Nanobsidianus stetteri

Taxonomy: cellular organisms; Archaea; DPANN group; Nanoarchaeota; Nanoarchaeales; Nanopusillaceae; Candidatus Nanobsidianus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 647 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R1FUB7|R1FUB7_NANST Uncharacterized protein OS=Nanobsidianus stetteri OX=1294122 GN=Nst1_049 PE=4 SV=1
MM1 pKa = 7.8DD2 pKa = 4.08VDD4 pKa = 4.14GLLDD8 pKa = 5.06AIVASIFLIIVSLIGIYY25 pKa = 10.19FPYY28 pKa = 9.84IVARR32 pKa = 11.84IFGTHH37 pKa = 5.53NPYY40 pKa = 10.75LILEE44 pKa = 4.25VAGFITYY51 pKa = 7.62STSEE55 pKa = 4.38PGNMTAAFLVTNAPDD70 pKa = 3.65FFYY73 pKa = 10.24EE74 pKa = 3.98FRR76 pKa = 11.84IIPPIQSSALFIPLYY91 pKa = 10.34NNNDD95 pKa = 3.42FLVSIGEE102 pKa = 4.47CYY104 pKa = 10.82VNNLWQQLADD114 pKa = 3.73DD115 pKa = 4.99ALNSFLTEE123 pKa = 3.92VFSYY127 pKa = 11.1LPGLKK132 pKa = 10.16NGVITKK138 pKa = 9.7PLADD142 pKa = 3.62VLNDD146 pKa = 3.39VFKK149 pKa = 11.13SSSQIAKK156 pKa = 7.96QTAIGAAGMYY166 pKa = 10.42GLYY169 pKa = 9.74TYY171 pKa = 10.94QSTLLQYY178 pKa = 10.67TSGNYY183 pKa = 9.84NIWGDD188 pKa = 3.59IFNALQSSVGPTLWSTVEE206 pKa = 3.72FTIMQVGDD214 pKa = 4.19MILDD218 pKa = 3.61EE219 pKa = 4.75TLPPPADD226 pKa = 3.76FVAGFAFNLAFTSAITLYY244 pKa = 10.51QYY246 pKa = 9.83WQDD249 pKa = 3.02TSNPYY254 pKa = 10.24ASCYY258 pKa = 9.62YY259 pKa = 10.66NQFIQQNTKK268 pKa = 10.29FLFLSIPNGEE278 pKa = 4.16YY279 pKa = 9.57TVYY282 pKa = 10.48QPYY285 pKa = 8.74LTLTIYY291 pKa = 10.42YY292 pKa = 9.57NPNFTFYY299 pKa = 10.74NGCNDD304 pKa = 3.74YY305 pKa = 10.69YY306 pKa = 11.53LCDD309 pKa = 3.25TSFTYY314 pKa = 9.94IYY316 pKa = 10.26DD317 pKa = 4.05PQDD320 pKa = 3.28NSQDD324 pKa = 3.83NIFKK328 pKa = 10.68LDD330 pKa = 4.06LGNVDD335 pKa = 3.64NNGNQVFPEE344 pKa = 4.55FIILSKK350 pKa = 11.17VGMQSGGPYY359 pKa = 10.24LNVSILSSTLSS370 pKa = 3.24

Molecular weight: 41.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R1G363|R1G363_NANST Uncharacterized protein OS=Nanobsidianus stetteri OX=1294122 GN=Nst1_231 PE=4 SV=1
MM1 pKa = 7.12TRR3 pKa = 11.84LGGTNKK9 pKa = 9.43VNHH12 pKa = 6.51VICRR16 pKa = 11.84RR17 pKa = 11.84CGRR20 pKa = 11.84KK21 pKa = 7.72TYY23 pKa = 10.84NPDD26 pKa = 2.78KK27 pKa = 10.43GYY29 pKa = 10.69CSHH32 pKa = 7.13CGFGRR37 pKa = 11.84SSKK40 pKa = 9.74IRR42 pKa = 11.84AYY44 pKa = 9.17VWSYY48 pKa = 10.06KK49 pKa = 10.07FKK51 pKa = 10.98RR52 pKa = 11.84KK53 pKa = 7.18WW54 pKa = 3.02

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

647

0

647

178873

31

1299

276.5

32.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.67 ± 0.083

0.591 ± 0.033

4.968 ± 0.093

7.237 ± 0.148

4.746 ± 0.071

5.168 ± 0.088

0.985 ± 0.037

12.505 ± 0.126

9.516 ± 0.183

10.085 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.62 ± 0.039

7.661 ± 0.173

3.16 ± 0.069

2.283 ± 0.086

3.393 ± 0.097

5.942 ± 0.099

3.893 ± 0.102

5.059 ± 0.092

0.782 ± 0.027

6.737 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski