Helminthosporium victoriae 145S virus
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8JVB5|Q8JVB5_9VIRU Hv145SV-protein 3 OS=Helminthosporium victoriae 145S virus OX=164750 PE=2 SV=1
MM1 pKa = 7.32 EE2 pKa = 6.42 AIGHH6 pKa = 6.03 LLKK9 pKa = 10.94 GVAKK13 pKa = 10.48 VNGDD17 pKa = 3.31 RR18 pKa = 11.84 RR19 pKa = 11.84 VDD21 pKa = 3.37 STMGSMVEE29 pKa = 3.83 PTVNSRR35 pKa = 11.84 GGMRR39 pKa = 11.84 IEE41 pKa = 4.3 RR42 pKa = 11.84 NSKK45 pKa = 8.96 VRR47 pKa = 11.84 EE48 pKa = 4.03 VNLARR53 pKa = 11.84 AFRR56 pKa = 11.84 KK57 pKa = 10.0 SRR59 pKa = 11.84 ITWKK63 pKa = 10.74 LPAEE67 pKa = 4.21 YY68 pKa = 9.54 RR69 pKa = 11.84 DD70 pKa = 3.86 EE71 pKa = 4.07 EE72 pKa = 4.99 SMNIDD77 pKa = 4.39 LRR79 pKa = 11.84 PNLYY83 pKa = 9.28 TVVMPAGCGKK93 pKa = 7.13 TTIANEE99 pKa = 4.06 FNCIDD104 pKa = 3.65 VDD106 pKa = 4.03 DD107 pKa = 4.74 LAGVDD112 pKa = 4.23 SRR114 pKa = 11.84 AEE116 pKa = 3.6 LMGWLKK122 pKa = 10.6 EE123 pKa = 4.15 ISDD126 pKa = 3.89 GTASEE131 pKa = 3.95 KK132 pKa = 10.97 SEE134 pKa = 3.65 WVRR137 pKa = 11.84 IVNKK141 pKa = 10.45 ALDD144 pKa = 3.45 KK145 pKa = 10.66 MVFEE149 pKa = 5.13 EE150 pKa = 4.36 PVVMLVHH157 pKa = 6.84 DD158 pKa = 4.7 HH159 pKa = 6.19 LTAKK163 pKa = 10.56 LVGSIRR169 pKa = 11.84 AGTIMTPKK177 pKa = 10.42 DD178 pKa = 3.34 QVKK181 pKa = 10.37 GMNKK185 pKa = 9.26 NRR187 pKa = 11.84 DD188 pKa = 3.82 KK189 pKa = 10.88 KK190 pKa = 9.69 WNDD193 pKa = 2.53 IFEE196 pKa = 4.65 VTWAMAHH203 pKa = 6.74 DD204 pKa = 4.55 SNVKK208 pKa = 10.0 RR209 pKa = 11.84 KK210 pKa = 8.9 WYY212 pKa = 9.02 TPTTAEE218 pKa = 4.4 CYY220 pKa = 9.63 RR221 pKa = 11.84 RR222 pKa = 11.84 LARR225 pKa = 11.84 VMANLHH231 pKa = 6.43 CEE233 pKa = 4.06 VPPPNVCFFDD243 pKa = 4.4 AGMYY247 pKa = 10.82 DD248 pKa = 3.34 MEE250 pKa = 4.58 LFKK253 pKa = 11.41 GNEE256 pKa = 3.67 NRR258 pKa = 11.84 IEE260 pKa = 4.0 EE261 pKa = 4.18 LVEE264 pKa = 3.92 LNEE267 pKa = 5.12 AGLCPSLAIHH277 pKa = 6.62 KK278 pKa = 9.64 CCIKK282 pKa = 10.78 NGMRR286 pKa = 11.84 TEE288 pKa = 4.3 MPWVSYY294 pKa = 11.06 SKK296 pKa = 10.81 LAGRR300 pKa = 11.84 LANRR304 pKa = 11.84 KK305 pKa = 8.7 AAGVAGKK312 pKa = 10.49 KK313 pKa = 10.05 SGAIKK318 pKa = 10.52 KK319 pKa = 10.23 SLLWDD324 pKa = 3.41 KK325 pKa = 11.11 FNLGEE330 pKa = 4.7 HH331 pKa = 6.66 EE332 pKa = 4.55 DD333 pKa = 3.6 AVAIDD338 pKa = 3.62 KK339 pKa = 11.05 LLVKK343 pKa = 10.8 SSDD346 pKa = 3.18 AFSNCVVLWWKK357 pKa = 8.49 TVMQEE362 pKa = 3.6 YY363 pKa = 10.53 DD364 pKa = 3.39 IANHH368 pKa = 6.06 IYY370 pKa = 11.07 KK371 pKa = 9.73 MILGVQEE378 pKa = 4.25 KK379 pKa = 8.59 EE380 pKa = 3.89 WRR382 pKa = 11.84 DD383 pKa = 3.41 VLTDD387 pKa = 3.49 IGQVLLTGAIGNVYY401 pKa = 10.14 VSPEE405 pKa = 3.88 SANVVMSARR414 pKa = 11.84 AFSDD418 pKa = 3.62 SEE420 pKa = 4.53 GVTKK424 pKa = 10.71 ACDD427 pKa = 3.21 NWSLEE432 pKa = 4.12 MNFEE436 pKa = 4.18 EE437 pKa = 4.62 TKK439 pKa = 10.51 SDD441 pKa = 3.49 VMVMIDD447 pKa = 4.31 RR448 pKa = 11.84 RR449 pKa = 11.84 VLDD452 pKa = 4.18 KK453 pKa = 11.16 VSCGDD458 pKa = 3.55 GEE460 pKa = 4.67 YY461 pKa = 9.83 MCAEE465 pKa = 4.44 AEE467 pKa = 3.98 WCRR470 pKa = 11.84 WKK472 pKa = 10.96 LDD474 pKa = 3.28 DD475 pKa = 4.94 CGLVRR480 pKa = 11.84 GVVNITNMEE489 pKa = 4.05 ADD491 pKa = 3.24 DD492 pKa = 3.63 QYY494 pKa = 11.76 RR495 pKa = 11.84 VVRR498 pKa = 11.84 CIKK501 pKa = 10.24 AIEE504 pKa = 4.5 GGIQLEE510 pKa = 4.23 PVIGSDD516 pKa = 3.47 QYY518 pKa = 11.15 LAEE521 pKa = 4.08 MRR523 pKa = 11.84 NARR526 pKa = 11.84 EE527 pKa = 3.68 VEE529 pKa = 4.2 RR530 pKa = 11.84 IVRR533 pKa = 11.84 MSAYY537 pKa = 9.11 KK538 pKa = 10.0 VSAMGTILTTDD549 pKa = 4.25 FEE551 pKa = 4.63 ALPRR555 pKa = 11.84 TRR557 pKa = 11.84 CALEE561 pKa = 5.62 AMFEE565 pKa = 4.13 WTKK568 pKa = 10.21 WLSGNYY574 pKa = 9.03 EE575 pKa = 4.06 EE576 pKa = 5.01 YY577 pKa = 10.53 LYY579 pKa = 11.16 DD580 pKa = 3.42 SVRR583 pKa = 11.84 PTRR586 pKa = 11.84 VIKK589 pKa = 8.98 EE590 pKa = 3.56 ICIPSSKK597 pKa = 10.91 LEE599 pKa = 4.17 MYY601 pKa = 10.72 SGLTTMFMMGDD612 pKa = 3.22 KK613 pKa = 10.09 EE614 pKa = 4.23 AAVVLAANAMRR625 pKa = 11.84 NRR627 pKa = 11.84 RR628 pKa = 11.84 EE629 pKa = 3.62 WTQDD633 pKa = 2.62 VEE635 pKa = 4.36 AYY637 pKa = 10.25 HH638 pKa = 5.58 MCEE641 pKa = 3.87 VRR643 pKa = 11.84 STEE646 pKa = 4.08 SMRR649 pKa = 11.84 LISKK653 pKa = 8.18 SFRR656 pKa = 11.84 EE657 pKa = 4.58 AKK659 pKa = 10.15 GHH661 pKa = 4.68 IHH663 pKa = 7.33 RR664 pKa = 11.84 ICSSRR669 pKa = 11.84 KK670 pKa = 9.39 KK671 pKa = 9.75 KK672 pKa = 10.78 DD673 pKa = 3.21 SVVWVEE679 pKa = 4.11 DD680 pKa = 3.2 STMEE684 pKa = 4.98 DD685 pKa = 3.73 MIDD688 pKa = 3.9 EE689 pKa = 4.26 ITVSIRR695 pKa = 11.84 EE696 pKa = 4.07 TMLCGDD702 pKa = 4.33 FDD704 pKa = 4.03 FTEE707 pKa = 4.44 VSSASGG713 pKa = 3.09
Molecular weight: 80.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.534
