Rubus canadensis virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4MTI1|K4MTI1_9VIRU Helicase OS=Rubus canadensis virus 1 OX=1243178 PE=3 SV=1
MM1 pKa = 7.19LQINLVVVVLVSVFVVLVLTIIDD24 pKa = 3.5KK25 pKa = 10.56FEE27 pKa = 4.3RR28 pKa = 11.84EE29 pKa = 4.37NPCFIQITGEE39 pKa = 4.11SVVIKK44 pKa = 10.81GCLFDD49 pKa = 4.88KK50 pKa = 11.1DD51 pKa = 4.34FIEE54 pKa = 4.45LVKK57 pKa = 10.59GLKK60 pKa = 9.63PFHH63 pKa = 6.96HH64 pKa = 6.74EE65 pKa = 4.01LGG67 pKa = 3.82

Molecular weight: 7.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4N435|K4N435_9VIRU Movement protein TGB2 OS=Rubus canadensis virus 1 OX=1243178 PE=3 SV=1
MM1 pKa = 7.93PLTPPKK7 pKa = 10.13DD8 pKa = 3.66YY9 pKa = 10.41TGAAISVVIGLCIAFAFHH27 pKa = 7.19SLTRR31 pKa = 11.84SNLPHH36 pKa = 6.98AGDD39 pKa = 4.67NIHH42 pKa = 6.71HH43 pKa = 6.87LPHH46 pKa = 6.38GGFYY50 pKa = 10.51KK51 pKa = 10.85DD52 pKa = 3.27GTKK55 pKa = 10.2VVAYY59 pKa = 9.13GGPQSRR65 pKa = 11.84FPSSNLFSSSFSSLSVLCVILLLSGLIYY93 pKa = 10.71ASNKK97 pKa = 9.88FGGGGAGQCVCGSRR111 pKa = 11.84AHH113 pKa = 6.31NNRR116 pKa = 3.4

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2721

67

2038

544.2

61.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.476 ± 1.834

2.573 ± 0.177

5.072 ± 0.519

7.24 ± 1.128

6.542 ± 0.783

5.954 ± 0.914

2.279 ± 0.715

5.917 ± 0.56

7.313 ± 0.709

10.254 ± 0.603

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.486

4.851 ± 0.236

3.602 ± 0.324

2.132 ± 0.387

4.778 ± 0.463

9.482 ± 0.818

3.969 ± 0.499

6.725 ± 1.005

0.992 ± 0.159

2.72 ± 0.769
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski