Ustilaginoidea virens RNA virus 3
Average proteome isoelectric point is 7.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5TYK1|W5TYK1_9VIRU Coat protein OS=Ustilaginoidea virens RNA virus 3 OX=1460374 PE=4 SV=1
MM1 pKa = 7.32 ATTSRR6 pKa = 11.84 FQEE9 pKa = 3.99 NNFSYY14 pKa = 10.95 LSGGVAGYY22 pKa = 10.47 SSSTLVDD29 pKa = 3.44 DD30 pKa = 3.75 ATYY33 pKa = 10.36 RR34 pKa = 11.84 RR35 pKa = 11.84 YY36 pKa = 9.99 RR37 pKa = 11.84 AGLTIGTYY45 pKa = 7.53 EE46 pKa = 4.26 HH47 pKa = 7.1 GSYY50 pKa = 9.74 TYY52 pKa = 10.18 SRR54 pKa = 11.84 RR55 pKa = 11.84 SIFYY59 pKa = 8.72 EE60 pKa = 3.92 VGRR63 pKa = 11.84 HH64 pKa = 4.73 FSGVTKK70 pKa = 10.73 ALAYY74 pKa = 10.58 NHH76 pKa = 7.22 ADD78 pKa = 3.72 DD79 pKa = 5.96 DD80 pKa = 4.28 ALEE83 pKa = 4.64 IDD85 pKa = 4.09 ASIPINSVEE94 pKa = 3.92 AANFEE99 pKa = 3.95 GWARR103 pKa = 11.84 KK104 pKa = 9.44 YY105 pKa = 11.34 SNFSPQWHH113 pKa = 6.27 MMDD116 pKa = 3.31 LCAVVEE122 pKa = 4.21 RR123 pKa = 11.84 LGKK126 pKa = 10.12 AVAAQSVFGGVTTAHH141 pKa = 6.35 LRR143 pKa = 11.84 GGQPIRR149 pKa = 11.84 AVALGTLDD157 pKa = 3.78 SPQTASTNSVFIPRR171 pKa = 11.84 TVDD174 pKa = 3.12 SVGNDD179 pKa = 2.8 KK180 pKa = 10.96 VFSVLAAAVNGEE192 pKa = 4.09 GSTVTTDD199 pKa = 3.68 VLRR202 pKa = 11.84 LDD204 pKa = 4.07 AATNSPIMPEE214 pKa = 4.11 CSGHH218 pKa = 6.3 GLAKK222 pKa = 10.49 ACVEE226 pKa = 4.02 ALRR229 pKa = 11.84 VLGANFEE236 pKa = 4.22 ASGAGDD242 pKa = 4.13 LFAYY246 pKa = 10.43 ALTRR250 pKa = 11.84 GIHH253 pKa = 5.75 SGVSVVAHH261 pKa = 6.03 TDD263 pKa = 2.89 EE264 pKa = 5.22 GGWFRR269 pKa = 11.84 NVLRR273 pKa = 11.84 ACHH276 pKa = 6.02 FRR278 pKa = 11.84 PPYY281 pKa = 10.61 GGINLANRR289 pKa = 11.84 DD290 pKa = 4.03 YY291 pKa = 11.44 PFLPPLAAEE300 pKa = 4.86 DD301 pKa = 4.2 GGATSAWVDD310 pKa = 4.04 SIALKK315 pKa = 9.23 TAALVAHH322 pKa = 7.21 CDD324 pKa = 3.59 PCTHH328 pKa = 6.5 ATGGAYY334 pKa = 7.43 PTIFVANDD342 pKa = 3.55 GAVQPVGTHH351 pKa = 5.81 EE352 pKa = 4.14 PAGTEE357 pKa = 3.46 RR358 pKa = 11.84 DD359 pKa = 3.58 AASLGGQIAADD370 pKa = 3.58 VSRR373 pKa = 11.84 FAPLYY378 pKa = 9.33 MRR380 pKa = 11.84 GLVKK384 pKa = 10.81 LFGLRR389 pKa = 11.84 ANSGVAEE396 pKa = 4.27 SHH398 pKa = 6.65 FSTVANVTLSGTKK411 pKa = 9.74 DD412 pKa = 2.4 RR413 pKa = 11.84 HH414 pKa = 5.65 LRR416 pKa = 11.84 HH417 pKa = 5.83 KK418 pKa = 9.21 TVAPYY423 pKa = 9.74 FFIEE427 pKa = 4.14 PTSLIEE433 pKa = 4.18 HH434 pKa = 7.1 RR435 pKa = 11.84 FLGSPAEE442 pKa = 4.06 EE443 pKa = 4.25 AGFGALTSPGAEE455 pKa = 3.8 TEE457 pKa = 4.02 LATFEE462 pKa = 4.39 RR463 pKa = 11.84 VRR465 pKa = 11.84 EE466 pKa = 4.01 LDD468 pKa = 3.24 RR469 pKa = 11.84 GKK471 pKa = 9.89 HH472 pKa = 6.04 ANFCTIAFKK481 pKa = 10.48 MRR483 pKa = 11.84 TARR486 pKa = 11.84 TSGLVAAYY494 pKa = 9.93 AGTPAEE500 pKa = 4.03 LAGLRR505 pKa = 11.84 LYY507 pKa = 10.78 QFDD510 pKa = 3.92 EE511 pKa = 4.83 ASVVLPGDD519 pKa = 4.38 LGPTAGTVSAKK530 pKa = 10.03 HH531 pKa = 6.07 ANADD535 pKa = 3.87 PLSSYY540 pKa = 9.4 LWKK543 pKa = 10.42 RR544 pKa = 11.84 GQSPIPAPAEE554 pKa = 3.71 FVNIQGSYY562 pKa = 7.49 AAKK565 pKa = 8.91 YY566 pKa = 9.95 HH567 pKa = 5.33 VVTWDD572 pKa = 3.55 DD573 pKa = 4.54 DD574 pKa = 3.99 FDD576 pKa = 4.09 ATVSDD581 pKa = 5.47 LPEE584 pKa = 4.07 AWEE587 pKa = 4.52 LEE589 pKa = 4.18 SHH591 pKa = 5.03 PTKK594 pKa = 10.23 WRR596 pKa = 11.84 VSVPTAIRR604 pKa = 11.84 PGATNASDD612 pKa = 3.11 NGARR616 pKa = 11.84 RR617 pKa = 11.84 ARR619 pKa = 11.84 CRR621 pKa = 11.84 AAIALAQTVIRR632 pKa = 11.84 SRR634 pKa = 11.84 GLGDD638 pKa = 4.13 ANSPCITVSNTPPSWDD654 pKa = 4.37 DD655 pKa = 3.16 PVQPMARR662 pKa = 11.84 KK663 pKa = 9.52 EE664 pKa = 4.09 DD665 pKa = 4.06 TLHH668 pKa = 6.41 HH669 pKa = 6.77 PGVGTVSSFAEE680 pKa = 4.72 GNPPPAVPAALGPPLAPTLTHH701 pKa = 5.89 QPLRR705 pKa = 11.84 GAPLPRR711 pKa = 11.84 HH712 pKa = 5.83 GGGVVTGPPPTAAVDD727 pKa = 4.03 TPVVAPSAPPSVDD740 pKa = 3.47 PRR742 pKa = 11.84 PDD744 pKa = 3.72 DD745 pKa = 5.03 ADD747 pKa = 3.66 TATAGPAPRR756 pKa = 11.84 II757 pKa = 3.93
Molecular weight: 79.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.842
IPC2_protein 5.741
IPC_protein 5.817
Toseland 6.287
ProMoST 6.211
Dawson 6.122
Bjellqvist 6.122
Wikipedia 6.148
Rodwell 6.109
Grimsley 6.478
Solomon 6.122
Lehninger 6.122
Nozaki 6.402
DTASelect 6.59
Thurlkill 6.62
EMBOSS 6.576
Sillero 6.504
Patrickios 4.101
IPC_peptide 6.135
IPC2_peptide 6.517
IPC2.peptide.svr19 6.399
Protein with the highest isoelectric point:
>tr|W5TYK1|W5TYK1_9VIRU Coat protein OS=Ustilaginoidea virens RNA virus 3 OX=1460374 PE=4 SV=1
MM1 pKa = 7.73 GDD3 pKa = 4.07 DD4 pKa = 4.75 VITDD8 pKa = 3.88 RR9 pKa = 11.84 ANKK12 pKa = 9.88 HH13 pKa = 5.53 GLLGQYY19 pKa = 9.42 LVRR22 pKa = 11.84 WLRR25 pKa = 11.84 PYY27 pKa = 9.09 EE28 pKa = 4.33 TGTFTALPLDD38 pKa = 3.78 EE39 pKa = 4.26 QMSYY43 pKa = 10.06 IYY45 pKa = 10.5 RR46 pKa = 11.84 PFWGSTRR53 pKa = 11.84 PPALARR59 pKa = 11.84 AALSYY64 pKa = 10.73 LGADD68 pKa = 3.37 MPIQVVLSDD77 pKa = 4.16 SDD79 pKa = 3.73 FKK81 pKa = 11.39 HH82 pKa = 6.19 ILTYY86 pKa = 10.95 LPTSPDD92 pKa = 2.98 LPATVDD98 pKa = 3.35 VPAWVFTKK106 pKa = 10.57 