Chlorogloea sp. CCALA 695
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4747 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T1E8H0|A0A2T1E8H0_9CHRO Phosphate starvation-inducible protein PhoH OS=Chlorogloea sp. CCALA 695 OX=2107693 GN=C7B70_19500 PE=4 SV=1
MM1 pKa = 7.71 DD2 pKa = 3.75 TPFTFEE8 pKa = 5.23 QEE10 pKa = 3.67 TDD12 pKa = 3.1 LNAWYY17 pKa = 9.27 DD18 pKa = 3.78 QLNSAWQAKK27 pKa = 9.17 IADD30 pKa = 4.2 PNSPEE35 pKa = 4.15 SLSLAEE41 pKa = 5.51 AEE43 pKa = 4.85 AYY45 pKa = 9.07 WEE47 pKa = 4.11 AEE49 pKa = 3.42 RR50 pKa = 11.84 EE51 pKa = 4.24 YY52 pKa = 10.82 YY53 pKa = 10.03 HH54 pKa = 7.72 DD55 pKa = 3.93 RR56 pKa = 11.84 FLYY59 pKa = 10.79 ADD61 pKa = 3.85 IFF63 pKa = 4.01
Molecular weight: 7.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.884
IPC_protein 3.77
Toseland 3.592
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.503
Solomon 3.719
Lehninger 3.668
Nozaki 3.884
DTASelect 4.012
Thurlkill 3.643
EMBOSS 3.681
Sillero 3.884
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A2T1ENE8|A0A2T1ENE8_9CHRO DUF3893 domain-containing protein OS=Chlorogloea sp. CCALA 695 OX=2107693 GN=C7B70_04265 PE=4 SV=1
MM1 pKa = 7.58 TEE3 pKa = 4.59 FIVPQTSAKK12 pKa = 8.91 AQKK15 pKa = 10.26 HH16 pKa = 5.34 SAPQDD21 pKa = 3.51 RR22 pKa = 11.84 IRR24 pKa = 11.84 RR25 pKa = 11.84 WVWKK29 pKa = 10.12 LCIATLILMAIGSTTRR45 pKa = 11.84 VMNAGLACPDD55 pKa = 3.16 WPLCYY60 pKa = 9.36 GTLVPRR66 pKa = 11.84 AQMNLQVFLEE76 pKa = 4.36 WFHH79 pKa = 7.71 RR80 pKa = 11.84 LDD82 pKa = 3.41 AALIGVGALSLSVICWWNRR101 pKa = 11.84 RR102 pKa = 11.84 QLPGWLPWAATFALGLIMFQGVLGGLTVTQLLRR135 pKa = 11.84 FDD137 pKa = 4.01 IVTAHH142 pKa = 7.45 LGTALLFFSTLLVMGTALTPYY163 pKa = 9.89 QGTGTAGKK171 pKa = 10.06 LPWLGLTAAILVYY184 pKa = 10.38 LQSLLGALVASRR196 pKa = 11.84 WALHH200 pKa = 5.34 QCLTVKK206 pKa = 10.46 ALCSVMYY213 pKa = 9.19 SHH215 pKa = 7.43 IGFVVLPSLAIIAVVWMSWRR235 pKa = 11.84 TPALHH240 pKa = 5.92 PTLRR244 pKa = 11.84 LLANMTGGLLLLQILLGIATLRR266 pKa = 11.84 LHH268 pKa = 6.52 LQIEE272 pKa = 4.74 PLTVAHH278 pKa = 5.76 QAVGAALLGSLVIFTVLGWRR298 pKa = 11.84 DD299 pKa = 3.41 SASAPITGLRR309 pKa = 11.84 AFLAARR315 pKa = 4.08
Molecular weight: 34.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.545
IPC_protein 10.306
Toseland 10.365
ProMoST 10.335
Dawson 10.526
Bjellqvist 10.292
Wikipedia 10.745
Rodwell 10.687
Grimsley 10.599
Solomon 10.613
Lehninger 10.584
Nozaki 10.452
DTASelect 10.262
Thurlkill 10.409
EMBOSS 10.774
Sillero 10.482
Patrickios 10.394
IPC_peptide 10.613
IPC2_peptide 9.692
IPC2.peptide.svr19 8.526
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4747
0
4747
1414214
22
2885
297.9
33.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.447 ± 0.038
1.028 ± 0.012
4.8 ± 0.025
6.083 ± 0.033
3.81 ± 0.019
6.504 ± 0.037
1.723 ± 0.017
6.735 ± 0.025
5.079 ± 0.031
11.06 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.806 ± 0.016
4.341 ± 0.027
4.62 ± 0.027
5.376 ± 0.035
5.07 ± 0.025
6.596 ± 0.027
5.76 ± 0.028
6.761 ± 0.032
1.395 ± 0.016
3.007 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here