Sinorhizobium sp. RAC02

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium; unclassified Sinorhizobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6245 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3M821|A0A1B3M821_9RHIZ DoxX family protein OS=Sinorhizobium sp. RAC02 OX=1842534 GN=BSY16_1827 PE=4 SV=1
MM1 pKa = 7.72PGYY4 pKa = 9.27STVEE8 pKa = 4.03GNDD11 pKa = 3.41FANVIVGQSWVVTNWQTGAGYY32 pKa = 10.96YY33 pKa = 10.29DD34 pKa = 3.6DD35 pKa = 5.91NYY37 pKa = 10.74WFDD40 pKa = 3.66TIYY43 pKa = 10.78GYY45 pKa = 11.13GGNDD49 pKa = 3.1KK50 pKa = 11.08LYY52 pKa = 11.1GLGGNDD58 pKa = 3.17RR59 pKa = 11.84LYY61 pKa = 11.28GGDD64 pKa = 4.63GVDD67 pKa = 3.47EE68 pKa = 4.83LYY70 pKa = 10.9GGKK73 pKa = 9.7GHH75 pKa = 7.25DD76 pKa = 4.02QLNGGAGNDD85 pKa = 3.5ILNGDD90 pKa = 4.27EE91 pKa = 5.08GDD93 pKa = 5.27DD94 pKa = 3.59ILSGGGGYY102 pKa = 10.08DD103 pKa = 3.25QLFGGAGWDD112 pKa = 3.87TFWGVDD118 pKa = 3.43GHH120 pKa = 6.74SSVMWGGAGADD131 pKa = 3.55KK132 pKa = 10.65MFGGSGSEE140 pKa = 3.9EE141 pKa = 4.37MYY143 pKa = 11.09GGDD146 pKa = 4.76DD147 pKa = 4.09ADD149 pKa = 3.75TMRR152 pKa = 11.84GSFGIDD158 pKa = 2.8TMSGDD163 pKa = 3.69NGHH166 pKa = 7.51DD167 pKa = 3.72DD168 pKa = 3.73MQGGGDD174 pKa = 4.24NDD176 pKa = 4.05TMSGGLGNDD185 pKa = 5.18LIAGDD190 pKa = 4.7AGADD194 pKa = 3.5TIFGNEE200 pKa = 3.62GRR202 pKa = 11.84DD203 pKa = 3.68NLSGGSGNDD212 pKa = 3.91TIWGDD217 pKa = 3.34GAYY220 pKa = 10.38FIAEE224 pKa = 3.93SNYY227 pKa = 8.62YY228 pKa = 10.36HH229 pKa = 7.19NDD231 pKa = 3.25IIDD234 pKa = 3.84GGTGNDD240 pKa = 3.74IIYY243 pKa = 10.59GGIGLDD249 pKa = 3.79TIKK252 pKa = 10.93GGTGRR257 pKa = 11.84DD258 pKa = 3.26TVYY261 pKa = 10.97GGTGDD266 pKa = 4.97DD267 pKa = 4.96VIDD270 pKa = 4.6SSDD273 pKa = 3.45GDD275 pKa = 3.75GGLVLILGADD285 pKa = 3.4DD286 pKa = 3.95SFYY289 pKa = 11.56GEE291 pKa = 5.25DD292 pKa = 4.27GNDD295 pKa = 3.42TIYY298 pKa = 11.13GRR300 pKa = 11.84NGNDD304 pKa = 3.13FLYY307 pKa = 10.8GGNGNDD313 pKa = 3.93YY314 pKa = 10.97LSGGQGSDD322 pKa = 3.39RR323 pKa = 11.84LSGDD327 pKa = 3.08AGFDD331 pKa = 3.4TIYY334 pKa = 10.92AGTGNDD340 pKa = 3.4TMDD343 pKa = 4.17GGSEE347 pKa = 3.87NDD349 pKa = 3.23NLYY352 pKa = 11.07GEE354 pKa = 5.05EE355 pKa = 4.29GADD358 pKa = 3.89SIRR361 pKa = 11.84GGTGNDD367 pKa = 3.31NVNGGAGNDD376 pKa = 4.19ALWGDD381 pKa = 3.85AGNDD385 pKa = 3.47GVYY388 pKa = 10.55GKK390 pKa = 10.7EE391 pKa = 4.3GDD393 pKa = 3.77DD394 pKa = 4.43QIWGGDD400 pKa = 3.69GNDD403 pKa = 5.13DD404 pKa = 3.2MWGDD408 pKa = 3.71AGNDD412 pKa = 3.17ILRR415 pKa = 11.84GGIGDD420 pKa = 4.61DD421 pKa = 3.52VLHH424 pKa = 6.85GNDD427 pKa = 4.12GDD429 pKa = 5.18DD430 pKa = 4.98ILFGDD435 pKa = 3.75QGTNTIYY442 pKa = 11.13GEE444 pKa = 4.1NGNDD448 pKa = 3.05RR449 pKa = 11.84FIASDD454 pKa = 5.15AIDD457 pKa = 4.05HH458 pKa = 6.3MNGGADD464 pKa = 3.39VDD466 pKa = 4.18TLSFSAYY473 pKa = 10.01LSVAVSVDD481 pKa = 3.61LAAGKK486 pKa = 10.29GLAGQALGDD495 pKa = 3.74TYY497 pKa = 11.62VGIEE501 pKa = 4.13NVVGGWGGDD510 pKa = 3.19RR511 pKa = 11.84LVGSAVANKK520 pKa = 10.4LLGEE524 pKa = 4.65KK525 pKa = 10.91GNDD528 pKa = 3.58TIFGGGGNDD537 pKa = 3.16RR538 pKa = 11.84LEE540 pKa = 4.95GGDD543 pKa = 3.38GHH545 pKa = 7.97DD546 pKa = 5.34GIRR549 pKa = 11.84GDD551 pKa = 4.16AGNDD555 pKa = 3.38TLYY558 pKa = 11.33GGAGNDD564 pKa = 3.49ALRR567 pKa = 11.84GGVGQDD573 pKa = 2.67IVFGGSGADD582 pKa = 3.09VFFYY586 pKa = 11.25VSLDD590 pKa = 3.47EE591 pKa = 4.7SGITAATRR599 pKa = 11.84DD600 pKa = 3.32IVRR603 pKa = 11.84DD604 pKa = 4.32FSRR607 pKa = 11.84AEE609 pKa = 3.76GDD611 pKa = 3.73RR612 pKa = 11.84INLSGIDD619 pKa = 3.59ASEE622 pKa = 4.03TANGNQAFTFRR633 pKa = 11.84GTNAFTGAAGEE644 pKa = 4.12LRR646 pKa = 11.84YY647 pKa = 7.71TASGGDD653 pKa = 3.67VYY655 pKa = 11.71VSGDD659 pKa = 3.29TDD661 pKa = 3.45GDD663 pKa = 3.63KK664 pKa = 11.27AADD667 pKa = 3.86FSILFDD673 pKa = 4.98NITSLAASDD682 pKa = 4.12FLLL685 pKa = 5.0

Molecular weight: 69.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3MBC2|A0A1B3MBC2_9RHIZ Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO OS=Sinorhizobium sp. RAC02 OX=1842534 GN=trmFO PE=3 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84KK3 pKa = 8.97FVLVSIALATAIGSFATPSLAQKK26 pKa = 10.19SGRR29 pKa = 11.84GDD31 pKa = 3.46NGNDD35 pKa = 3.12RR36 pKa = 11.84SDD38 pKa = 3.47RR39 pKa = 11.84GSSGRR44 pKa = 11.84GEE46 pKa = 4.27YY47 pKa = 10.4YY48 pKa = 9.02RR49 pKa = 11.84TVEE52 pKa = 4.25RR53 pKa = 11.84EE54 pKa = 3.83PFTHH58 pKa = 5.37VRR60 pKa = 11.84KK61 pKa = 9.13KK62 pKa = 10.42HH63 pKa = 5.2RR64 pKa = 11.84VILANSGAYY73 pKa = 10.18CSTNWAVLFDD83 pKa = 4.24RR84 pKa = 11.84SGHH87 pKa = 4.31RR88 pKa = 11.84TKK90 pKa = 10.48VRR92 pKa = 11.84HH93 pKa = 6.07CDD95 pKa = 3.09DD96 pKa = 4.35RR97 pKa = 11.84LPIDD101 pKa = 3.45VRR103 pKa = 3.81

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6245

0

6245

1946794

29

2876

311.7

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.224 ± 0.04

0.79 ± 0.009

5.725 ± 0.027

5.749 ± 0.029

3.95 ± 0.023

8.552 ± 0.031

2.003 ± 0.014

5.571 ± 0.025

3.597 ± 0.025

10.035 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.016

2.767 ± 0.02

4.805 ± 0.02

2.926 ± 0.018

6.653 ± 0.031

5.533 ± 0.023

5.541 ± 0.022

7.477 ± 0.026

1.28 ± 0.013

2.295 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski