Streptococcus phage CHPC1084

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FDQ2|A0A3G8FDQ2_9CAUD DUF3799 domain-containing protein OS=Streptococcus phage CHPC1084 OX=2365025 GN=CHPC1084_0036 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNWFVNHH14 pKa = 6.82RR15 pKa = 11.84GKK17 pKa = 9.04LTYY20 pKa = 11.06SMYY23 pKa = 10.8GSRR26 pKa = 11.84NGVDD30 pKa = 3.36GTADD34 pKa = 3.36CSGSISQALKK44 pKa = 9.74EE45 pKa = 4.19AGIGIQGLPSTVTLGQQLAKK65 pKa = 10.58NGFYY69 pKa = 10.18RR70 pKa = 11.84VSINQDD76 pKa = 2.14WDD78 pKa = 3.55ALTGDD83 pKa = 4.07IVLMSWGADD92 pKa = 2.98MSTSGGAGGHH102 pKa = 5.75VGVMMDD108 pKa = 3.33ATYY111 pKa = 9.9FISCDD116 pKa = 3.44YY117 pKa = 9.43STQGAPGQAINTYY130 pKa = 8.88PWNDD134 pKa = 3.08YY135 pKa = 10.1YY136 pKa = 11.51AANKK140 pKa = 6.83PTYY143 pKa = 9.85IEE145 pKa = 3.7VWRR148 pKa = 11.84YY149 pKa = 10.07ADD151 pKa = 5.12SAPQTNNKK159 pKa = 9.82ANTAVVPQSKK169 pKa = 10.2AYY171 pKa = 10.58YY172 pKa = 8.24EE173 pKa = 4.28ANEE176 pKa = 3.96VKK178 pKa = 10.25YY179 pKa = 11.21VNGIWQIRR187 pKa = 11.84CDD189 pKa = 4.05YY190 pKa = 7.72LTPIGFNFYY199 pKa = 11.14DD200 pKa = 3.52NGVPVDD206 pKa = 3.65MVNWVDD212 pKa = 4.64KK213 pKa = 10.87DD214 pKa = 3.93GNDD217 pKa = 4.47IPDD220 pKa = 4.25GDD222 pKa = 4.18SKK224 pKa = 11.5DD225 pKa = 4.0FKK227 pKa = 11.29SGMFFSFAGDD237 pKa = 3.56EE238 pKa = 4.15NNITDD243 pKa = 3.82TGEE246 pKa = 3.65GGYY249 pKa = 10.61YY250 pKa = 9.76GGYY253 pKa = 8.0YY254 pKa = 9.41YY255 pKa = 10.76RR256 pKa = 11.84RR257 pKa = 11.84FEE259 pKa = 4.06FGQFGTVWLSCWNKK273 pKa = 10.66DD274 pKa = 3.89DD275 pKa = 5.14LVNYY279 pKa = 7.38YY280 pKa = 8.26QQ281 pKa = 4.22

Molecular weight: 31.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FAS9|A0A3G8FAS9_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1084 OX=2365025 GN=CHPC1084_0039 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.75FGVSLLEE10 pKa = 4.01ARR12 pKa = 11.84RR13 pKa = 11.84MTLKK17 pKa = 10.49EE18 pKa = 3.53MKK20 pKa = 9.92LYY22 pKa = 10.32QKK24 pKa = 10.55AYY26 pKa = 9.84KK27 pKa = 10.03KK28 pKa = 10.52RR29 pKa = 11.84FLNKK33 pKa = 9.43EE34 pKa = 3.69RR35 pKa = 11.84EE36 pKa = 4.36IYY38 pKa = 9.68QLAYY42 pKa = 10.87LNRR45 pKa = 11.84LANATTKK52 pKa = 10.62DD53 pKa = 3.03GKK55 pKa = 10.64KK56 pKa = 10.46YY57 pKa = 10.49YY58 pKa = 10.19FEE60 pKa = 5.48KK61 pKa = 10.83FDD63 pKa = 3.95DD64 pKa = 4.49FYY66 pKa = 11.56NAKK69 pKa = 9.54EE70 pKa = 3.83RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.88VLGEE78 pKa = 4.45RR79 pKa = 11.84ITNSKK84 pKa = 10.22LLEE87 pKa = 4.03RR88 pKa = 11.84AKK90 pKa = 11.27NNLNYY95 pKa = 10.4KK96 pKa = 9.81LEE98 pKa = 4.57RR99 pKa = 11.84GLLDD103 pKa = 3.37GRR105 pKa = 4.46

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

11024

47

1545

234.6

26.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.52 ± 1.115

0.499 ± 0.101

6.159 ± 0.459

6.831 ± 0.822

4.327 ± 0.181

6.831 ± 0.532

1.433 ± 0.205

6.613 ± 0.357

7.856 ± 0.623

7.72 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.31

5.733 ± 0.415

2.758 ± 0.265

4.173 ± 0.257

4.3 ± 0.425

6.903 ± 0.656

6.431 ± 0.347

6.468 ± 0.305

1.179 ± 0.174

3.855 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski