Lentibacillus cibarius
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3079 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A549YJV1|A0A549YJV1_9BACI MATE family efflux transporter OS=Lentibacillus cibarius OX=2583219 GN=FH966_10990 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.4 KK3 pKa = 10.49 GLLAMLVLGLALFLAACGGGDD24 pKa = 3.81 SNDD27 pKa = 3.77 SNGSDD32 pKa = 3.28 EE33 pKa = 4.3 TGNDD37 pKa = 3.34 GGDD40 pKa = 3.43 AAEE43 pKa = 4.4 TAGSTMDD50 pKa = 3.24 VTATNFSFDD59 pKa = 3.48 KK60 pKa = 11.16 DD61 pKa = 3.92 EE62 pKa = 4.27 YY63 pKa = 9.49 TVPAGEE69 pKa = 4.42 EE70 pKa = 4.26 VTVSLTNEE78 pKa = 3.91 EE79 pKa = 4.08 GMHH82 pKa = 6.86 GIGIDD87 pKa = 3.5 EE88 pKa = 4.46 FDD90 pKa = 3.85 VNIEE94 pKa = 4.21 GEE96 pKa = 4.49 GEE98 pKa = 4.0 ATFTPEE104 pKa = 3.8 EE105 pKa = 4.23 PGEE108 pKa = 3.94 YY109 pKa = 9.11 TIYY112 pKa = 10.81 CSVPCGQGHH121 pKa = 7.53 ADD123 pKa = 3.33 MKK125 pKa = 9.99 STLVVEE131 pKa = 4.81
Molecular weight: 13.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.681
IPC_protein 3.63
Toseland 3.439
ProMoST 3.757
Dawson 3.605
Bjellqvist 3.821
Wikipedia 3.528
Rodwell 3.465
Grimsley 3.35
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.884
Thurlkill 3.49
EMBOSS 3.541
Sillero 3.745
Patrickios 0.846
IPC_peptide 3.592
IPC2_peptide 3.732
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A549YGW5|A0A549YGW5_9BACI Sporulation protein YabP OS=Lentibacillus cibarius OX=2583219 GN=yabP PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.42 RR3 pKa = 11.84 PFQPNNRR10 pKa = 11.84 KK11 pKa = 9.19 RR12 pKa = 11.84 KK13 pKa = 8.49 KK14 pKa = 8.63 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 KK20 pKa = 10.0 RR21 pKa = 11.84 MSTKK25 pKa = 10.48 DD26 pKa = 2.94 GRR28 pKa = 11.84 QVLKK32 pKa = 10.25 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.96 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.428
IPC2_protein 10.921
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.457
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.179
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.058
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3079
0
3079
883714
26
1431
287.0
32.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.431 ± 0.047
0.678 ± 0.014
5.768 ± 0.039
7.168 ± 0.054
4.31 ± 0.041
6.939 ± 0.045
2.189 ± 0.021
7.414 ± 0.044
6.357 ± 0.043
9.362 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.054 ± 0.025
4.437 ± 0.033
3.697 ± 0.03
4.01 ± 0.036
4.172 ± 0.032
5.805 ± 0.032
5.701 ± 0.023
7.043 ± 0.039
1.029 ± 0.016
3.436 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here