Streptococcus satellite phage Javan86
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZXQ5|A0A4D5ZXQ5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan86 OX=2558865 GN=JavanS86_0017 PE=4 SV=1
MM1 pKa = 7.63 TDD3 pKa = 3.77 KK4 pKa = 10.59 KK5 pKa = 9.49 TIDD8 pKa = 3.59 LNNYY12 pKa = 9.36 GITDD16 pKa = 3.58 KK17 pKa = 11.55 DD18 pKa = 3.64 GDD20 pKa = 4.29 DD21 pKa = 3.33 VTFYY25 pKa = 11.09 QIANFYY31 pKa = 11.25 NSFLEE36 pKa = 5.17 VADD39 pKa = 3.9 HH40 pKa = 6.74 WLDD43 pKa = 4.03 AEE45 pKa = 4.43 LTDD48 pKa = 4.17 GQAWRR53 pKa = 11.84 YY54 pKa = 7.52 MMQIYY59 pKa = 10.59 AEE61 pKa = 4.19 LFLYY65 pKa = 10.29 PEE67 pKa = 5.16 DD68 pKa = 4.37 EE69 pKa = 4.37 PASTEE74 pKa = 3.87 EE75 pKa = 4.09 TTAA78 pKa = 3.47
Molecular weight: 9.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.745
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.63
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A4D5ZWR1|A0A4D5ZWR1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan86 OX=2558865 GN=JavanS86_0009 PE=4 SV=1
MM1 pKa = 7.65 TIKK4 pKa = 10.49 QFTKK8 pKa = 10.54 KK9 pKa = 10.34 NGEE12 pKa = 3.88 TVYY15 pKa = 10.45 RR16 pKa = 11.84 QRR18 pKa = 11.84 VYY20 pKa = 11.13 LGTDD24 pKa = 2.68 SMTGKK29 pKa = 9.73 QVQVTITGRR38 pKa = 11.84 TKK40 pKa = 10.87 SEE42 pKa = 3.92 VSQKK46 pKa = 10.64 AIKK49 pKa = 10.18 KK50 pKa = 9.95 LADD53 pKa = 3.75 FQANGSTVLKK63 pKa = 8.65 MQQVNNYY70 pKa = 9.31 SDD72 pKa = 5.97 LVTLWLSSYY81 pKa = 10.83 KK82 pKa = 10.06 LTVKK86 pKa = 9.91 PQTYY90 pKa = 8.71 RR91 pKa = 11.84 HH92 pKa = 5.32 TEE94 pKa = 3.64 ITIHH98 pKa = 5.86 KK99 pKa = 9.4 HH100 pKa = 5.69 LIPFFGTMKK109 pKa = 10.39 LEE111 pKa = 4.65 KK112 pKa = 9.79 ISMPFVQYY120 pKa = 9.53 FANSISSSGLKK131 pKa = 9.53 EE132 pKa = 3.99 YY133 pKa = 10.95 KK134 pKa = 10.24 PIIALNKK141 pKa = 10.1 RR142 pKa = 11.84 ILQYY146 pKa = 10.9 AVHH149 pKa = 6.14 LQIITRR155 pKa = 11.84 NPADD159 pKa = 4.25 NIIVPCMRR167 pKa = 11.84 QEE169 pKa = 4.16 EE170 pKa = 4.64 KK171 pKa = 10.57 NLKK174 pKa = 10.08 YY175 pKa = 10.74 LDD177 pKa = 4.47 DD178 pKa = 4.21 SQLKK182 pKa = 9.46 QFIGYY187 pKa = 9.78 LDD189 pKa = 4.16 SLPDD193 pKa = 3.58 NYY195 pKa = 10.77 KK196 pKa = 10.4 NNYY199 pKa = 8.48 NRR201 pKa = 11.84 VLYY204 pKa = 10.36 QFLLATGLRR213 pKa = 11.84 IGEE216 pKa = 4.54 AIALEE221 pKa = 4.14 WSDD224 pKa = 3.66 IDD226 pKa = 5.51 LEE228 pKa = 4.47 NGTINITKK236 pKa = 8.95 TFNPVINKK244 pKa = 9.87 LSTPKK249 pKa = 9.78 TKK251 pKa = 10.37 AGKK254 pKa = 9.83 RR255 pKa = 11.84 VISIDD260 pKa = 3.58 RR261 pKa = 11.84 KK262 pKa = 9.09 SLLMLRR268 pKa = 11.84 LYY270 pKa = 10.39 KK271 pKa = 10.22 VRR273 pKa = 11.84 QEE275 pKa = 4.08 QKK277 pKa = 8.68 FKK279 pKa = 11.4 EE280 pKa = 4.24 MISPYY285 pKa = 10.42 GNYY288 pKa = 9.53 VFSNGLAPYY297 pKa = 9.29 PFRR300 pKa = 11.84 NNLQRR305 pKa = 11.84 MLDD308 pKa = 3.56 NHH310 pKa = 6.67 LEE312 pKa = 4.18 RR313 pKa = 11.84 AGLPRR318 pKa = 11.84 FTFHH322 pKa = 7.9 AFRR325 pKa = 11.84 HH326 pKa = 4.69 THH328 pKa = 6.92 ASLLLNAGISYY339 pKa = 10.65 KK340 pKa = 10.3 EE341 pKa = 3.81 LQHH344 pKa = 7.01 RR345 pKa = 11.84 LGHH348 pKa = 5.9 SKK350 pKa = 10.3 ISMTLDD356 pKa = 3.44 VYY358 pKa = 11.48 SHH360 pKa = 6.96 LSKK363 pKa = 10.93 SKK365 pKa = 9.49 EE366 pKa = 4.09 KK367 pKa = 10.76 EE368 pKa = 3.62 AVTYY372 pKa = 9.03 YY373 pKa = 10.81 EE374 pKa = 4.2 KK375 pKa = 11.2 ALGKK379 pKa = 10.25 LL380 pKa = 3.53
Molecular weight: 43.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.114
IPC2_protein 9.385
IPC_protein 9.311
Toseland 9.97
ProMoST 9.663
Dawson 10.189
Bjellqvist 9.853
Wikipedia 10.35
Rodwell 10.672
Grimsley 10.262
Solomon 10.204
Lehninger 10.16
Nozaki 9.94
DTASelect 9.853
Thurlkill 10.028
EMBOSS 10.379
Sillero 10.087
Patrickios 10.072
IPC_peptide 10.204
IPC2_peptide 8.361
IPC2.peptide.svr19 8.354
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2547
65
475
149.8
17.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.575 ± 0.527
0.51 ± 0.153
5.693 ± 0.578
8.638 ± 0.714
3.691 ± 0.305
4.947 ± 0.422
1.413 ± 0.316
6.243 ± 0.396
8.716 ± 0.5
10.561 ± 0.583
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.866 ± 0.415
5.575 ± 0.542
2.67 ± 0.325
4.476 ± 0.491
5.457 ± 0.62
5.732 ± 0.29
6.243 ± 0.373
4.751 ± 0.353
0.942 ± 0.216
5.3 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here