Streptomyces glauciniger

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239H820|A0A239H820_9ACTN Sensor histidine kinase regulating citrate/malate metabolism OS=Streptomyces glauciniger OX=235986 GN=SAMN05216252_108234 PE=4 SV=1
MM1 pKa = 7.22SHH3 pKa = 7.33DD4 pKa = 4.5PEE6 pKa = 5.74NPTPRR11 pKa = 11.84DD12 pKa = 3.36AASEE16 pKa = 4.19NPAAAAPSSSEE27 pKa = 3.86APADD31 pKa = 3.7ARR33 pKa = 11.84PTPDD37 pKa = 3.63FEE39 pKa = 5.51LPAPAPADD47 pKa = 3.71AVPADD52 pKa = 4.22AGSGADD58 pKa = 3.39SAGDD62 pKa = 3.68AGPGDD67 pKa = 4.07GAQADD72 pKa = 4.13VTAADD77 pKa = 4.38APAAPAGDD85 pKa = 4.63EE86 pKa = 4.13DD87 pKa = 4.42TKK89 pKa = 11.34VPGTVAPEE97 pKa = 3.96DD98 pKa = 3.36AAAPADD104 pKa = 4.01APGGAAALGDD114 pKa = 4.28APPVPTAHH122 pKa = 7.67PAPAPPPAGVPGAPDD137 pKa = 3.49PWAPPVPVAPGYY149 pKa = 8.28GQPGAHH155 pKa = 6.72AGYY158 pKa = 10.14NPWAPPIPQPPQEE171 pKa = 4.16QVTLTKK177 pKa = 10.51RR178 pKa = 11.84PVPEE182 pKa = 4.59DD183 pKa = 3.3AQAPGPAQQPRR194 pKa = 11.84DD195 pKa = 3.57PWAPPAPDD203 pKa = 3.42AAVTLPPQPPHH214 pKa = 5.88ATFPGYY220 pKa = 9.37PGPPGPPGYY229 pKa = 8.82PAYY232 pKa = 8.6PAHH235 pKa = 7.07PGYY238 pKa = 9.53PGYY241 pKa = 9.56PGYY244 pKa = 9.55PGQPGYY250 pKa = 9.77PGFGMGPYY258 pKa = 9.89GGWAPPVARR267 pKa = 11.84NGVGLTAMILGIVGAVLAISCFGAFLGLPLGIAAIACGIVGLRR310 pKa = 11.84IARR313 pKa = 11.84RR314 pKa = 11.84GEE316 pKa = 3.87ATNRR320 pKa = 11.84PQALTGLILGIISVVLAGGMIALVVGGIDD349 pKa = 3.49EE350 pKa = 4.56GWFDD354 pKa = 4.87GPGGDD359 pKa = 5.38DD360 pKa = 4.29LVTSRR365 pKa = 11.84NADD368 pKa = 2.94WEE370 pKa = 4.68TPLDD374 pKa = 3.93AEE376 pKa = 4.63GTALYY381 pKa = 10.57DD382 pKa = 4.16DD383 pKa = 5.01GVHH386 pKa = 5.19VTLSDD391 pKa = 3.73VQRR394 pKa = 11.84TTALPNSMLEE404 pKa = 4.05GGTAVTFSIEE414 pKa = 4.23LEE416 pKa = 4.16NTGDD420 pKa = 3.6DD421 pKa = 3.69TADD424 pKa = 3.45LSEE427 pKa = 4.89SEE429 pKa = 3.8ITAYY433 pKa = 10.55GDD435 pKa = 3.55DD436 pKa = 5.17FEE438 pKa = 7.37DD439 pKa = 3.39EE440 pKa = 4.35TLRR443 pKa = 11.84DD444 pKa = 3.46LTTGVGLPDD453 pKa = 3.35EE454 pKa = 4.63LAPGEE459 pKa = 4.24RR460 pKa = 11.84ATRR463 pKa = 11.84DD464 pKa = 2.96ITVVVPEE471 pKa = 4.41GDD473 pKa = 3.22EE474 pKa = 5.04DD475 pKa = 3.96GTFQVEE481 pKa = 4.31VAPGYY486 pKa = 10.96AYY488 pKa = 10.21DD489 pKa = 3.68YY490 pKa = 9.84TYY492 pKa = 11.2WNLDD496 pKa = 3.33VPP498 pKa = 4.46

Molecular weight: 49.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239IEG9|A0A239IEG9_9ACTN Predicted oxidoreductase OS=Streptomyces glauciniger OX=235986 GN=SAMN05216252_110124 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.88GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8673

0

8673

2848599

24

7714

328.4

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.003 ± 0.041

0.776 ± 0.008

5.982 ± 0.022

5.553 ± 0.027

2.703 ± 0.015

9.733 ± 0.031

2.334 ± 0.013

2.958 ± 0.016

1.887 ± 0.021

10.322 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.703 ± 0.011

1.681 ± 0.014

6.203 ± 0.027

2.62 ± 0.015

8.273 ± 0.029

4.966 ± 0.021

6.053 ± 0.027

8.655 ± 0.028

1.546 ± 0.012

2.05 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski