Lactococcus phage CHPC781

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus CHPC781

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650ESZ1|A0A650ESZ1_9CAUD Uncharacterized protein OS=Lactococcus phage CHPC781 OX=2675252 GN=CHPC781_000755 PE=4 SV=1
MM1 pKa = 7.56EE2 pKa = 5.5FDD4 pKa = 6.13SYY6 pKa = 11.42IDD8 pKa = 3.32WYY10 pKa = 11.58NNLLTMPLNDD20 pKa = 3.86VILGVKK26 pKa = 8.96DD27 pKa = 3.57TIEE30 pKa = 4.55DD31 pKa = 3.52KK32 pKa = 10.66TVYY35 pKa = 10.62LSLSDD40 pKa = 3.95SKK42 pKa = 10.99VLKK45 pKa = 9.75MDD47 pKa = 3.09NTSFVMGYY55 pKa = 9.13YY56 pKa = 8.74YY57 pKa = 10.45QVVLSVKK64 pKa = 10.52DD65 pKa = 3.51VDD67 pKa = 4.41DD68 pKa = 4.44EE69 pKa = 4.52LVGLVGDD76 pKa = 4.15VLQNGWNMTNWSEE89 pKa = 4.32NSHH92 pKa = 6.58LYY94 pKa = 10.7NYY96 pKa = 8.61TGTVYY101 pKa = 10.42LPCGAGGQAWQQ112 pKa = 3.59

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650EUA1|A0A650EUA1_9CAUD Holliday junction endonuclease OS=Lactococcus phage CHPC781 OX=2675252 GN=CHPC781_000776 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.54SIVNAQHH21 pKa = 6.05NKK23 pKa = 9.46KK24 pKa = 10.06KK25 pKa = 10.41ADD27 pKa = 4.14KK28 pKa = 10.4IPVRR32 pKa = 11.84TYY34 pKa = 8.89YY35 pKa = 10.71CKK37 pKa = 9.99WCNLYY42 pKa = 10.42HH43 pKa = 7.32LSSQQRR49 pKa = 11.84LNIKK53 pKa = 9.21TGVIGG58 pKa = 3.97

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

8578

30

996

153.2

17.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.957 ± 0.551

0.804 ± 0.209

5.642 ± 0.259

7.426 ± 0.67

4.465 ± 0.338

6.388 ± 0.651

1.376 ± 0.215

7.111 ± 0.245

9.21 ± 0.494

8.662 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.156

6.61 ± 0.258

2.098 ± 0.235

3.497 ± 0.286

3.591 ± 0.312

6.074 ± 0.474

6.342 ± 0.377

6.54 ± 0.455

1.387 ± 0.187

4.325 ± 0.42
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski