Yersinia phage YeP1
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6B577|A0A5J6B577_9CAUD SOS-response repressor and protease OS=Yersinia phage YeP1 OX=2562809 PE=3 SV=1
MM1 pKa = 7.54 ANVEE5 pKa = 4.57 AKK7 pKa = 9.82 WSVDD11 pKa = 3.78 LYY13 pKa = 10.94 VEE15 pKa = 4.55 CPKK18 pKa = 11.09 CGDD21 pKa = 3.71 FFDD24 pKa = 5.69 LNSDD28 pKa = 3.69 DD29 pKa = 4.07 TFTDD33 pKa = 3.99 GTHH36 pKa = 6.97 GYY38 pKa = 10.56 ALQTTVDD45 pKa = 3.57 VDD47 pKa = 4.11 AEE49 pKa = 4.29 CPEE52 pKa = 4.77 CGHH55 pKa = 6.49 KK56 pKa = 10.58 FKK58 pKa = 11.29 ADD60 pKa = 3.87 LVWW63 pKa = 5.16
Molecular weight: 7.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 4.037
IPC_protein 3.961
Toseland 3.757
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.342
Thurlkill 3.834
EMBOSS 3.935
Sillero 4.088
Patrickios 0.846
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.965
Protein with the highest isoelectric point:
>tr|A0A5K1KD70|A0A5K1KD70_9CAUD Uncharacterized protein OS=Yersinia phage YeP1 OX=2562809 PE=4 SV=1
MM1 pKa = 7.59 GIEE4 pKa = 4.1 NSTSGLYY11 pKa = 10.44 LHH13 pKa = 7.14 VDD15 pKa = 4.22 FDD17 pKa = 4.23 KK18 pKa = 11.3 PKK20 pKa = 10.47 EE21 pKa = 3.94 LEE23 pKa = 4.13 FNQKK27 pKa = 8.43 RR28 pKa = 11.84 VRR30 pKa = 11.84 NAFAKK35 pKa = 10.18 VGRR38 pKa = 11.84 GVQDD42 pKa = 3.14 EE43 pKa = 4.02 ARR45 pKa = 11.84 RR46 pKa = 11.84 LVARR50 pKa = 11.84 HH51 pKa = 6.53 AISKK55 pKa = 10.37 AGEE58 pKa = 4.01 APGTRR63 pKa = 11.84 TGGLSKK69 pKa = 10.82 SIGYY73 pKa = 8.76 KK74 pKa = 9.99 VPSPSGGRR82 pKa = 11.84 PGFMVRR88 pKa = 11.84 IAPNQKK94 pKa = 8.95 GGRR97 pKa = 11.84 RR98 pKa = 11.84 ATRR101 pKa = 11.84 LPSGDD106 pKa = 3.89 DD107 pKa = 3.83 FYY109 pKa = 11.08 PAFLFYY115 pKa = 10.7 GVKK118 pKa = 10.27 SKK120 pKa = 11.17 AKK122 pKa = 9.74 RR123 pKa = 11.84 GRR125 pKa = 11.84 SHH127 pKa = 7.37 RR128 pKa = 11.84 KK129 pKa = 8.12 GASGGSGWKK138 pKa = 9.17 IAPRR142 pKa = 11.84 KK143 pKa = 10.32 NFMTEE148 pKa = 3.29 ALEE151 pKa = 4.43 NRR153 pKa = 11.84 RR154 pKa = 11.84 LWAEE158 pKa = 3.89 KK159 pKa = 10.88 VLFSALKK166 pKa = 9.81 KK167 pKa = 10.29 SVVVKK172 pKa = 10.76
Molecular weight: 18.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 9.984
IPC_protein 10.95
Toseland 11.228
ProMoST 11.213
Dawson 11.272
Bjellqvist 11.052
Wikipedia 11.55
Rodwell 11.506
Grimsley 11.301
Solomon 11.52
Lehninger 11.477
Nozaki 11.213
DTASelect 11.052
Thurlkill 11.213
EMBOSS 11.667
Sillero 11.228
Patrickios 11.228
IPC_peptide 11.535
IPC2_peptide 10.072
IPC2.peptide.svr19 8.584
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
12052
38
626
197.6
21.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.102 ± 0.417
1.278 ± 0.163
5.899 ± 0.262
6.057 ± 0.278
3.626 ± 0.243
7.393 ± 0.232
1.676 ± 0.168
6.09 ± 0.22
5.551 ± 0.394
9.003 ± 0.325
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.746 ± 0.176
4.24 ± 0.163
4.588 ± 0.212
3.784 ± 0.231
5.451 ± 0.32
6.256 ± 0.244
6.115 ± 0.305
6.605 ± 0.247
1.518 ± 0.131
3.02 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here