Firmicutes bacterium CAG:170
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2216 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6MMU7|R6MMU7_9FIRM Conserved domain protein OS=Firmicutes bacterium CAG:170 OX=1263006 GN=BN515_01290 PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 NRR4 pKa = 11.84 AGVILTALLLALLCGCAPARR24 pKa = 11.84 PAAVYY29 pKa = 9.21 QNPIDD34 pKa = 5.39 FYY36 pKa = 11.37 YY37 pKa = 10.98 CDD39 pKa = 4.9 LEE41 pKa = 4.78 DD42 pKa = 3.61 PHH44 pKa = 8.54 GGALGALAKK53 pKa = 9.29 QTVDD57 pKa = 3.99 LVDD60 pKa = 3.71 PEE62 pKa = 4.27 ITVEE66 pKa = 4.31 EE67 pKa = 4.24 ILTRR71 pKa = 11.84 YY72 pKa = 9.19 LAGPDD77 pKa = 4.05 DD78 pKa = 4.54 PALAAPFPDD87 pKa = 3.84 GLTCEE92 pKa = 4.55 SVTILDD98 pKa = 3.57 GVLCVRR104 pKa = 11.84 FGEE107 pKa = 4.88 AYY109 pKa = 10.39 AEE111 pKa = 3.96 LSGIRR116 pKa = 11.84 LSLTNACLTKK126 pKa = 10.25 TLLQIPFVEE135 pKa = 4.69 SVQIEE140 pKa = 4.48 TDD142 pKa = 3.01 GGMLTTQTATAFTEE156 pKa = 5.22 DD157 pKa = 4.03 SFLLNDD163 pKa = 4.55 ASDD166 pKa = 4.03 TPLSEE171 pKa = 3.98 AAAAEE176 pKa = 4.16 ASAGEE181 pKa = 4.2 AEE183 pKa = 4.44 TEE185 pKa = 4.13 KK186 pKa = 11.32 GG187 pKa = 3.39
Molecular weight: 19.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.935
IPC_protein 3.884
Toseland 3.694
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.605
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.151
Thurlkill 3.732
EMBOSS 3.77
Sillero 3.999
Patrickios 1.036
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|R6LRL4|R6LRL4_9FIRM Membrane-associated phospholipid phosphatase OS=Firmicutes bacterium CAG:170 OX=1263006 GN=BN515_00570 PE=4 SV=1
MM1 pKa = 7.38 QRR3 pKa = 11.84 RR4 pKa = 11.84 NDD6 pKa = 3.46 LAGAIDD12 pKa = 3.82 IVVVAVDD19 pKa = 4.2 LDD21 pKa = 3.69 QARR24 pKa = 11.84 VGLDD28 pKa = 2.99 AVDD31 pKa = 4.01 VVVQLAVLVYY41 pKa = 10.44 NAILLFGIRR50 pKa = 11.84 FGIRR54 pKa = 11.84 FRR56 pKa = 11.84 IWIWRR61 pKa = 11.84 RR62 pKa = 11.84 LFDD65 pKa = 4.28 HH66 pKa = 6.57 LAIRR70 pKa = 11.84 AKK72 pKa = 10.59 VIEE75 pKa = 4.25 ARR77 pKa = 11.84 RR78 pKa = 11.84 KK79 pKa = 9.07 KK80 pKa = 10.16 PVGTIHH86 pKa = 7.45 PVMQRR91 pKa = 11.84 GNDD94 pKa = 3.36 LKK96 pKa = 11.11 GAVDD100 pKa = 3.44 IVIIALDD107 pKa = 4.11 LDD109 pKa = 3.56 QARR112 pKa = 11.84 IGRR115 pKa = 11.84 NAIHH119 pKa = 6.61 IVVKK123 pKa = 10.72 HH124 pKa = 6.02 SVDD127 pKa = 3.68 LNNSVIVRR135 pKa = 11.84 IRR137 pKa = 11.84 LKK139 pKa = 10.76 LQMSVLIEE147 pKa = 4.14 VIEE150 pKa = 4.49 CFRR153 pKa = 11.84 KK154 pKa = 7.68 TCVIRR159 pKa = 11.84 IGHH162 pKa = 6.22 PVAAVFNCCIIAGKK176 pKa = 10.08 IIVISVFCLIQAVTNHH192 pKa = 5.08 TVDD195 pKa = 4.35 RR196 pKa = 11.84 IVGISVQLKK205 pKa = 7.99 QSCMLINTLTNAILAEE221 pKa = 4.11 IVPISWIVVVVDD233 pKa = 4.49 FLHH236 pKa = 6.6 IAKK239 pKa = 10.23 ALIAIAVLCKK249 pKa = 10.28 VIKK252 pKa = 10.71 VSIDD256 pKa = 3.39 CKK258 pKa = 10.63 PLMIYY263 pKa = 10.08 IGRR266 pKa = 11.84 TKK268 pKa = 10.15 IISRR272 pKa = 11.84 AIICFQPRR280 pKa = 11.84 AVDD283 pKa = 3.38 QLAVLKK289 pKa = 10.74 LIGQICEE296 pKa = 3.61 EE297 pKa = 4.18 ALRR300 pKa = 11.84 LSIQALEE307 pKa = 3.99 ADD309 pKa = 3.69 RR310 pKa = 11.84 VEE312 pKa = 4.38 VIIAFLTVFIRR323 pKa = 11.84 QIPPAGLGHH332 pKa = 6.86 AVDD335 pKa = 4.5 GVIARR340 pKa = 11.84 AVQLDD345 pKa = 3.55 QARR348 pKa = 11.84 DD349 pKa = 3.44 LALTGAGHH357 pKa = 6.13 FVEE360 pKa = 5.69 LIEE363 pKa = 4.4 IFVSFVIQTGDD374 pKa = 4.14 LVDD377 pKa = 3.57 ALQRR381 pKa = 11.84 LIVDD385 pKa = 3.86 EE386 pKa = 4.57 VVGHH390 pKa = 6.27 AVDD393 pKa = 4.66 RR394 pKa = 11.84 FPAGPRR400 pKa = 11.84 AVVQNEE406 pKa = 3.85 AVGEE410 pKa = 4.03 ILIGFAVEE418 pKa = 3.66 MAAARR423 pKa = 11.84 MRR425 pKa = 11.84 GVFRR429 pKa = 11.84 LLIGVNAVLLLEE441 pKa = 4.93 GGLAGHH447 pKa = 6.24 AVEE450 pKa = 5.92 RR451 pKa = 11.84 IRR453 pKa = 11.84 AQIDD457 pKa = 3.56 VVADD461 pKa = 3.87 RR462 pKa = 11.84 AGVLDD467 pKa = 4.1 AQALIIIPLCAVLGGQLHH485 pKa = 6.22 AAEE488 pKa = 5.13 DD489 pKa = 4.06 ADD491 pKa = 4.0 GVGRR495 pKa = 11.84 IGIDD499 pKa = 3.31 GLTLLDD505 pKa = 4.89 KK506 pKa = 11.12 VALDD510 pKa = 3.53 GKK512 pKa = 10.6 KK513 pKa = 10.18 VVFVQHH519 pKa = 6.55 RR520 pKa = 11.84 RR521 pKa = 11.84 GQRR524 pKa = 11.84 EE525 pKa = 3.91 VIVRR529 pKa = 11.84 HH530 pKa = 5.35 RR531 pKa = 11.84 QTTDD535 pKa = 2.62 VHH537 pKa = 6.2 SHH539 pKa = 4.88 VLVKK543 pKa = 10.85 LEE545 pKa = 4.28 LYY547 pKa = 10.37 RR548 pKa = 11.84 HH549 pKa = 6.36 FGLFADD555 pKa = 5.03 ALRR558 pKa = 11.84 QLEE561 pKa = 4.02 QQIPCRR567 pKa = 11.84 DD568 pKa = 3.79 AGNVAAGNHH577 pKa = 4.89 LQQRR581 pKa = 11.84 LQLLRR586 pKa = 11.84 EE587 pKa = 4.37 LDD589 pKa = 3.32 RR590 pKa = 11.84 HH591 pKa = 5.65 HH592 pKa = 6.73 VQTEE596 pKa = 3.99 NVGNVHH602 pKa = 6.79 RR603 pKa = 11.84 LAGIDD608 pKa = 3.42 HH609 pKa = 7.23 MIAVDD614 pKa = 3.98 LAVAAGDD621 pKa = 3.65 VFRR624 pKa = 11.84 VRR626 pKa = 11.84 DD627 pKa = 3.44 LTRR630 pKa = 11.84 PCKK633 pKa = 10.34 RR634 pKa = 11.84 PVAACRR640 pKa = 11.84 VVKK643 pKa = 11.1 VEE645 pKa = 4.26 DD646 pKa = 3.47 RR647 pKa = 11.84 LIFAVHH653 pKa = 6.55 GDD655 pKa = 3.52 LDD657 pKa = 3.71 RR658 pKa = 11.84 RR659 pKa = 11.84 ADD661 pKa = 4.2 GIVAGNHH668 pKa = 5.45 CGHH671 pKa = 6.35 RR672 pKa = 11.84 RR673 pKa = 11.84 VFQSGAFHH681 pKa = 7.25 AGKK684 pKa = 10.12 CKK686 pKa = 10.43 AVTHH690 pKa = 6.08 SFNRR694 pKa = 11.84 TCRR697 pKa = 11.84 AAAQLKK703 pKa = 10.01 RR704 pKa = 11.84 DD705 pKa = 3.68 VLRR708 pKa = 11.84 ADD710 pKa = 3.69 RR711 pKa = 11.84 NGIFIVGSDD720 pKa = 3.16 QGKK723 pKa = 9.7 VYY725 pKa = 10.74 RR726 pKa = 11.84 LAIDD730 pKa = 5.34 GIDD733 pKa = 3.31 ARR735 pKa = 11.84 LGEE738 pKa = 4.4 FQAVRR743 pKa = 11.84 LDD745 pKa = 3.47 HH746 pKa = 6.22 VEE748 pKa = 4.15 RR749 pKa = 11.84 LAADD753 pKa = 3.59 DD754 pKa = 4.13 RR755 pKa = 11.84 LAGQEE760 pKa = 4.01 LHH762 pKa = 6.37 LHH764 pKa = 6.8 ASRR767 pKa = 11.84 FDD769 pKa = 3.34 AGKK772 pKa = 10.42 YY773 pKa = 9.7 AVLGDD778 pKa = 3.77 RR779 pKa = 11.84 ADD781 pKa = 3.69 GLVRR785 pKa = 11.84 RR786 pKa = 11.84 PPCDD790 pKa = 2.66 IRR792 pKa = 11.84 RR793 pKa = 11.84 KK794 pKa = 9.02 LRR796 pKa = 11.84 RR797 pKa = 11.84 AAGHH801 pKa = 6.77 ADD803 pKa = 2.85 AGGRR807 pKa = 11.84 KK808 pKa = 6.91 WKK810 pKa = 10.24 RR811 pKa = 11.84 RR812 pKa = 11.84 ADD814 pKa = 3.93 RR815 pKa = 11.84 QVVIGRR821 pKa = 11.84 GDD823 pKa = 3.06 QRR825 pKa = 11.84 MIEE828 pKa = 4.09 LRR830 pKa = 11.84 RR831 pKa = 11.84 DD832 pKa = 3.22 RR833 pKa = 11.84 RR834 pKa = 11.84 SGNDD838 pKa = 3.17 HH839 pKa = 6.15 QRR841 pKa = 11.84 GADD844 pKa = 3.54 GTLVAVGGAVYY855 pKa = 10.25 DD856 pKa = 4.21 RR857 pKa = 11.84 KK858 pKa = 10.32 RR859 pKa = 11.84 VAALLLGNEE868 pKa = 4.49 GGRR871 pKa = 11.84 AAAVQIDD878 pKa = 4.13 GSHH881 pKa = 6.88 AARR884 pKa = 11.84 VQHH887 pKa = 6.99 DD888 pKa = 3.91 LRR890 pKa = 11.84 QLFCAAACGEE900 pKa = 4.19 RR901 pKa = 11.84 LLTAVEE907 pKa = 3.97 HH908 pKa = 6.28 HH909 pKa = 6.44 QDD911 pKa = 3.21 NLALRR916 pKa = 11.84 RR917 pKa = 11.84 DD918 pKa = 3.65 ADD920 pKa = 3.58 AGAGMAAVIVVGRR933 pKa = 11.84 AVHH936 pKa = 6.08 NRR938 pKa = 11.84 LAVLDD943 pKa = 4.37 QIDD946 pKa = 3.55 AAADD950 pKa = 3.66 GLLDD954 pKa = 5.28 LILIGVVFTGAADD967 pKa = 3.29 HH968 pKa = 6.67 GRR970 pKa = 11.84 AILQDD975 pKa = 3.14 RR976 pKa = 11.84 KK977 pKa = 9.27 EE978 pKa = 4.18 VRR980 pKa = 11.84 RR981 pKa = 11.84 CAAVVFDD988 pKa = 4.18 TLHH991 pKa = 6.4 DD992 pKa = 3.72 QRR994 pKa = 11.84 AVGSAGRR1001 pKa = 11.84 HH1002 pKa = 3.57 VVEE1005 pKa = 4.23 VRR1007 pKa = 11.84 IHH1009 pKa = 6.56 ADD1011 pKa = 2.88 HH1012 pKa = 6.99 SIVMLHH1018 pKa = 6.83 IIRR1021 pKa = 11.84 RR1022 pKa = 11.84 IGRR1025 pKa = 11.84 IGMPLLGRR1033 pKa = 11.84 RR1034 pKa = 11.84 HH1035 pKa = 5.82 LVGDD1039 pKa = 3.72 TGHH1042 pKa = 6.96 RR1043 pKa = 11.84 PAEE1046 pKa = 3.76 RR1047 pKa = 11.84 RR1048 pKa = 11.84 IICVIVRR1055 pKa = 11.84 VDD1057 pKa = 3.1 VHH1059 pKa = 7.62 IVAGDD1064 pKa = 3.38 IDD1066 pKa = 4.49 RR1067 pKa = 11.84 GNVIDD1072 pKa = 5.49 DD1073 pKa = 4.28 LLIVLCQRR1081 pKa = 11.84 VVDD1084 pKa = 4.46 RR1085 pKa = 11.84 LRR1087 pKa = 11.84 HH1088 pKa = 5.4 AGNDD1092 pKa = 2.77 RR1093 pKa = 11.84 RR1094 pKa = 11.84 RR1095 pKa = 11.84 IRR1097 pKa = 11.84 RR1098 pKa = 11.84 EE1099 pKa = 3.47 HH1100 pKa = 6.62 GIVSIIRR1107 pKa = 11.84 VGFRR1111 pKa = 11.84 IGLRR1115 pKa = 11.84 VRR1117 pKa = 11.84 IDD1119 pKa = 2.95 RR1120 pKa = 11.84 VGAVRR1125 pKa = 11.84 VAGQIEE1131 pKa = 4.32 QIVRR1135 pKa = 11.84 KK1136 pKa = 10.12 GIAARR1141 pKa = 11.84 LAEE1144 pKa = 4.14 RR1145 pKa = 11.84 LIVGKK1150 pKa = 10.18 VGQRR1154 pKa = 11.84 IRR1156 pKa = 11.84 AFQRR1160 pKa = 11.84 TDD1162 pKa = 2.92 QGIPGISAQDD1172 pKa = 3.8 LAPDD1176 pKa = 4.09 TVLGDD1181 pKa = 3.93 HH1182 pKa = 6.95 PAEE1185 pKa = 4.15 AVGQEE1190 pKa = 3.54 IRR1192 pKa = 11.84 RR1193 pKa = 11.84 AAFIRR1198 pKa = 11.84 KK1199 pKa = 6.88 TSRR1202 pKa = 11.84 KK1203 pKa = 9.4 VILSEE1208 pKa = 3.86 RR1209 pKa = 11.84 AEE1211 pKa = 4.08 ASAAIHH1217 pKa = 5.91 LRR1219 pKa = 11.84 CEE1221 pKa = 3.69 IEE1223 pKa = 4.64 RR1224 pKa = 11.84 IHH1226 pKa = 7.4 IAIEE1230 pKa = 3.6 VGIAKK1235 pKa = 10.23 RR1236 pKa = 11.84 MIDD1239 pKa = 3.91 LIEE1242 pKa = 4.27 RR1243 pKa = 11.84 AVGHH1247 pKa = 6.88 AEE1249 pKa = 4.11 VSCPDD1254 pKa = 3.28 VGRR1257 pKa = 11.84 KK1258 pKa = 8.36 NVHH1261 pKa = 4.42 IRR1263 pKa = 11.84 LRR1265 pKa = 11.84 GVYY1268 pKa = 8.62 SVRR1271 pKa = 11.84 NVHH1274 pKa = 6.09 VLGAVHH1280 pKa = 6.01 QVGDD1284 pKa = 3.57 IAGPAAHH1291 pKa = 6.63 VGAGLYY1297 pKa = 10.69 GGFRR1301 pKa = 11.84 HH1302 pKa = 6.39 ALHH1305 pKa = 6.37 LRR1307 pKa = 11.84 VVRR1310 pKa = 11.84 HH1311 pKa = 4.76 IHH1313 pKa = 5.15 RR1314 pKa = 11.84 TEE1316 pKa = 3.63 HH1317 pKa = 5.22 MAEE1320 pKa = 3.97 VSRR1323 pKa = 11.84 IAVGQRR1329 pKa = 11.84 KK1330 pKa = 8.72 VLDD1333 pKa = 3.25 VLQRR1337 pKa = 11.84 RR1338 pKa = 11.84 RR1339 pKa = 11.84 RR1340 pKa = 11.84 GSVRR1344 pKa = 11.84 CVLLGNIGRR1353 pKa = 11.84 KK1354 pKa = 9.7 GCVFLAADD1362 pKa = 4.58 HH1363 pKa = 6.56 LPCAGRR1369 pKa = 11.84 VALNARR1375 pKa = 11.84 ADD1377 pKa = 3.79 EE1378 pKa = 4.38 VDD1380 pKa = 3.83 DD1381 pKa = 4.24 EE1382 pKa = 5.56 RR1383 pKa = 11.84 IDD1385 pKa = 3.41 VLARR1389 pKa = 11.84 IFRR1392 pKa = 11.84 RR1393 pKa = 11.84 VGLGVALQEE1402 pKa = 3.88 LQARR1406 pKa = 11.84 KK1407 pKa = 8.54 EE1408 pKa = 3.92 RR1409 pKa = 11.84 RR1410 pKa = 11.84 VVRR1413 pKa = 11.84 NRR1415 pKa = 11.84 LRR1417 pKa = 11.84 SRR1419 pKa = 11.84 RR1420 pKa = 11.84 LLLHH1424 pKa = 6.38 RR1425 pKa = 11.84 EE1426 pKa = 4.04 RR1427 pKa = 11.84 FIKK1430 pKa = 9.82 WLLRR1434 pKa = 11.84 KK1435 pKa = 9.88 LFLRR1439 pKa = 11.84 PGPDD1443 pKa = 2.87 ILLAGCKK1450 pKa = 9.63 RR1451 pKa = 11.84 LFVVLLHH1458 pKa = 6.41 IPARR1462 pKa = 11.84 LLVRR1466 pKa = 11.84 RR1467 pKa = 11.84 IRR1469 pKa = 11.84 GHH1471 pKa = 4.43 GQQRR1475 pKa = 11.84 RR1476 pKa = 11.84 QQQYY1480 pKa = 7.24 QDD1482 pKa = 3.0 EE1483 pKa = 4.4 YY1484 pKa = 10.99 RR1485 pKa = 11.84 RR1486 pKa = 11.84 YY1487 pKa = 10.18 ASFRR1491 pKa = 11.84 LSASHH1496 pKa = 6.51 VLFSFLLFQMIDD1508 pKa = 3.41 QIII1511 pKa = 3.6
Molecular weight: 167.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.355
IPC_protein 10.189
Toseland 10.687
ProMoST 10.482
Dawson 10.745
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.774
Grimsley 10.774
Solomon 10.935
Lehninger 10.891
Nozaki 10.701
DTASelect 10.482
Thurlkill 10.672
EMBOSS 11.111
Sillero 10.701
Patrickios 10.467
IPC_peptide 10.95
IPC2_peptide 9.809
IPC2.peptide.svr19 8.751
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2216
0
2216
674074
29
2520
304.2
33.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.999 ± 0.055
1.839 ± 0.027
5.613 ± 0.045
6.934 ± 0.051
3.826 ± 0.034
7.555 ± 0.053
1.928 ± 0.03
5.592 ± 0.047
4.739 ± 0.055
9.986 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.773 ± 0.031
3.265 ± 0.032
4.037 ± 0.032
3.467 ± 0.031
5.915 ± 0.059
5.556 ± 0.047
5.468 ± 0.041
6.929 ± 0.051
1.016 ± 0.019
3.56 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here