AGTMQHH112 pKa = 6.02 FRR114 pKa = 11.84 PKK116 pKa = 10.06 AHH118 pKa = 6.82 AKK120 pKa = 10.21 AVTKK124 pKa = 10.91 ANIFLDD130 pKa = 3.71 EE131 pKa = 4.15 VLRR134 pKa = 11.84 DD135 pKa = 3.89 ARR137 pKa = 11.84 RR138 pKa = 11.84 LDD140 pKa = 3.57 ANFVMAAWPYY150 pKa = 11.22 LDD152 pKa = 5.15 DD153 pKa = 4.65 LRR155 pKa = 11.84 SNGITHH161 pKa = 6.98 DD162 pKa = 3.52 QAVAFVLYY170 pKa = 10.41 AWALKK175 pKa = 10.4 GKK177 pKa = 7.9 TVDD180 pKa = 3.37 NLRR183 pKa = 11.84 WAHH186 pKa = 5.99 FCCSQRR192 pKa = 11.84 KK193 pKa = 6.41 EE194 pKa = 4.08 AKK196 pKa = 9.53 EE197 pKa = 3.65 VSNFLKK203 pKa = 10.78 AVGGNAHH210 pKa = 7.29 PLGAMMVEE218 pKa = 4.34 TDD220 pKa = 3.41 TLAGRR225 pKa = 11.84 GTGEE229 pKa = 3.98 STLLEE234 pKa = 4.38 GAIEE238 pKa = 4.33 RR239 pKa = 11.84 CDD241 pKa = 3.61 LAALRR246 pKa = 11.84 ASKK249 pKa = 10.37 LAEE252 pKa = 3.92 FDD254 pKa = 3.87 EE255 pKa = 4.77 NSLRR259 pKa = 11.84 RR260 pKa = 11.84 AIRR263 pKa = 11.84 RR264 pKa = 11.84 ILVSEE269 pKa = 4.46 ISRR272 pKa = 11.84 NDD274 pKa = 3.09 TGGYY278 pKa = 9.78 HH279 pKa = 7.04 LAFPALSDD287 pKa = 3.13 HH288 pKa = 6.48 WASRR292 pKa = 11.84 WRR294 pKa = 11.84 WAVNGSHH301 pKa = 6.02 SAEE304 pKa = 4.08 VDD306 pKa = 3.67 RR307 pKa = 11.84 QCGFTPPPLPGGRR320 pKa = 11.84 KK321 pKa = 5.08 YY322 pKa = 10.38 HH323 pKa = 5.71 RR324 pKa = 11.84 RR325 pKa = 11.84 AWLEE329 pKa = 3.62 TRR331 pKa = 11.84 ADD333 pKa = 3.8 DD334 pKa = 5.68 PRR336 pKa = 11.84 PGWDD340 pKa = 2.71 GTTYY344 pKa = 10.87 VSASDD349 pKa = 3.53 KK350 pKa = 11.12 LEE352 pKa = 4.23 HH353 pKa = 6.47 GKK355 pKa = 8.01 TRR357 pKa = 11.84 TILACDD363 pKa = 3.42 TRR365 pKa = 11.84 SYY367 pKa = 11.33 LAFEE371 pKa = 4.57 HH372 pKa = 7.05 LMGTVEE378 pKa = 4.2 KK379 pKa = 9.57 AWRR382 pKa = 11.84 GTRR385 pKa = 11.84 VILNPGKK392 pKa = 10.44 GGHH395 pKa = 6.45 IGMANRR401 pKa = 11.84 VEE403 pKa = 4.29 RR404 pKa = 11.84 NRR406 pKa = 11.84 NRR408 pKa = 11.84 SGVSMMLDD416 pKa = 3.08 YY417 pKa = 11.58 DD418 pKa = 4.48 DD419 pKa = 5.94 FNSHH423 pKa = 6.72 HH424 pKa = 7.15 SNEE427 pKa = 3.88 AMKK430 pKa = 10.54 ILVEE434 pKa = 4.36 EE435 pKa = 4.69 TCQLTGYY442 pKa = 8.8 PAEE445 pKa = 4.32 LAAPLIASFDD455 pKa = 3.46 KK456 pKa = 10.82 QRR458 pKa = 11.84 IYY460 pKa = 11.2 VAGKK464 pKa = 8.55 YY465 pKa = 10.07 VGVSRR470 pKa = 11.84 GTLMSGHH477 pKa = 6.99 RR478 pKa = 11.84 CTTYY482 pKa = 10.57 INSVLNMAYY491 pKa = 10.86 LMVVLGDD498 pKa = 3.92 DD499 pKa = 4.11 FVMEE503 pKa = 4.64 RR504 pKa = 11.84 PTLHH508 pKa = 6.81 VGDD511 pKa = 4.79 DD512 pKa = 3.53 VFFGVRR518 pKa = 