Rhizobium sp. KAs_5_22

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S6BID3|A0A2S6BID3_9RHIZ Uncharacterized protein OS=Rhizobium sp. KAs_5_22 OX=2067668 GN=C0075_16810 PE=4 SV=1
MM1 pKa = 6.85GQGMLSFSQRR11 pKa = 11.84TQTHH15 pKa = 5.31TKK17 pKa = 9.31LEE19 pKa = 4.15YY20 pKa = 10.91VRR22 pKa = 11.84VCDD25 pKa = 4.85AFGTGYY31 pKa = 10.47FYY33 pKa = 10.57IPGTEE38 pKa = 3.88TCLKK42 pKa = 9.42ISGYY46 pKa = 10.33VRR48 pKa = 11.84AQLDD52 pKa = 3.67WQNVQDD58 pKa = 4.8DD59 pKa = 4.34ADD61 pKa = 4.78GEE63 pKa = 4.32DD64 pKa = 4.05FDD66 pKa = 4.34WNARR70 pKa = 11.84TRR72 pKa = 11.84AKK74 pKa = 10.97LNFEE78 pKa = 4.58AKK80 pKa = 9.74NDD82 pKa = 3.97SEE84 pKa = 4.3IGTIGSYY91 pKa = 9.98IAIRR95 pKa = 11.84TWADD99 pKa = 2.63EE100 pKa = 4.65DD101 pKa = 4.44YY102 pKa = 11.28DD103 pKa = 4.24GGSLEE108 pKa = 4.31IDD110 pKa = 3.4EE111 pKa = 5.7AYY113 pKa = 8.44ITAAGFKK120 pKa = 9.71VGYY123 pKa = 8.81MYY125 pKa = 10.74NYY127 pKa = 9.05WDD129 pKa = 3.96NDD131 pKa = 3.44LSGEE135 pKa = 4.03TDD137 pKa = 3.32DD138 pKa = 5.97LGSNRR143 pKa = 11.84INSVGYY149 pKa = 9.89EE150 pKa = 4.02YY151 pKa = 11.2ASDD154 pKa = 3.65AFSAGVFIDD163 pKa = 3.98EE164 pKa = 5.43LSTNYY169 pKa = 10.27SGEE172 pKa = 4.13YY173 pKa = 9.75DD174 pKa = 3.46VHH176 pKa = 7.97GDD178 pKa = 3.41NDD180 pKa = 4.3SVGVEE185 pKa = 4.13AQVSATFGAITGEE198 pKa = 4.13LLGSYY203 pKa = 10.55DD204 pKa = 3.59FAAEE208 pKa = 3.96NGAVRR213 pKa = 11.84AKK215 pKa = 10.63LLAEE219 pKa = 4.23VGPGTVGLAAIYY231 pKa = 8.64STGANAYY238 pKa = 9.47YY239 pKa = 10.2DD240 pKa = 3.66VSEE243 pKa = 4.08WTVAAEE249 pKa = 3.97YY250 pKa = 10.29AIKK253 pKa = 10.96LNDD256 pKa = 4.07KK257 pKa = 10.76FSLTPGAQYY266 pKa = 9.53WFNYY270 pKa = 9.04GLVSADD276 pKa = 4.46EE277 pKa = 4.35FDD279 pKa = 4.06EE280 pKa = 6.44SNDD283 pKa = 2.87AWKK286 pKa = 10.73AGLTLDD292 pKa = 3.68YY293 pKa = 10.52KK294 pKa = 10.53IAEE297 pKa = 4.35GLTTKK302 pKa = 10.51VSVQYY307 pKa = 10.58FDD309 pKa = 4.99EE310 pKa = 5.08DD311 pKa = 3.84AADD314 pKa = 4.07DD315 pKa = 4.46GVWDD319 pKa = 3.96GFVRR323 pKa = 11.84LQRR326 pKa = 11.84SFF328 pKa = 3.42

Molecular weight: 36.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S6BKJ3|A0A2S6BKJ3_9RHIZ ArsR family transcriptional regulator OS=Rhizobium sp. KAs_5_22 OX=2067668 GN=C0075_20965 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.02GGRR28 pKa = 11.84KK29 pKa = 9.31VLVARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5237

0

5237

1493378

22

2832

285.2

31.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.092 ± 0.051

0.817 ± 0.011

5.734 ± 0.03

5.951 ± 0.033

3.915 ± 0.025

8.389 ± 0.03

2.037 ± 0.017

5.588 ± 0.028

3.695 ± 0.03

10.088 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.593 ± 0.017

2.735 ± 0.018

4.871 ± 0.03

2.98 ± 0.021

6.706 ± 0.033

5.617 ± 0.026

5.326 ± 0.025

7.356 ± 0.023

1.211 ± 0.014

2.299 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski