1352 pKa = 6.01 ANTEE1356 pKa = 4.01 CNIAYY1361 pKa = 7.09 THH1363 pKa = 6.8 LRR1365 pKa = 11.84 FNVPKK1370 pKa = 9.79 GTAQAYY1376 pKa = 10.26 AGDD1379 pKa = 4.44 DD1380 pKa = 3.65 SVLACIPEE1388 pKa = 3.98 EE1389 pKa = 4.06 RR1390 pKa = 11.84 EE1391 pKa = 4.12 SFKK1394 pKa = 9.99 MWKK1397 pKa = 10.0 NKK1399 pKa = 10.47 LEE1401 pKa = 4.09 LTAKK1405 pKa = 10.35 PEE1407 pKa = 4.18 YY1408 pKa = 9.25 FPQMQGEE1415 pKa = 4.32 WAQFCGFLFTPLGALKK1431 pKa = 10.5 EE1432 pKa = 4.12 PLKK1435 pKa = 10.24 MLAVVEE1441 pKa = 4.12 LAKK1444 pKa = 10.58 QRR1446 pKa = 11.84 GEE1448 pKa = 3.86 LKK1450 pKa = 10.82 KK1451 pKa = 10.79 NMRR1454 pKa = 11.84 SYY1456 pKa = 11.68 ALDD1459 pKa = 3.21 VLLNYY1464 pKa = 9.65 QHH1466 pKa = 7.07 GDD1468 pKa = 3.14 ALQHH1472 pKa = 5.68 VFSEE1476 pKa = 4.61 KK1477 pKa = 10.03 QLEE1480 pKa = 4.05 AHH1482 pKa = 6.41 QITVRR1487 pKa = 11.84 NLIKK1491 pKa = 10.74 FGGSSILEE1499 pKa = 4.1 NCC1501 pKa = 4.35
Molecular weight: 169.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.82
IPC2_protein 5.741
IPC_protein 5.804
Toseland 6.211
ProMoST 6.148
Dawson 6.059
Bjellqvist 6.071
Wikipedia 6.059
Rodwell 6.046
Grimsley 6.364
Solomon 6.059
Lehninger 6.046
Nozaki 6.326
DTASelect 6.504
Thurlkill 6.517
EMBOSS 6.478
Sillero 6.427
Patrickios 4.317
IPC_peptide 6.071
IPC2_peptide 6.389
IPC2.peptide.svr19 6.393
Protein with the highest isoelectric point:
>tr|G3LHV7|G3LHV7_9VIRU Movement protein TGBp3 OS=Tamus red mosaic virus OX=1081702 PE=3 SV=1
MM1 pKa = 7.78 SGRR4 pKa = 11.84 QVHH7 pKa = 5.99 LTPPVDD13 pKa = 3.45 HH14 pKa = 7.02 SNSVLALVVGVSIALVVFSITRR36 pKa = 11.84 NTSPHH41 pKa = 6.35 VGDD44 pKa = 5.1 NIHH47 pKa = 6.37 SLPHH51 pKa = 5.77 GGCYY55 pKa = 9.65 KK56 pKa = 10.88 DD57 pKa = 3.65 GTKK60 pKa = 10.19 SIYY63 pKa = 9.53 YY64 pKa = 8.28 GKK66 pKa = 9.34 PGNSVRR72 pKa = 11.84 QPARR76 pKa = 11.84 GAALVLVIFLPILIYY91 pKa = 10.44 AIHH94 pKa = 6.61 KK95 pKa = 9.64 FEE97 pKa = 4.16 SRR99 pKa = 11.84 RR100 pKa = 11.84 TRR102 pKa = 11.84 VSACGHH108 pKa = 6.15 IACPSGGATQHH119 pKa = 6.06
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.225
IPC2_protein 9.355
IPC_protein 9.56
Toseland 9.765
ProMoST 9.633
Dawson 10.058
Bjellqvist 9.853
Wikipedia 10.277
Rodwell 10.262
Grimsley 10.16
Solomon 10.131
Lehninger 10.087
Nozaki 9.94
DTASelect 9.809
Thurlkill 9.897
EMBOSS 10.204
Sillero 10.014
Patrickios 7.512
IPC_peptide 10.116
IPC2_peptide 9.033
IPC2.peptide.svr19 8.107
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2135
64
1501
427.0
47.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.244 ± 0.383
1.358 ± 0.241
4.496 ± 0.549
7.026 ± 1.141
4.871 ± 0.469
5.386 ± 1.108
3.232 ± 0.837
4.356 ± 0.595
6.511 ± 0.787
9.836 ± 0.754
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.537
4.309 ± 0.228
5.574 ± 0.957
4.169 ± 0.887
4.309 ± 0.348
7.119 ± 1.055
6.792 ± 0.658
6.37 ± 1.371
1.311 ± 0.49
2.436 ± 0.304
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here