Ornithorhynchus anatinus (Duckbill platypus)
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 32824 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F7FBF3|F7FBF3_ORNAN Nuclear factor related to kappaB binding protein OS=Ornithorhynchus anatinus OX=9258 GN=NFRKB PE=4 SV=2
MM1 pKa = 7.45 LSSSAILDD9 pKa = 4.05 HH10 pKa = 7.09 PPSPMDD16 pKa = 3.15 TSEE19 pKa = 4.26 LHH21 pKa = 6.18 FAPEE25 pKa = 4.11 PGAGLGLDD33 pKa = 4.42 LAASHH38 pKa = 7.11 LDD40 pKa = 3.39 GMDD43 pKa = 2.95 WLEE46 pKa = 4.35 LSGGPAASLAPLGAAAPSLFSTDD69 pKa = 3.84 FLDD72 pKa = 4.44 GHH74 pKa = 6.85 DD75 pKa = 5.24 LQLHH79 pKa = 6.18 WDD81 pKa = 3.6 SCLL84 pKa = 3.37
Molecular weight: 8.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.91
IPC_protein 3.846
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.681
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.999
DTASelect 4.253
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A6I8ND41|A0A6I8ND41_ORNAN Non-specific serine/threonine protein kinase OS=Ornithorhynchus anatinus OX=9258 PE=3 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.12 WRR6 pKa = 11.84 KK7 pKa = 9.1 KK8 pKa = 9.32 RR9 pKa = 11.84 MRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 10.08 RR15 pKa = 11.84 KK16 pKa = 8.21 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.46 MRR21 pKa = 11.84 QRR23 pKa = 11.84 SKK25 pKa = 11.41
Molecular weight: 3.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17390
15434
32824
22389980
22
8244
682.1
75.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.241 ± 0.014
2.103 ± 0.011
4.893 ± 0.009
7.057 ± 0.018
3.621 ± 0.009
7.075 ± 0.019
2.442 ± 0.006
4.214 ± 0.012
5.506 ± 0.015
9.871 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.995 ± 0.005
3.497 ± 0.009
6.737 ± 0.022
4.542 ± 0.011
6.171 ± 0.015
8.134 ± 0.017
5.111 ± 0.01
6.037 ± 0.01
1.209 ± 0.004
2.543 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here