IPC2_protein 5.614
IPC_protein 5.601
Toseland 5.728
ProMoST 5.842
Dawson 5.728
Bjellqvist 5.792
Wikipedia 5.664
Rodwell 5.677
Grimsley 5.741
Solomon 5.728
Lehninger 5.703
Nozaki 5.931
DTASelect 6.084
Thurlkill 5.97
EMBOSS 5.919
Sillero 6.02
Patrickios 4.291
IPC_peptide 5.741
IPC2_peptide 5.995
IPC2.peptide.svr19 6.11
Protein with the highest isoelectric point:
>tr|Q8JVB7|Q8JVB7_9VIRU RNA-directed RNA polymerase OS=Helminthosporium victoriae 145S virus OX=164750 PE=2 SV=1
MM1 pKa = 7.82 ADD3 pKa = 3.33 MFNAGKK9 pKa = 10.23 YY10 pKa = 9.54 DD11 pKa = 3.58 RR12 pKa = 11.84 RR13 pKa = 11.84 QAIYY17 pKa = 8.28 MAVRR21 pKa = 11.84 AGAPAFKK28 pKa = 10.37 KK29 pKa = 10.54 AVEE32 pKa = 4.31 TVQKK36 pKa = 10.64 LEE38 pKa = 3.94 HH39 pKa = 6.9 WDD41 pKa = 3.49 PTKK44 pKa = 10.92 LPNKK48 pKa = 9.0 MADD51 pKa = 3.61 GKK53 pKa = 10.18 PMLFKK58 pKa = 10.82 GLQAVEE64 pKa = 4.82 SYY66 pKa = 9.26 MEE68 pKa = 4.61 RR69 pKa = 11.84 YY70 pKa = 9.06 SASSLDD76 pKa = 3.64 ALSEE80 pKa = 4.21 EE81 pKa = 4.81 KK82 pKa = 10.91 NSFGYY87 pKa = 10.94 GIFGDD92 pKa = 3.47 IRR94 pKa = 11.84 RR95 pKa = 11.84 PAMLGANTVGIDD107 pKa = 3.44 VTVEE111 pKa = 3.54 WGSTEE116 pKa = 3.84 VDD118 pKa = 2.93 AEE120 pKa = 4.14 MHH122 pKa = 5.98 NGEE125 pKa = 4.27 NRR127 pKa = 11.84 KK128 pKa = 9.16 MIVSTGTTTVRR139 pKa = 11.84 NEE141 pKa = 3.84 HH142 pKa = 5.92 GKK144 pKa = 10.21 DD145 pKa = 2.8 RR146 pKa = 11.84 AGISKK151 pKa = 8.7 ATGWDD156 pKa = 3.07 RR157 pKa = 11.84 TEE159 pKa = 4.66 CYY161 pKa = 10.77 SMSPSDD167 pKa = 3.93 VQTLCTLIDD176 pKa = 3.3 TGRR179 pKa = 11.84 AGFNKK184 pKa = 8.51 YY185 pKa = 7.54 TRR187 pKa = 11.84 LVKK190 pKa = 10.66 GMLVYY195 pKa = 10.83 LDD197 pKa = 4.57 LLNNGKK203 pKa = 8.46 QAIKK207 pKa = 10.52 KK208 pKa = 9.09 RR209 pKa = 11.84 IPNMISYY216 pKa = 10.0 DD217 pKa = 3.75 LKK219 pKa = 11.18 SVLGSYY225 pKa = 7.02 QHH227 pKa = 7.18 RR228 pKa = 11.84 DD229 pKa = 2.57 RR230 pKa = 11.84 SYY232 pKa = 10.49 IYY234 pKa = 10.26 CSNPDD239 pKa = 3.31 SPEE242 pKa = 3.68 YY243 pKa = 10.29 RR244 pKa = 11.84 VVLSLMSEE252 pKa = 4.91 AYY254 pKa = 9.8 PNDD257 pKa = 3.61 DD258 pKa = 3.37 MTCYY262 pKa = 10.4 GVANIPADD270 pKa = 3.55 GEE272 pKa = 4.67 TNVIVVNGSAGSSVNYY288 pKa = 9.8 HH289 pKa = 6.11 VEE291 pKa = 4.0 LTPQMVMASITQYY304 pKa = 9.91 ATEE307 pKa = 4.34 SGIADD312 pKa = 3.95 EE313 pKa = 5.36 LEE315 pKa = 4.23 SALVCASSLYY325 pKa = 9.63 HH326 pKa = 5.87 NRR328 pKa = 11.84 YY329 pKa = 7.95 LARR332 pKa = 11.84 VGLPRR337 pKa = 11.84 VVSSIDD343 pKa = 3.75 LIMPMFRR350 pKa = 11.84 PANEE354 pKa = 3.89 VRR356 pKa = 11.84 CARR359 pKa = 11.84 PSVAKK364 pKa = 10.04 EE365 pKa = 3.42 LAISIGKK372 pKa = 8.18 LHH374 pKa = 5.65 QMCMFLTIKK383 pKa = 10.51 DD384 pKa = 3.47 ILVAARR390 pKa = 11.84 GSTKK394 pKa = 10.72 AGFNYY399 pKa = 10.1 SSVVEE404 pKa = 4.65 SYY406 pKa = 9.49 LTTQEE411 pKa = 4.14 EE412 pKa = 5.27 VVSVMNASVTPLRR425 pKa = 11.84 LLEE428 pKa = 4.36 MTPQMKK434 pKa = 9.5 WMYY437 pKa = 10.39 KK438 pKa = 9.28 IDD440 pKa = 3.94 AEE442 pKa = 4.55 AMQDD446 pKa = 3.69 LDD448 pKa = 3.71 SLSIFEE454 pKa = 5.11 VFWLCDD460 pKa = 3.27 GGVASVRR467 pKa = 11.84 NGGVMAFKK475 pKa = 10.63 KK476 pKa = 10.46 GVSDD480 pKa = 3.62 MMTDD484 pKa = 3.39 NPYY487 pKa = 10.77 HH488 pKa = 7.17 DD489 pKa = 4.22 ILRR492 pKa = 11.84 KK493 pKa = 9.49 EE494 pKa = 3.97 LAKK497 pKa = 11.04 SNVVFDD503 pKa = 4.52 FSKK506 pKa = 10.69 LPKK509 pKa = 10.63 GNFTIASRR517 pKa = 11.84 YY518 pKa = 9.03 IRR520 pKa = 11.84 DD521 pKa = 3.6 ANEE524 pKa = 3.62 VVLPKK529 pKa = 10.51 LEE531 pKa = 4.28 YY532 pKa = 8.12 VTEE535 pKa = 4.06 RR536 pKa = 11.84 VLIARR541 pKa = 11.84 EE542 pKa = 3.91 CDD544 pKa = 3.74 YY545 pKa = 11.3 NPHH548 pKa = 6.84 DD549 pKa = 4.09 RR550 pKa = 11.84 VEE552 pKa = 4.49 HH553 pKa = 6.44 IINNRR558 pKa = 11.84 TRR560 pKa = 11.84 VTLSARR566 pKa = 11.84 KK567 pKa = 8.57 EE568 pKa = 3.93 AMEE571 pKa = 4.21 SGRR574 pKa = 11.84 TKK576 pKa = 10.65 VAWGDD581 pKa = 3.58 IEE583 pKa = 4.51 VEE585 pKa = 4.13 RR586 pKa = 11.84 PTGKK590 pKa = 10.69 GSDD593 pKa = 3.5 EE594 pKa = 4.4 SEE596 pKa = 4.06 SVLRR600 pKa = 11.84 FGSSDD605 pKa = 2.91 GRR607 pKa = 11.84 QSPGIEE613 pKa = 3.8 MFGLKK618 pKa = 9.97 SEE620 pKa = 3.98 ARR622 pKa = 11.84 EE623 pKa = 3.77 RR624 pKa = 11.84 LEE626 pKa = 3.75 KK627 pKa = 10.35 RR628 pKa = 11.84 RR629 pKa = 11.84 SLVSPPPFRR638 pKa = 11.84 QLSEE642 pKa = 4.13 SASTRR647 pKa = 11.84 RR648 pKa = 11.84 AEE650 pKa = 3.97 RR651 pKa = 11.84 LSVSSHH657 pKa = 4.77 GSRR660 pKa = 11.84 RR661 pKa = 11.84 SISVDD666 pKa = 3.28 LEE668 pKa = 4.24 SVRR671 pKa = 11.84 SHH673 pKa = 6.78 SVDD676 pKa = 3.18 GDD678 pKa = 3.74 DD679 pKa = 6.07 DD680 pKa = 3.87 KK681 pKa = 11.81 TPTQSQNLRR690 pKa = 11.84 KK691 pKa = 9.91 RR692 pKa = 11.84 FDD694 pKa = 3.59 FSVLQKK700 pKa = 11.04 AVDD703 pKa = 3.84 EE704 pKa = 4.55 KK705 pKa = 11.42 KK706 pKa = 9.88 MPGSYY711 pKa = 10.46 EE712 pKa = 3.95 STPEE716 pKa = 3.74 KK717 pKa = 10.3 TEE719 pKa = 3.69 PTVTVEE725 pKa = 4.69 KK726 pKa = 10.64 IPGVKK731 pKa = 9.67 SSMGVSEE738 pKa = 4.42 EE739 pKa = 4.11 VEE741 pKa = 3.72 NDD743 pKa = 2.43 KK744 pKa = 11.08 RR745 pKa = 11.84 AIYY748 pKa = 9.92 KK749 pKa = 10.3 ADD751 pKa = 3.72 IIGSDD756 pKa = 4.14 RR757 pKa = 11.84 INGISAVNFHH767 pKa = 6.54 KK768 pKa = 10.73 LFRR771 pKa = 11.84 EE772 pKa = 4.03 RR773 pKa = 11.84 FDD775 pKa = 3.75 EE776 pKa = 4.39 KK777 pKa = 10.74 QVSPSVMARR786 pKa = 11.84 LMAVLKK792 pKa = 10.76 NVGVRR797 pKa = 11.84 VNIAAMTADD806 pKa = 5.05 EE807 pKa = 4.85 INALMKK813 pKa = 10.24 MRR815 pKa = 11.84 DD816 pKa = 3.74 GYY818 pKa = 11.1 DD819 pKa = 2.77 RR820 pKa = 11.84 SYY822 pKa = 11.0 RR823 pKa = 11.84 SSEE826 pKa = 3.89 SGHH829 pKa = 5.04 NRR831 pKa = 11.84 EE832 pKa = 3.98 FRR834 pKa = 11.84 EE835 pKa = 3.57 PDD837 pKa = 3.13 GRR839 pKa = 11.84 VLTMEE844 pKa = 3.87 INLRR848 pKa = 11.84 RR849 pKa = 11.84 MRR851 pKa = 11.84 CDD853 pKa = 3.3 RR854 pKa = 11.84 DD855 pKa = 3.48 KK856 pKa = 11.54 KK857 pKa = 10.09 PLRR860 pKa = 11.84 EE861 pKa = 4.02 ADD863 pKa = 3.67 EE864 pKa = 4.54 TGLIPDD870 pKa = 3.78 HH871 pKa = 6.2 MARR874 pKa = 11.84 KK875 pKa = 9.77 LGRR878 pKa = 11.84 EE879 pKa = 4.0 FFMAHH884 pKa = 6.66 HH885 pKa = 6.47 EE886 pKa = 4.01 RR887 pKa = 11.84 DD888 pKa = 3.55 MILARR893 pKa = 11.84 VVV895 pKa = 2.92
Molecular weight: 100.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.942
IPC2_protein 7.41
IPC_protein 7.293
Toseland 7.117
ProMoST 7.849
Dawson 8.009
Bjellqvist 8.273
Wikipedia 7.936
Rodwell 8.024
Grimsley 7.205
Solomon 8.068
Lehninger 8.083
Nozaki 8.434
DTASelect 8.097
Thurlkill 8.156
EMBOSS 8.229
Sillero 8.434
Patrickios 4.584
IPC_peptide 8.053
IPC2_peptide 7.249
IPC2.peptide.svr19 7.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3533
713
1086
883.3
99.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.935 ± 0.319
1.755 ± 0.26
6.595 ± 0.138
7.614 ± 0.422
3.085 ± 0.382
6.453 ± 0.201
2.123 ± 0.193
4.812 ± 0.129
6.482 ± 0.224
7.444 ± 0.372
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.095 ± 0.218
4.217 ± 0.214
3.029 ± 0.275
1.811 ± 0.217
6.736 ± 0.376
7.416 ± 0.944
5.067 ± 0.39
8.491 ± 0.128
1.642 ± 0.316
3.198 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here