11.84 SYY520 pKa = 11.8 SEE522 pKa = 3.71 ACYY525 pKa = 10.04 VARR528 pKa = 11.84 SVLASRR534 pKa = 11.84 LRR536 pKa = 11.84 MNRR539 pKa = 11.84 SKK541 pKa = 11.04 QSVGHH546 pKa = 5.87 VATEE550 pKa = 3.89 FLRR553 pKa = 11.84 VSSRR557 pKa = 11.84 ARR559 pKa = 11.84 DD560 pKa = 3.38 SYY562 pKa = 11.81 GYY564 pKa = 9.97 LCRR567 pKa = 11.84 AISSCVSGNWVSDD580 pKa = 3.52 KK581 pKa = 11.31 LLDD584 pKa = 4.33 PYY586 pKa = 10.9 EE587 pKa = 4.73 ALNTMLGSARR597 pKa = 11.84 TLMNRR602 pKa = 11.84 AISHH606 pKa = 6.85 DD607 pKa = 4.48 LPLLLASAIKK617 pKa = 9.49 RR618 pKa = 11.84 TVRR621 pKa = 11.84 ADD623 pKa = 3.43 GLNDD627 pKa = 3.75 RR628 pKa = 11.84 LLTEE632 pKa = 4.7 LLLGSVAVNNGPQFSSSGTHH652 pKa = 6.08 RR653 pKa = 11.84 IVWVQPQSRR662 pKa = 11.84 LLDD665 pKa = 4.18 PLDD668 pKa = 3.7 VSLLPRR674 pKa = 11.84 EE675 pKa = 4.35 STASYY680 pKa = 10.56 LVNCAQPIEE689 pKa = 4.42 TQTLARR695 pKa = 11.84 VGISPVGTMLASSYY709 pKa = 11.29 AKK711 pKa = 10.38 SLDD714 pKa = 3.51 YY715 pKa = 11.31 ARR717 pKa = 11.84 TQTFRR722 pKa = 11.84 LSFSAVHH729 pKa = 6.64 TYY731 pKa = 10.41 RR732 pKa = 11.84 AVGSTTAEE740 pKa = 4.02 LALASPKK747 pKa = 10.01 PRR749 pKa = 11.84 GILNQYY755 pKa = 9.19 PLLVLVKK762 pKa = 10.3 HH763 pKa = 6.36 RR764 pKa = 11.84 LPEE767 pKa = 3.99 NVLRR771 pKa = 11.84 EE772 pKa = 4.12 VVATVGGNGHH782 pKa = 6.61 AEE784 pKa = 4.14 RR785 pKa = 11.84 IVVEE789 pKa = 3.62 AWGEE793 pKa = 3.92 YY794 pKa = 9.04 RR795 pKa = 11.84 HH796 pKa = 6.08 GCIINSVLSFSDD808 pKa = 3.88 ASALGKK814 pKa = 9.85 RR815 pKa = 11.84 VKK817 pKa = 10.83 ASVLTSGRR825 pKa = 11.84 HH826 pKa = 5.33 CYY828 pKa = 8.68 VV829 pKa = 2.93
Molecular weight: 91.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.582
IPC2_protein 8.507
IPC_protein 8.419
Toseland 8.697
ProMoST 8.858
Dawson 9.18
Bjellqvist 9.194
Wikipedia 9.355
Rodwell 9.253
Grimsley 9.136
Solomon 9.238
Lehninger 9.194
Nozaki 9.253
DTASelect 9.048
Thurlkill 9.092
EMBOSS 9.297
Sillero 9.311
Patrickios 4.52
IPC_peptide 9.224
IPC2_peptide 8.141
IPC2.peptide.svr19 7.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1586
757
829
793.0
85.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.98 ± 1.26
1.387 ± 0.116
5.422 ± 0.004
4.224 ± 0.002
3.342 ± 0.286
8.071 ± 0.611
3.153 ± 0.01
3.153 ± 0.067
2.774 ± 0.386
9.079 ± 1.138
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.702 ± 0.455
3.468 ± 0.097
5.99 ± 1.055
1.892 ± 0.102
7.251 ± 0.687
7.755 ± 0.209
6.81 ± 0.498
7.818 ± 0.014
1.513 ± 0.19
3.216 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here