Amino acid dipepetide frequency for Firmicutes bacterium CAG:170

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.138AlaAla: 13.138 ± 0.232	1.748AlaCys: 1.748 ± 0.061	5.821AlaAsp: 5.821 ± 0.129	7.983AlaGlu: 7.983 ± 0.132	3.651AlaPhe: 3.651 ± 0.076	7.762AlaGly: 7.762 ± 0.145	1.712AlaHis: 1.712 ± 0.056	4.921AlaIle: 4.921 ± 0.096	4.516AlaLys: 4.516 ± 0.098	10.573AlaLeu: 10.573 ± 0.177	2.835AlaMet: 2.835 ± 0.073	2.5AlaAsn: 2.5 ± 0.07	3.572AlaPro: 3.572 ± 0.095	3.992AlaGln: 3.992 ± 0.084	4.774AlaArg: 4.774 ± 0.109	4.979AlaSer: 4.979 ± 0.103	3.461AlaThr: 3.461 ± 0.082	8.039AlaVal: 8.039 ± 0.156	0.926AlaTrp: 0.926 ± 0.039	3.081AlaTyr: 3.081 ± 0.08	0.006AlaXaa: 0.006 ± 0.003
Cys	2.058CysAla: 2.058 ± 0.059	0.43CysCys: 0.43 ± 0.033	1.004CysAsp: 1.004 ± 0.036	1.062CysGlu: 1.062 ± 0.04	0.687CysPhe: 0.687 ± 0.034	2.11CysGly: 2.11 ± 0.065	0.331CysHis: 0.331 ± 0.021	0.997CysIle: 0.997 ± 0.039	0.749CysLys: 0.749 ± 0.033	1.374CysLeu: 1.374 ± 0.048	0.469CysMet: 0.469 ± 0.024	0.5CysAsn: 0.5 ± 0.029	0.797CysPro: 0.797 ± 0.04	0.386CysGln: 0.386 ± 0.024	1.264CysArg: 1.264 ± 0.045	1.033CysSer: 1.033 ± 0.049	0.992CysThr: 0.992 ± 0.051	1.44CysVal: 1.44 ± 0.054	0.162CysTrp: 0.162 ± 0.017	0.543CysTyr: 0.543 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	6.29AspAla: 6.29 ± 0.118	1.03AspCys: 1.03 ± 0.036	2.921AspAsp: 2.921 ± 0.079	4.261AspGlu: 4.261 ± 0.088	2.34AspPhe: 2.34 ± 0.058	5.401AspGly: 5.401 ± 0.116	1.024AspHis: 1.024 ± 0.042	3.224AspIle: 3.224 ± 0.077	2.261AspLys: 2.261 ± 0.063	4.818AspLeu: 4.818 ± 0.115	1.503AspMet: 1.503 ± 0.05	1.438AspAsn: 1.438 ± 0.052	2.394AspPro: 2.394 ± 0.068	1.338AspGln: 1.338 ± 0.052	3.109AspArg: 3.109 ± 0.098	2.682AspSer: 2.682 ± 0.066	3.049AspThr: 3.049 ± 0.071	4.04AspVal: 4.04 ± 0.098	0.687AspTrp: 0.687 ± 0.038	2.325AspTyr: 2.325 ± 0.068	0.001AspXaa: 0.001 ± 0.001
Glu	7.042GluAla: 7.042 ± 0.123	0.825GluCys: 0.825 ± 0.037	3.261GluAsp: 3.261 ± 0.086	5.028GluGlu: 5.028 ± 0.115	2.144GluPhe: 2.144 ± 0.062	4.007GluGly: 4.007 ± 0.075	1.467GluHis: 1.467 ± 0.055	4.189GluIle: 4.189 ± 0.083	4.75GluLys: 4.75 ± 0.088	7.71GluLeu: 7.71 ± 0.118	2.068GluMet: 2.068 ± 0.06	3.466GluAsn: 3.466 ± 0.071	2.546GluPro: 2.546 ± 0.068	3.141GluGln: 3.141 ± 0.07	4.4GluArg: 4.4 ± 0.093	3.117GluSer: 3.117 ± 0.066	3.741GluThr: 3.741 ± 0.09	3.492GluVal: 3.492 ± 0.077	0.599GluTrp: 0.599 ± 0.029	2.341GluTyr: 2.341 ± 0.072	0.001GluXaa: 0.001 ± 0.001
Phe	3.703PheAla: 3.703 ± 0.079	0.862PheCys: 0.862 ± 0.035	2.515PheAsp: 2.515 ± 0.058	2.314PheGlu: 2.314 ± 0.063	1.663PhePhe: 1.663 ± 0.057	3.043PheGly: 3.043 ± 0.081	0.78PheHis: 0.78 ± 0.036	1.771PheIle: 1.771 ± 0.054	1.262PheLys: 1.262 ± 0.049	3.746PheLeu: 3.746 ± 0.098	0.875PheMet: 0.875 ± 0.039	1.104PheAsn: 1.104 ± 0.044	1.484PhePro: 1.484 ± 0.042	1.104PheGln: 1.104 ± 0.042	2.359PheArg: 2.359 ± 0.062	2.893PheSer: 2.893 ± 0.08	2.242PheThr: 2.242 ± 0.059	2.639PheVal: 2.639 ± 0.061	0.412PheTrp: 0.412 ± 0.025	1.485PheTyr: 1.485 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	6.655GlyAla: 6.655 ± 0.099	1.705GlyCys: 1.705 ± 0.054	3.848GlyAsp: 3.848 ± 0.101	4.937GlyGlu: 4.937 ± 0.089	3.072GlyPhe: 3.072 ± 0.085	5.915GlyGly: 5.915 ± 0.106	1.497GlyHis: 1.497 ± 0.052	4.719GlyIle: 4.719 ± 0.097	4.531GlyLys: 4.531 ± 0.095	6.605GlyLeu: 6.605 ± 0.113	2.383GlyMet: 2.383 ± 0.069	2.475GlyAsn: 2.475 ± 0.072	1.623GlyPro: 1.623 ± 0.059	2.224GlyGln: 2.224 ± 0.076	4.307GlyArg: 4.307 ± 0.113	4.405GlySer: 4.405 ± 0.101	4.589GlyThr: 4.589 ± 0.092	6.075GlyVal: 6.075 ± 0.122	0.9GlyTrp: 0.9 ± 0.04	3.087GlyTyr: 3.087 ± 0.079	0.001GlyXaa: 0.001 ± 0.001
His	1.71HisAla: 1.71 ± 0.059	0.393HisCys: 0.393 ± 0.026	1.185HisAsp: 1.185 ± 0.054	1.165HisGlu: 1.165 ± 0.045	0.865HisPhe: 0.865 ± 0.035	1.682HisGly: 1.682 ± 0.055	0.491HisHis: 0.491 ± 0.051	1.34HisIle: 1.34 ± 0.054	0.728HisLys: 0.728 ± 0.034	1.651HisLeu: 1.651 ± 0.054	0.55HisMet: 0.55 ± 0.03	0.59HisAsn: 0.59 ± 0.034	1.064HisPro: 1.064 ± 0.043	0.539HisGln: 0.539 ± 0.028	1.05HisArg: 1.05 ± 0.052	0.979HisSer: 0.979 ± 0.039	1.145HisThr: 1.145 ± 0.05	1.221HisVal: 1.221 ± 0.052	0.236HisTrp: 0.236 ± 0.018	0.691HisTyr: 0.691 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.548IleAla: 5.548 ± 0.096	1.2IleCys: 1.2 ± 0.044	3.452IleAsp: 3.452 ± 0.076	3.378IleGlu: 3.378 ± 0.076	2.071IlePhe: 2.071 ± 0.052	4.396IleGly: 4.396 ± 0.092	1.184IleHis: 1.184 ± 0.047	2.691IleIle: 2.691 ± 0.072	1.819IleLys: 1.819 ± 0.055	5.858IleLeu: 5.858 ± 0.111	1.319IleMet: 1.319 ± 0.043	1.623IleAsn: 1.623 ± 0.052	2.816IlePro: 2.816 ± 0.068	1.731IleGln: 1.731 ± 0.06	3.651IleArg: 3.651 ± 0.079	3.523IleSer: 3.523 ± 0.076	3.236IleThr: 3.236 ± 0.082	4.011IleVal: 4.011 ± 0.077	0.507IleTrp: 0.507 ± 0.027	1.899IleTyr: 1.899 ± 0.055	0.001IleXaa: 0.001 ± 0.001
Lys	4.333LysAla: 4.333 ± 0.091	0.586LysCys: 0.586 ± 0.033	2.446LysAsp: 2.446 ± 0.063	3.166LysGlu: 3.166 ± 0.081	1.357LysPhe: 1.357 ± 0.054	2.897LysGly: 2.897 ± 0.071	0.782LysHis: 0.782 ± 0.034	2.872LysIle: 2.872 ± 0.08	3.468LysLys: 3.468 ± 0.09	4.729LysLeu: 4.729 ± 0.079	1.51LysMet: 1.51 ± 0.054	2.188LysAsn: 2.188 ± 0.058	2.072LysPro: 2.072 ± 0.063	1.854LysGln: 1.854 ± 0.054	2.762LysArg: 2.762 ± 0.072	2.415LysSer: 2.415 ± 0.07	3.043LysThr: 3.043 ± 0.067	2.685LysVal: 2.685 ± 0.071	0.452LysTrp: 0.452 ± 0.027	1.771LysTyr: 1.771 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	9.469LeuAla: 9.469 ± 0.147	2.19LeuCys: 2.19 ± 0.066	5.671LeuAsp: 5.671 ± 0.1	6.268LeuGlu: 6.268 ± 0.115	3.839LeuPhe: 3.839 ± 0.085	6.838LeuGly: 6.838 ± 0.117	2.161LeuHis: 2.161 ± 0.071	5.034LeuIle: 5.034 ± 0.102	4.078LeuLys: 4.078 ± 0.082	10.934LeuLeu: 10.934 ± 0.173	2.589LeuMet: 2.589 ± 0.071	2.924LeuAsn: 2.924 ± 0.066	4.882LeuPro: 4.882 ± 0.104	3.115LeuGln: 3.115 ± 0.074	7.194LeuArg: 7.194 ± 0.154	6.606LeuSer: 6.606 ± 0.109	6.06LeuThr: 6.06 ± 0.108	5.814LeuVal: 5.814 ± 0.101	0.918LeuTrp: 0.918 ± 0.038	3.276LeuTyr: 3.276 ± 0.076	0.003LeuXaa: 0.003 ± 0.002
Met	2.537MetAla: 2.537 ± 0.063	0.36MetCys: 0.36 ± 0.025	1.608MetAsp: 1.608 ± 0.044	2.105MetGlu: 2.105 ± 0.059	0.792MetPhe: 0.792 ± 0.037	1.789MetGly: 1.789 ± 0.055	0.487MetHis: 0.487 ± 0.027	1.406MetIle: 1.406 ± 0.04	1.768MetLys: 1.768 ± 0.055	2.974MetLeu: 2.974 ± 0.076	0.765MetMet: 0.765 ± 0.039	1.102MetAsn: 1.102 ± 0.04	1.242MetPro: 1.242 ± 0.049	1.28MetGln: 1.28 ± 0.043	1.604MetArg: 1.604 ± 0.051	1.506MetSer: 1.506 ± 0.045	1.814MetThr: 1.814 ± 0.051	1.641MetVal: 1.641 ± 0.059	0.191MetTrp: 0.191 ± 0.019	0.757MetTyr: 0.757 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.442AsnAla: 3.442 ± 0.075	0.509AsnCys: 0.509 ± 0.025	1.725AsnAsp: 1.725 ± 0.048	1.929AsnGlu: 1.929 ± 0.058	1.197AsnPhe: 1.197 ± 0.044	3.289AsnGly: 3.289 ± 0.076	0.616AsnHis: 0.616 ± 0.028	2.031AsnIle: 2.031 ± 0.053	1.271AsnLys: 1.271 ± 0.044	2.866AsnLeu: 2.866 ± 0.073	0.949AsnMet: 0.949 ± 0.033	1.089AsnAsn: 1.089 ± 0.045	1.544AsnPro: 1.544 ± 0.056	0.958AsnGln: 0.958 ± 0.042	1.736AsnArg: 1.736 ± 0.054	1.528AsnSer: 1.528 ± 0.053	1.808AsnThr: 1.808 ± 0.06	2.556AsnVal: 2.556 ± 0.057	0.375AsnTrp: 0.375 ± 0.023	1.227AsnTyr: 1.227 ± 0.045	0.001AsnXaa: 0.001 ± 0.002
Pro	4.191ProAla: 4.191 ± 0.092	0.629ProCys: 0.629 ± 0.036	2.937ProAsp: 2.937 ± 0.07	4.6ProGlu: 4.6 ± 0.081	1.561ProPhe: 1.561 ± 0.042	2.911ProGly: 2.911 ± 0.068	0.728ProHis: 0.728 ± 0.033	1.837ProIle: 1.837 ± 0.059	1.807ProLys: 1.807 ± 0.06	3.319ProLeu: 3.319 ± 0.08	1.037ProMet: 1.037 ± 0.039	1.2ProAsn: 1.2 ± 0.043	1.237ProPro: 1.237 ± 0.051	1.651ProGln: 1.651 ± 0.051	1.584ProArg: 1.584 ± 0.057	2.16ProSer: 2.16 ± 0.067	1.822ProThr: 1.822 ± 0.054	3.314ProVal: 3.314 ± 0.077	0.429ProTrp: 0.429 ± 0.03	1.403ProTyr: 1.403 ± 0.043	0.007ProXaa: 0.007 ± 0.003
Gln	3.333GlnAla: 3.333 ± 0.079	0.494GlnCys: 0.494 ± 0.026	1.574GlnAsp: 1.574 ± 0.049	2.5GlnGlu: 2.5 ± 0.069	1.102GlnPhe: 1.102 ± 0.04	1.722GlnGly: 1.722 ± 0.054	0.619GlnHis: 0.619 ± 0.035	2.296GlnIle: 2.296 ± 0.064	2.265GlnLys: 2.265 ± 0.059	3.62GlnLeu: 3.62 ± 0.09	1.102GlnMet: 1.102 ± 0.041	1.466GlnAsn: 1.466 ± 0.05	1.534GlnPro: 1.534 ± 0.05	1.489GlnGln: 1.489 ± 0.054	2.139GlnArg: 2.139 ± 0.084	1.866GlnSer: 1.866 ± 0.053	2.104GlnThr: 2.104 ± 0.061	1.789GlnVal: 1.789 ± 0.052	0.334GlnTrp: 0.334 ± 0.025	1.325GlnTyr: 1.325 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	5.16ArgAla: 5.16 ± 0.13	1.055ArgCys: 1.055 ± 0.043	2.921ArgAsp: 2.921 ± 0.083	4.413ArgGlu: 4.413 ± 0.088	2.482ArgPhe: 2.482 ± 0.066	3.51ArgGly: 3.51 ± 0.09	1.191ArgHis: 1.191 ± 0.055	3.744ArgIle: 3.744 ± 0.089	3.02ArgLys: 3.02 ± 0.075	6.151ArgLeu: 6.151 ± 0.139	1.78ArgMet: 1.78 ± 0.053	1.829ArgAsn: 1.829 ± 0.052	2.173ArgPro: 2.173 ± 0.06	2.427ArgGln: 2.427 ± 0.083	4.473ArgArg: 4.473 ± 0.133	3.191ArgSer: 3.191 ± 0.084	3.089ArgThr: 3.089 ± 0.081	3.789ArgVal: 3.789 ± 0.102	0.616ArgTrp: 0.616 ± 0.034	2.132ArgTyr: 2.132 ± 0.059	0.0ArgXaa: 0.0 ± 0.0
Ser	5.763SerAla: 5.763 ± 0.097	1.083SerCys: 1.083 ± 0.044	3.172SerAsp: 3.172 ± 0.071	3.609SerGlu: 3.609 ± 0.08	2.483SerPhe: 2.483 ± 0.073	5.461SerGly: 5.461 ± 0.111	0.93SerHis: 0.93 ± 0.036	3.286SerIle: 3.286 ± 0.077	2.193SerLys: 2.193 ± 0.067	4.814SerLeu: 4.814 ± 0.085	1.568SerMet: 1.568 ± 0.055	1.59SerAsn: 1.59 ± 0.047	1.932SerPro: 1.932 ± 0.064	1.613SerGln: 1.613 ± 0.056	3.206SerArg: 3.206 ± 0.076	3.007SerSer: 3.007 ± 0.087	2.678SerThr: 2.678 ± 0.068	4.486SerVal: 4.486 ± 0.089	0.599SerTrp: 0.599 ± 0.032	2.084SerTyr: 2.084 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	5.949ThrAla: 5.949 ± 0.107	0.774ThrCys: 0.774 ± 0.038	3.314ThrAsp: 3.314 ± 0.075	3.523ThrGlu: 3.523 ± 0.088	1.992ThrPhe: 1.992 ± 0.059	4.74ThrGly: 4.74 ± 0.095	0.945ThrHis: 0.945 ± 0.036	3.188ThrIle: 3.188 ± 0.08	2.11ThrLys: 2.11 ± 0.061	5.75ThrLeu: 5.75 ± 0.099	1.396ThrMet: 1.396 ± 0.044	1.58ThrAsn: 1.58 ± 0.051	2.826ThrPro: 2.826 ± 0.069	1.811ThrGln: 1.811 ± 0.053	2.538ThrArg: 2.538 ± 0.058	2.519ThrSer: 2.519 ± 0.063	2.706ThrThr: 2.706 ± 0.074	4.715ThrVal: 4.715 ± 0.102	0.537ThrTrp: 0.537 ± 0.031	1.768ThrTyr: 1.768 ± 0.064	0.0ThrXaa: 0.0 ± 0.0
Val	5.412ValAla: 5.412 ± 0.109	1.596ValCys: 1.596 ± 0.048	3.79ValAsp: 3.79 ± 0.093	4.244ValGlu: 4.244 ± 0.083	3.006ValPhe: 3.006 ± 0.07	4.618ValGly: 4.618 ± 0.105	1.403ValHis: 1.403 ± 0.062	3.872ValIle: 3.872 ± 0.077	3.058ValLys: 3.058 ± 0.079	7.653ValLeu: 7.653 ± 0.129	1.902ValMet: 1.902 ± 0.057	2.301ValAsn: 2.301 ± 0.057	2.885ValPro: 2.885 ± 0.063	2.383ValGln: 2.383 ± 0.066	4.232ValArg: 4.232 ± 0.104	4.59ValSer: 4.59 ± 0.089	4.375ValThr: 4.375 ± 0.104	4.802ValVal: 4.802 ± 0.102	0.783ValTrp: 0.783 ± 0.038	2.38ValTyr: 2.38 ± 0.068	0.001ValXaa: 0.001 ± 0.002
Trp	0.792TrpAla: 0.792 ± 0.039	0.22TrpCys: 0.22 ± 0.018	0.579TrpAsp: 0.579 ± 0.033	0.641TrpGlu: 0.641 ± 0.033	0.445TrpPhe: 0.445 ± 0.025	0.694TrpGly: 0.694 ± 0.038	0.206TrpHis: 0.206 ± 0.019	0.552TrpIle: 0.552 ± 0.031	0.541TrpLys: 0.541 ± 0.027	1.142TrpLeu: 1.142 ± 0.048	0.301TrpMet: 0.301 ± 0.02	0.442TrpAsn: 0.442 ± 0.024	0.283TrpPro: 0.283 ± 0.024	0.45TrpGln: 0.45 ± 0.028	0.571TrpArg: 0.571 ± 0.033	0.625TrpSer: 0.625 ± 0.035	0.582TrpThr: 0.582 ± 0.036	0.528TrpVal: 0.528 ± 0.029	0.128TrpTrp: 0.128 ± 0.016	0.435TrpTyr: 0.435 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.458TyrAla: 3.458 ± 0.084	0.699TyrCys: 0.699 ± 0.034	2.388TyrAsp: 2.388 ± 0.067	2.209TyrGlu: 2.209 ± 0.061	1.506TyrPhe: 1.506 ± 0.048	2.762TyrGly: 2.762 ± 0.069	0.72TyrHis: 0.72 ± 0.034	1.943TyrIle: 1.943 ± 0.059	1.196TyrLys: 1.196 ± 0.049	3.574TyrLeu: 3.574 ± 0.077	0.826TyrMet: 0.826 ± 0.036	1.242TyrAsn: 1.242 ± 0.043	1.455TyrPro: 1.455 ± 0.05	1.188TyrGln: 1.188 ± 0.046	2.19TyrArg: 2.19 ± 0.058	1.935TyrSer: 1.935 ± 0.064	2.147TyrThr: 2.147 ± 0.067	2.21TyrVal: 2.21 ± 0.066	0.36TyrTrp: 0.36 ± 0.027	1.587TyrTyr: 1.587 ± 0.058	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.004XaaAla: 0.004 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.002	0.003XaaHis: 0.003 ± 0.002	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.003XaaArg: 0.003 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.003XaaTrp: 0.003 ± 0.002	0.0XaaTyr: 0.0 ± 0.0	0.028XaaXaa: 0.028 ± 0.009

Statistics based on 2216 proteins (674074 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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