Amino acid dipepetide frequency for Mycoplasma sp. CAG:877

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.546AlaAla: 2.546 ± 0.103	0.574AlaCys: 0.574 ± 0.034	2.419AlaAsp: 2.419 ± 0.084	2.481AlaGlu: 2.481 ± 0.083	2.034AlaPhe: 2.034 ± 0.071	3.007AlaGly: 3.007 ± 0.095	0.691AlaHis: 0.691 ± 0.041	4.699AlaIle: 4.699 ± 0.114	4.01AlaLys: 4.01 ± 0.106	4.46AlaLeu: 4.46 ± 0.123	1.182AlaMet: 1.182 ± 0.053	2.79AlaAsn: 2.79 ± 0.092	1.089AlaPro: 1.089 ± 0.061	0.964AlaGln: 0.964 ± 0.06	1.842AlaArg: 1.842 ± 0.071	3.294AlaSer: 3.294 ± 0.097	2.983AlaThr: 2.983 ± 0.074	2.809AlaVal: 2.809 ± 0.097	0.266AlaTrp: 0.266 ± 0.027	2.129AlaTyr: 2.129 ± 0.072	0.0AlaXaa: 0.0 ± 0.0
Cys	0.452CysAla: 0.452 ± 0.034	0.201CysCys: 0.201 ± 0.024	0.696CysAsp: 0.696 ± 0.046	0.586CysGlu: 0.586 ± 0.039	0.543CysPhe: 0.543 ± 0.038	0.806CysGly: 0.806 ± 0.046	0.182CysHis: 0.182 ± 0.023	0.885CysIle: 0.885 ± 0.054	0.849CysLys: 0.849 ± 0.045	1.053CysLeu: 1.053 ± 0.049	0.246CysMet: 0.246 ± 0.023	0.672CysAsn: 0.672 ± 0.041	0.431CysPro: 0.431 ± 0.033	0.289CysGln: 0.289 ± 0.028	0.443CysArg: 0.443 ± 0.036	0.876CysSer: 0.876 ± 0.054	0.61CysThr: 0.61 ± 0.04	0.596CysVal: 0.596 ± 0.038	0.1CysTrp: 0.1 ± 0.016	0.572CysTyr: 0.572 ± 0.043	0.0CysXaa: 0.0 ± 0.0
Asp	2.701AspAla: 2.701 ± 0.082	0.62AspCys: 0.62 ± 0.043	3.713AspAsp: 3.713 ± 0.127	4.864AspGlu: 4.864 ± 0.121	2.986AspPhe: 2.986 ± 0.077	3.61AspGly: 3.61 ± 0.11	0.569AspHis: 0.569 ± 0.035	5.84AspIle: 5.84 ± 0.109	6.151AspLys: 6.151 ± 0.127	5.618AspLeu: 5.618 ± 0.121	1.467AspMet: 1.467 ± 0.059	4.218AspAsn: 4.218 ± 0.123	1.388AspPro: 1.388 ± 0.065	1.0AspGln: 1.0 ± 0.051	1.878AspArg: 1.878 ± 0.077	3.974AspSer: 3.974 ± 0.113	3.208AspThr: 3.208 ± 0.092	3.936AspVal: 3.936 ± 0.116	0.349AspTrp: 0.349 ± 0.03	3.737AspTyr: 3.737 ± 0.1	0.0AspXaa: 0.0 ± 0.0
Glu	3.402GluAla: 3.402 ± 0.108	0.663GluCys: 0.663 ± 0.034	4.122GluAsp: 4.122 ± 0.111	7.237GluGlu: 7.237 ± 0.203	2.981GluPhe: 2.981 ± 0.087	2.96GluGly: 2.96 ± 0.095	0.871GluHis: 0.871 ± 0.048	6.457GluIle: 6.457 ± 0.146	8.46GluLys: 8.46 ± 0.189	6.778GluLeu: 6.778 ± 0.153	1.907GluMet: 1.907 ± 0.066	5.005GluAsn: 5.005 ± 0.121	1.318GluPro: 1.318 ± 0.062	1.931GluGln: 1.931 ± 0.083	2.591GluArg: 2.591 ± 0.107	3.261GluSer: 3.261 ± 0.074	3.515GluThr: 3.515 ± 0.099	5.33GluVal: 5.33 ± 0.112	0.409GluTrp: 0.409 ± 0.035	3.419GluTyr: 3.419 ± 0.096	0.007GluXaa: 0.007 ± 0.004
Phe	2.048PheAla: 2.048 ± 0.075	0.526PheCys: 0.526 ± 0.033	2.876PheAsp: 2.876 ± 0.084	2.562PheGlu: 2.562 ± 0.076	1.852PhePhe: 1.852 ± 0.075	2.591PheGly: 2.591 ± 0.083	0.495PheHis: 0.495 ± 0.034	4.254PheIle: 4.254 ± 0.119	3.598PheLys: 3.598 ± 0.094	4.153PheLeu: 4.153 ± 0.128	0.959PheMet: 0.959 ± 0.051	2.924PheAsn: 2.924 ± 0.088	1.199PhePro: 1.199 ± 0.051	0.84PheGln: 0.84 ± 0.044	1.278PheArg: 1.278 ± 0.06	3.338PheSer: 3.338 ± 0.114	2.589PheThr: 2.589 ± 0.09	2.73PheVal: 2.73 ± 0.092	0.239PheTrp: 0.239 ± 0.023	2.23PheTyr: 2.23 ± 0.082	0.0PheXaa: 0.0 ± 0.0
Gly	2.627GlyAla: 2.627 ± 0.09	0.816GlyCys: 0.816 ± 0.044	3.036GlyAsp: 3.036 ± 0.092	3.481GlyGlu: 3.481 ± 0.099	2.613GlyPhe: 2.613 ± 0.073	3.263GlyGly: 3.263 ± 0.162	0.88GlyHis: 0.88 ± 0.049	5.127GlyIle: 5.127 ± 0.116	5.007GlyLys: 5.007 ± 0.112	4.632GlyLeu: 4.632 ± 0.102	1.414GlyMet: 1.414 ± 0.059	3.383GlyAsn: 3.383 ± 0.153	0.998GlyPro: 0.998 ± 0.051	1.177GlyGln: 1.177 ± 0.058	1.828GlyArg: 1.828 ± 0.069	3.572GlySer: 3.572 ± 0.124	3.725GlyThr: 3.725 ± 0.13	4.113GlyVal: 4.113 ± 0.112	0.306GlyTrp: 0.306 ± 0.032	3.213GlyTyr: 3.213 ± 0.09	0.0GlyXaa: 0.0 ± 0.0
His	0.534HisAla: 0.534 ± 0.039	0.16HisCys: 0.16 ± 0.021	0.665HisAsp: 0.665 ± 0.037	0.859HisGlu: 0.859 ± 0.042	0.605HisPhe: 0.605 ± 0.035	0.823HisGly: 0.823 ± 0.045	0.287HisHis: 0.287 ± 0.029	1.144HisIle: 1.144 ± 0.047	0.988HisLys: 0.988 ± 0.045	1.069HisLeu: 1.069 ± 0.053	0.242HisMet: 0.242 ± 0.024	0.845HisAsn: 0.845 ± 0.046	0.61HisPro: 0.61 ± 0.04	0.313HisGln: 0.313 ± 0.028	0.495HisArg: 0.495 ± 0.038	0.766HisSer: 0.766 ± 0.049	0.727HisThr: 0.727 ± 0.041	0.773HisVal: 0.773 ± 0.048	0.067HisTrp: 0.067 ± 0.012	0.732HisTyr: 0.732 ± 0.042	0.0HisXaa: 0.0 ± 0.0
Ile	4.417IleAla: 4.417 ± 0.116	1.079IleCys: 1.079 ± 0.063	6.292IleAsp: 6.292 ± 0.125	6.23IleGlu: 6.23 ± 0.144	3.979IlePhe: 3.979 ± 0.123	4.876IleGly: 4.876 ± 0.132	1.048IleHis: 1.048 ± 0.057	9.12IleIle: 9.12 ± 0.226	8.41IleLys: 8.41 ± 0.167	8.438IleLeu: 8.438 ± 0.183	1.952IleMet: 1.952 ± 0.069	6.12IleAsn: 6.12 ± 0.119	3.026IlePro: 3.026 ± 0.093	1.584IleGln: 1.584 ± 0.066	2.931IleArg: 2.931 ± 0.097	6.687IleSer: 6.687 ± 0.139	5.637IleThr: 5.637 ± 0.133	6.474IleVal: 6.474 ± 0.156	0.445IleTrp: 0.445 ± 0.038	4.146IleTyr: 4.146 ± 0.112	0.0IleXaa: 0.0 ± 0.0
Lys	3.981LysAla: 3.981 ± 0.116	0.876LysCys: 0.876 ± 0.049	6.543LysAsp: 6.543 ± 0.13	9.134LysGlu: 9.134 ± 0.221	3.156LysPhe: 3.156 ± 0.086	3.962LysGly: 3.962 ± 0.087	1.048LysHis: 1.048 ± 0.051	8.481LysIle: 8.481 ± 0.169	9.862LysLys: 9.862 ± 0.192	7.596LysLeu: 7.596 ± 0.166	2.426LysMet: 2.426 ± 0.071	7.144LysAsn: 7.144 ± 0.136	1.747LysPro: 1.747 ± 0.079	2.261LysGln: 2.261 ± 0.082	3.474LysArg: 3.474 ± 0.087	5.017LysSer: 5.017 ± 0.107	5.07LysThr: 5.07 ± 0.113	6.307LysVal: 6.307 ± 0.123	0.471LysTrp: 0.471 ± 0.033	5.316LysTyr: 5.316 ± 0.126	0.0LysXaa: 0.0 ± 0.0
Leu	4.665LeuAla: 4.665 ± 0.119	0.943LeuCys: 0.943 ± 0.053	5.594LeuAsp: 5.594 ± 0.134	7.075LeuGlu: 7.075 ± 0.188	4.201LeuPhe: 4.201 ± 0.127	5.22LeuGly: 5.22 ± 0.124	1.072LeuHis: 1.072 ± 0.05	7.91LeuIle: 7.91 ± 0.188	8.23LeuLys: 8.23 ± 0.153	8.508LeuLeu: 8.508 ± 0.198	1.928LeuMet: 1.928 ± 0.071	5.701LeuAsn: 5.701 ± 0.133	2.625LeuPro: 2.625 ± 0.078	2.079LeuGln: 2.079 ± 0.081	2.945LeuArg: 2.945 ± 0.084	6.34LeuSer: 6.34 ± 0.145	5.326LeuThr: 5.326 ± 0.119	6.211LeuVal: 6.211 ± 0.115	0.392LeuTrp: 0.392 ± 0.029	3.814LeuTyr: 3.814 ± 0.105	0.002LeuXaa: 0.002 ± 0.002
Met	1.28MetAla: 1.28 ± 0.059	0.167MetCys: 0.167 ± 0.018	1.414MetAsp: 1.414 ± 0.058	1.546MetGlu: 1.546 ± 0.056	1.046MetPhe: 1.046 ± 0.051	1.184MetGly: 1.184 ± 0.056	0.28MetHis: 0.28 ± 0.031	1.993MetIle: 1.993 ± 0.071	2.428MetLys: 2.428 ± 0.071	2.053MetLeu: 2.053 ± 0.071	0.61MetMet: 0.61 ± 0.038	1.534MetAsn: 1.534 ± 0.068	0.749MetPro: 0.749 ± 0.045	0.553MetGln: 0.553 ± 0.041	0.775MetArg: 0.775 ± 0.043	1.529MetSer: 1.529 ± 0.057	1.201MetThr: 1.201 ± 0.05	1.421MetVal: 1.421 ± 0.061	0.072MetTrp: 0.072 ± 0.012	1.007MetTyr: 1.007 ± 0.056	0.0MetXaa: 0.0 ± 0.0
Asn	2.641AsnAla: 2.641 ± 0.073	0.746AsnCys: 0.746 ± 0.05	4.187AsnAsp: 4.187 ± 0.109	4.275AsnGlu: 4.275 ± 0.1	2.742AsnPhe: 2.742 ± 0.091	4.005AsnGly: 4.005 ± 0.126	0.809AsnHis: 0.809 ± 0.04	6.637AsnIle: 6.637 ± 0.149	6.677AsnLys: 6.677 ± 0.159	5.67AsnLeu: 5.67 ± 0.122	1.598AsnMet: 1.598 ± 0.072	5.816AsnAsn: 5.816 ± 0.169	2.05AsnPro: 2.05 ± 0.062	1.541AsnGln: 1.541 ± 0.076	1.969AsnArg: 1.969 ± 0.072	4.424AsnSer: 4.424 ± 0.122	3.835AsnThr: 3.835 ± 0.103	4.074AsnVal: 4.074 ± 0.118	0.371AsnTrp: 0.371 ± 0.029	3.962AsnTyr: 3.962 ± 0.133	0.0AsnXaa: 0.0 ± 0.0
Pro	1.139ProAla: 1.139 ± 0.052	0.263ProCys: 0.263 ± 0.026	1.591ProAsp: 1.591 ± 0.064	1.983ProGlu: 1.983 ± 0.072	1.23ProPhe: 1.23 ± 0.053	1.347ProGly: 1.347 ± 0.065	0.421ProHis: 0.421 ± 0.033	2.562ProIle: 2.562 ± 0.082	2.101ProLys: 2.101 ± 0.062	2.026ProLeu: 2.026 ± 0.075	0.49ProMet: 0.49 ± 0.029	1.756ProAsn: 1.756 ± 0.065	0.431ProPro: 0.431 ± 0.032	0.567ProGln: 0.567 ± 0.045	0.811ProArg: 0.811 ± 0.042	1.754ProSer: 1.754 ± 0.078	1.816ProThr: 1.816 ± 0.067	2.074ProVal: 2.074 ± 0.088	0.211ProTrp: 0.211 ± 0.02	1.371ProTyr: 1.371 ± 0.058	0.0ProXaa: 0.0 ± 0.0
Gln	1.323GlnAla: 1.323 ± 0.069	0.163GlnCys: 0.163 ± 0.021	1.246GlnAsp: 1.246 ± 0.054	1.912GlnGlu: 1.912 ± 0.085	0.864GlnPhe: 0.864 ± 0.047	1.084GlnGly: 1.084 ± 0.054	0.263GlnHis: 0.263 ± 0.026	2.046GlnIle: 2.046 ± 0.072	2.213GlnLys: 2.213 ± 0.076	1.938GlnLeu: 1.938 ± 0.069	0.548GlnMet: 0.548 ± 0.036	1.457GlnAsn: 1.457 ± 0.071	0.55GlnPro: 0.55 ± 0.05	0.675GlnGln: 0.675 ± 0.055	0.734GlnArg: 0.734 ± 0.045	1.151GlnSer: 1.151 ± 0.058	1.215GlnThr: 1.215 ± 0.062	1.622GlnVal: 1.622 ± 0.066	0.11GlnTrp: 0.11 ± 0.017	0.983GlnTyr: 0.983 ± 0.051	0.002GlnXaa: 0.002 ± 0.003
Arg	1.534ArgAla: 1.534 ± 0.058	0.376ArgCys: 0.376 ± 0.031	2.16ArgAsp: 2.16 ± 0.08	3.103ArgGlu: 3.103 ± 0.087	1.256ArgPhe: 1.256 ± 0.053	1.684ArgGly: 1.684 ± 0.069	0.548ArgHis: 0.548 ± 0.036	2.854ArgIle: 2.854 ± 0.093	3.244ArgLys: 3.244 ± 0.091	2.971ArgLeu: 2.971 ± 0.09	0.916ArgMet: 0.916 ± 0.051	2.089ArgAsn: 2.089 ± 0.073	0.833ArgPro: 0.833 ± 0.045	0.84ArgGln: 0.84 ± 0.058	1.491ArgArg: 1.491 ± 0.066	1.639ArgSer: 1.639 ± 0.062	1.668ArgThr: 1.668 ± 0.054	2.519ArgVal: 2.519 ± 0.091	0.184ArgTrp: 0.184 ± 0.022	1.603ArgTyr: 1.603 ± 0.064	0.0ArgXaa: 0.0 ± 0.0
Ser	2.668SerAla: 2.668 ± 0.077	0.723SerCys: 0.723 ± 0.05	4.058SerAsp: 4.058 ± 0.112	3.955SerGlu: 3.955 ± 0.107	3.141SerPhe: 3.141 ± 0.092	3.857SerGly: 3.857 ± 0.124	0.828SerHis: 0.828 ± 0.052	6.077SerIle: 6.077 ± 0.148	5.976SerLys: 5.976 ± 0.132	6.4SerLeu: 6.4 ± 0.14	1.347SerMet: 1.347 ± 0.061	4.651SerAsn: 4.651 ± 0.136	1.502SerPro: 1.502 ± 0.059	1.392SerGln: 1.392 ± 0.06	2.302SerArg: 2.302 ± 0.07	5.417SerSer: 5.417 ± 0.171	3.902SerThr: 3.902 ± 0.139	3.861SerVal: 3.861 ± 0.112	0.476SerTrp: 0.476 ± 0.041	3.33SerTyr: 3.33 ± 0.118	0.005SerXaa: 0.005 ± 0.003
Thr	2.555ThrAla: 2.555 ± 0.089	0.751ThrCys: 0.751 ± 0.049	3.457ThrAsp: 3.457 ± 0.112	3.282ThrGlu: 3.282 ± 0.092	2.402ThrPhe: 2.402 ± 0.069	3.888ThrGly: 3.888 ± 0.103	0.768ThrHis: 0.768 ± 0.044	5.826ThrIle: 5.826 ± 0.157	5.125ThrLys: 5.125 ± 0.113	5.208ThrLeu: 5.208 ± 0.121	1.136ThrMet: 1.136 ± 0.053	3.945ThrAsn: 3.945 ± 0.122	1.945ThrPro: 1.945 ± 0.072	1.06ThrGln: 1.06 ± 0.056	1.639ThrArg: 1.639 ± 0.062	4.163ThrSer: 4.163 ± 0.123	3.986ThrThr: 3.986 ± 0.136	3.656ThrVal: 3.656 ± 0.13	0.366ThrTrp: 0.366 ± 0.035	2.914ThrTyr: 2.914 ± 0.104	0.0ThrXaa: 0.0 ± 0.0
Val	3.416ValAla: 3.416 ± 0.104	0.813ValCys: 0.813 ± 0.047	4.149ValAsp: 4.149 ± 0.126	4.524ValGlu: 4.524 ± 0.122	3.015ValPhe: 3.015 ± 0.097	3.888ValGly: 3.888 ± 0.104	0.787ValHis: 0.787 ± 0.038	6.225ValIle: 6.225 ± 0.123	5.582ValLys: 5.582 ± 0.127	6.517ValLeu: 6.517 ± 0.131	1.392ValMet: 1.392 ± 0.058	3.905ValAsn: 3.905 ± 0.099	1.969ValPro: 1.969 ± 0.067	1.33ValGln: 1.33 ± 0.062	2.093ValArg: 2.093 ± 0.07	4.976ValSer: 4.976 ± 0.131	3.864ValThr: 3.864 ± 0.117	5.019ValVal: 5.019 ± 0.126	0.376ValTrp: 0.376 ± 0.03	2.921ValTyr: 2.921 ± 0.114	0.0ValXaa: 0.0 ± 0.0
Trp	0.285TrpAla: 0.285 ± 0.028	0.112TrpCys: 0.112 ± 0.017	0.287TrpAsp: 0.287 ± 0.026	0.354TrpGlu: 0.354 ± 0.026	0.282TrpPhe: 0.282 ± 0.027	0.242TrpGly: 0.242 ± 0.025	0.091TrpHis: 0.091 ± 0.016	0.433TrpIle: 0.433 ± 0.032	0.488TrpLys: 0.488 ± 0.04	0.545TrpLeu: 0.545 ± 0.037	0.136TrpMet: 0.136 ± 0.017	0.431TrpAsn: 0.431 ± 0.033	0.12TrpPro: 0.12 ± 0.015	0.144TrpGln: 0.144 ± 0.019	0.191TrpArg: 0.191 ± 0.021	0.325TrpSer: 0.325 ± 0.03	0.316TrpThr: 0.316 ± 0.03	0.285TrpVal: 0.285 ± 0.028	0.065TrpTrp: 0.065 ± 0.012	0.395TrpTyr: 0.395 ± 0.038	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.053TyrAla: 2.053 ± 0.065	0.517TyrCys: 0.517 ± 0.039	3.321TyrAsp: 3.321 ± 0.094	3.175TyrGlu: 3.175 ± 0.101	2.352TyrPhe: 2.352 ± 0.082	2.914TyrGly: 2.914 ± 0.101	0.766TyrHis: 0.766 ± 0.042	4.321TyrIle: 4.321 ± 0.115	4.467TyrLys: 4.467 ± 0.126	5.146TyrLeu: 5.146 ± 0.149	0.94TyrMet: 0.94 ± 0.049	3.646TyrAsn: 3.646 ± 0.102	1.366TyrPro: 1.366 ± 0.054	1.56TyrGln: 1.56 ± 0.076	1.802TyrArg: 1.802 ± 0.077	3.366TyrSer: 3.366 ± 0.1	2.881TyrThr: 2.881 ± 0.119	2.952TyrVal: 2.952 ± 0.096	0.251TyrTrp: 0.251 ± 0.024	2.964TyrTyr: 2.964 ± 0.112	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.005XaaGlu: 0.005 ± 0.003	0.002XaaPhe: 0.002 ± 0.002	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.007XaaLys: 0.007 ± 0.004	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.003	0.019XaaXaa: 0.019 ± 0.009

Statistics based on 1407 proteins (417977 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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