Balneolaceae bacterium YR4-1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; Balneolia; Balneolales; Balneolaceae; unclassified Balneolaceae

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1SWV8|A0A6M1SWV8_9BACT Cation transporter OS=Balneolaceae bacterium YR4-1 OX=2709311 GN=G3570_02790 PE=4 SV=1
MM1 pKa = 6.98MRR3 pKa = 11.84YY4 pKa = 9.9LGILLSVFIFTSCIEE19 pKa = 4.08SSGTIGPQGPEE30 pKa = 4.39GPPGPIGQAFEE41 pKa = 4.35VEE43 pKa = 4.44ADD45 pKa = 3.57FTEE48 pKa = 4.48NNGYY52 pKa = 6.79TQIFEE57 pKa = 4.33IPPSVEE63 pKa = 4.06IYY65 pKa = 10.7SSDD68 pKa = 2.86IVAVYY73 pKa = 9.43LLWEE77 pKa = 4.29VDD79 pKa = 3.32EE80 pKa = 4.55STGNDD85 pKa = 2.68VWQQLPVSVFFNDD98 pKa = 3.74GQLQYY103 pKa = 11.79AFDD106 pKa = 3.75HH107 pKa = 6.01TVADD111 pKa = 3.61VKK113 pKa = 11.19LFLTGDD119 pKa = 3.71TDD121 pKa = 4.55LSTVGDD127 pKa = 5.14GYY129 pKa = 9.51TLDD132 pKa = 3.35QIFRR136 pKa = 11.84VVVLPAEE143 pKa = 4.39YY144 pKa = 10.08VQEE147 pKa = 4.43NGVDD151 pKa = 3.59MSNMEE156 pKa = 4.3EE157 pKa = 3.95VMGAVKK163 pKa = 10.48KK164 pKa = 10.98GDD166 pKa = 3.34IQRR169 pKa = 11.84ITLL172 pKa = 3.66

Molecular weight: 18.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1ST56|A0A6M1ST56_9BACT Na-K-Cl cotransporter OS=Balneolaceae bacterium YR4-1 OX=2709311 GN=G3570_01735 PE=4 SV=1
MM1 pKa = 7.64IKK3 pKa = 10.45NPSHH7 pKa = 6.71PKK9 pKa = 9.37KK10 pKa = 9.81GHH12 pKa = 4.87IRR14 pKa = 11.84TRR16 pKa = 11.84EE17 pKa = 4.0CKK19 pKa = 8.44FTKK22 pKa = 10.45AGIEE26 pKa = 4.11YY27 pKa = 9.39IDD29 pKa = 3.91YY30 pKa = 10.87KK31 pKa = 10.53DD32 pKa = 3.61TEE34 pKa = 4.18VLQRR38 pKa = 11.84FMNDD42 pKa = 2.58QGRR45 pKa = 11.84ILPRR49 pKa = 11.84RR50 pKa = 11.84VTGTSAKK57 pKa = 9.31YY58 pKa = 9.38QRR60 pKa = 11.84QLSRR64 pKa = 11.84AIKK67 pKa = 9.16RR68 pKa = 11.84ARR70 pKa = 11.84FLGMIPYY77 pKa = 9.99VADD80 pKa = 3.47NLRR83 pKa = 3.9

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3201

0

3201

1117832

35

2881

349.2

39.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.731 ± 0.04

0.634 ± 0.01

6.004 ± 0.033

7.736 ± 0.053

4.656 ± 0.032

7.068 ± 0.042

1.91 ± 0.018

7.069 ± 0.037

5.761 ± 0.045

9.477 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.021

4.884 ± 0.036

3.882 ± 0.022

3.63 ± 0.029

4.695 ± 0.032

6.744 ± 0.038

5.379 ± 0.031

6.311 ± 0.028

1.242 ± 0.019

3.754 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski