Croceitalea dokdonensis DOKDO 023
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3682 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P7AD04|A0A0P7AD04_9FLAO 3'-5' exonuclease OS=Croceitalea dokdonensis DOKDO 023 OX=1300341 GN=I595_3051 PE=4 SV=1
MM1 pKa = 7.74 LMPMVLRR8 pKa = 11.84 FFAVISVFLLLSCGGDD24 pKa = 3.7 DD25 pKa = 4.12 EE26 pKa = 5.72 PEE28 pKa = 4.05 NQMTTSVTVAFLAQEE43 pKa = 4.44 TTGSEE48 pKa = 3.88 ALGNNLPSVSVRR60 pKa = 11.84 GTVASEE66 pKa = 4.07 VSVSVLLTNTGTATLGEE83 pKa = 4.69 DD84 pKa = 3.98 FSFASPTVISIPIGNYY100 pKa = 10.23 DD101 pKa = 3.6 GTALTAIPLPGLSILDD117 pKa = 3.66 DD118 pKa = 3.78 TVQEE122 pKa = 4.21 GFEE125 pKa = 4.3 TIIFSLDD132 pKa = 3.26 NPTEE136 pKa = 4.2 GVNLGDD142 pKa = 3.91 VATTTYY148 pKa = 10.02 TINDD152 pKa = 4.14 DD153 pKa = 3.46 EE154 pKa = 4.59 TVQVGFSQAMASDD167 pKa = 4.1 RR168 pKa = 11.84 EE169 pKa = 4.39 NVGGNLPTLFVTGTLSEE186 pKa = 4.88 DD187 pKa = 3.11 KK188 pKa = 10.49 TITLTAVDD196 pKa = 4.59 SGDD199 pKa = 3.3 ATLGQDD205 pKa = 3.7 YY206 pKa = 10.73 NVASPLVVTIPAGTYY221 pKa = 10.54 DD222 pKa = 3.78 GTTATALTIPALAIIDD238 pKa = 3.75 DD239 pKa = 4.3 TVPEE243 pKa = 4.04 EE244 pKa = 4.38 SEE246 pKa = 4.48 SFEE249 pKa = 4.41 LTLSDD254 pKa = 4.3 PSNGLLLNEE263 pKa = 5.53 LDD265 pKa = 3.11 RR266 pKa = 11.84 TMYY269 pKa = 9.87 TIEE272 pKa = 4.28 GVAPCEE278 pKa = 3.83 NGRR281 pKa = 11.84 ANGFPCNGFDD291 pKa = 3.51 LLARR295 pKa = 11.84 MEE297 pKa = 4.56 HH298 pKa = 5.88 STFSSTQGNDD308 pKa = 2.03 IWGWTDD314 pKa = 2.76 TATGHH319 pKa = 6.31 EE320 pKa = 4.43 YY321 pKa = 11.41 ALVALNNGTAFVDD334 pKa = 3.75 ITADD338 pKa = 3.53 DD339 pKa = 3.88 PVYY342 pKa = 10.65 LGKK345 pKa = 10.66 LPSATGSSVWRR356 pKa = 11.84 DD357 pKa = 3.0 VKK359 pKa = 10.8 IYY361 pKa = 10.63 GNHH364 pKa = 6.38 AYY366 pKa = 9.27 IVSEE370 pKa = 4.01 ASGHH374 pKa = 4.89 GMQVFDD380 pKa = 4.03 LTRR383 pKa = 11.84 LRR385 pKa = 11.84 NVANPPTDD393 pKa = 3.66 FTQDD397 pKa = 2.62 ARR399 pKa = 11.84 YY400 pKa = 7.96 TDD402 pKa = 3.22 IGNAHH407 pKa = 5.85 NVVINEE413 pKa = 4.15 QNGFAYY419 pKa = 9.56 PVGTARR425 pKa = 11.84 NDD427 pKa = 3.55 TFNGGVHH434 pKa = 6.47 FVDD437 pKa = 3.65 IQNPTSPTAAGGYY450 pKa = 9.4 GDD452 pKa = 4.67 NGYY455 pKa = 7.73 THH457 pKa = 7.89 DD458 pKa = 4.1 AQVVNYY464 pKa = 7.53 EE465 pKa = 4.58 GPDD468 pKa = 3.02 ADD470 pKa = 3.79 YY471 pKa = 10.26 TGRR474 pKa = 11.84 EE475 pKa = 3.78 IFIGANEE482 pKa = 4.01 DD483 pKa = 3.19 QIAIVDD489 pKa = 3.81 ITDD492 pKa = 3.47 KK493 pKa = 11.48 ANPTEE498 pKa = 4.22 IATITYY504 pKa = 9.87 GNLGYY509 pKa = 7.89 THH511 pKa = 6.47 QGWFTEE517 pKa = 4.08 DD518 pKa = 2.99 QRR520 pKa = 11.84 FFLLGDD526 pKa = 3.76 EE527 pKa = 4.73 LDD529 pKa = 4.53 EE530 pKa = 5.86 IDD532 pKa = 4.86 FGFNSRR538 pKa = 11.84 TLVFDD543 pKa = 5.01 LQDD546 pKa = 3.54 LDD548 pKa = 4.28 NPVLHH553 pKa = 5.73 TTYY556 pKa = 10.66 LGPTAAIDD564 pKa = 3.48 HH565 pKa = 6.55 NGYY568 pKa = 9.22 VHH570 pKa = 7.32 GDD572 pKa = 3.37 EE573 pKa = 5.46 FFLANYY579 pKa = 6.66 TAGVRR584 pKa = 11.84 VLDD587 pKa = 4.27 LSDD590 pKa = 3.65 IEE592 pKa = 4.76 GGNLTEE598 pKa = 5.23 IGFFDD603 pKa = 4.21 TFPDD607 pKa = 3.33 NDD609 pKa = 3.37 VASFNGVWSVYY620 pKa = 10.4 PYY622 pKa = 10.59 FEE624 pKa = 4.12 SGKK627 pKa = 10.47 IIVNDD632 pKa = 3.37 SDD634 pKa = 3.46 TGFFIIQKK642 pKa = 8.69 SQQ644 pKa = 2.6
Molecular weight: 69.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.757
IPC_protein 3.795
Toseland 3.567
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.884
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.719
Sillero 3.91
Patrickios 1.036
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A0P7B2B5|A0A0P7B2B5_9FLAO Uncharacterized protein OS=Croceitalea dokdonensis DOKDO 023 OX=1300341 GN=I595_1994 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.27 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.04 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.3 KK41 pKa = 9.83 LTVSSEE47 pKa = 3.9 PRR49 pKa = 11.84 HH50 pKa = 5.77 KK51 pKa = 10.61 KK52 pKa = 9.84
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3682
0
3682
1160232
37
4185
315.1
35.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.12 ± 0.039
0.753 ± 0.014
5.604 ± 0.047
6.296 ± 0.039
5.225 ± 0.038
6.921 ± 0.041
1.897 ± 0.024
6.952 ± 0.033
7.094 ± 0.069
9.673 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.338 ± 0.022
5.572 ± 0.052
3.686 ± 0.028
3.744 ± 0.023
3.854 ± 0.03
5.933 ± 0.033
5.898 ± 0.057
6.446 ± 0.031
1.143 ± 0.016
3.85 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here