Burkholderia phage vB_BmuP_KL4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kelquatrovirus; Burkholderia virus KL4

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GN70|A0A2S1GN70_9CAUD Uncharacterized protein OS=Burkholderia phage vB_BmuP_KL4 OX=2115967 PE=4 SV=1
MM1 pKa = 7.65LNPRR5 pKa = 11.84LTEE8 pKa = 3.67RR9 pKa = 11.84AAEE12 pKa = 4.16FWSDD16 pKa = 3.11RR17 pKa = 11.84SKK19 pKa = 11.32RR20 pKa = 11.84EE21 pKa = 3.44YY22 pKa = 11.13DD23 pKa = 3.46DD24 pKa = 6.34AIDD27 pKa = 4.07AKK29 pKa = 10.89SDD31 pKa = 3.18EE32 pKa = 4.68AYY34 pKa = 10.66RR35 pKa = 11.84EE36 pKa = 4.09AFDD39 pKa = 5.18AGLAQAEE46 pKa = 4.18HH47 pKa = 7.14DD48 pKa = 3.73LVEE51 pKa = 4.37FAKK54 pKa = 10.78KK55 pKa = 9.93FVPDD59 pKa = 3.76PAEE62 pKa = 4.67SIDD65 pKa = 3.41ARR67 pKa = 11.84CRR69 pKa = 11.84RR70 pKa = 11.84FLAEE74 pKa = 3.89YY75 pKa = 9.83IGYY78 pKa = 9.71LDD80 pKa = 5.04CSWGDD85 pKa = 3.29LSAALASAAGLFQNDD100 pKa = 4.86DD101 pKa = 3.76GDD103 pKa = 3.78EE104 pKa = 4.15SS105 pKa = 3.78

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GN97|A0A2S1GN97_9CAUD Putative holin OS=Burkholderia phage vB_BmuP_KL4 OX=2115967 PE=4 SV=1
MM1 pKa = 7.73ADD3 pKa = 3.39EE4 pKa = 4.32TTKK7 pKa = 10.35PSRR10 pKa = 11.84NRR12 pKa = 11.84FRR14 pKa = 11.84KK15 pKa = 9.49IEE17 pKa = 3.8VRR19 pKa = 11.84MARR22 pKa = 11.84LNEE25 pKa = 3.92ALASAGAA32 pKa = 3.64

Molecular weight: 3.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13729

31

864

211.2

23.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.126 ± 0.673

1.085 ± 0.168

6.148 ± 0.335

5.179 ± 0.399

3.278 ± 0.214

8.296 ± 0.363

1.799 ± 0.161

4.531 ± 0.238

3.846 ± 0.287

8.209 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.629 ± 0.177

3.351 ± 0.275

4.749 ± 0.214

4.203 ± 0.342

7.116 ± 0.399

5.827 ± 0.383

5.951 ± 0.405

6.395 ± 0.213

1.741 ± 0.14

2.542 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski