Saccharothrix espanaensis (strain ATCC 51144 / DSM 44229 / JCM 9112 / NBRC 15066 / NRRL 15764)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharothrix; Saccharothrix espanaensis

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8423 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0K5K3|K0K5K3_SACES Guanylate kinase OS=Saccharothrix espanaensis (strain ATCC 51144 / DSM 44229 / JCM 9112 / NBRC 15066 / NRRL 15764) OX=1179773 GN=gmk PE=3 SV=1
MM1 pKa = 7.6SVEE4 pKa = 3.93TFQRR8 pKa = 11.84SRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84GGWHH17 pKa = 6.56RR18 pKa = 11.84RR19 pKa = 11.84LAMTAAVSVASAGLLAVGAQPASAAVVTHH48 pKa = 6.3PFAYY52 pKa = 9.89VANSGANTVTAYY64 pKa = 10.23DD65 pKa = 3.46ATTATVASTIVVGSTPLGLVATPDD89 pKa = 3.39GARR92 pKa = 11.84VYY94 pKa = 9.9VAKK97 pKa = 10.77LSGGVSVIDD106 pKa = 3.61TAAGAVVASIAVGSQPIGIAITVDD130 pKa = 3.09GTRR133 pKa = 11.84ILVVNSGSNSVSMIDD148 pKa = 3.33TATNAVTATVTVGANPTSVAISPDD172 pKa = 3.03GATAYY177 pKa = 7.83VTNDD181 pKa = 2.63ASGTVSVIDD190 pKa = 3.61TATATVTGTINLGLTQPAGVAITPDD215 pKa = 3.21GATVYY220 pKa = 9.7VARR223 pKa = 11.84YY224 pKa = 8.3GAGTVAVIDD233 pKa = 3.87TATNAVTGSITVGAVPSGVAVSPDD257 pKa = 3.0GTRR260 pKa = 11.84AYY262 pKa = 9.76VANIGPDD269 pKa = 3.76TISVIDD275 pKa = 3.8TATDD279 pKa = 3.38TVTATVPVGSNPSRR293 pKa = 11.84VAVTDD298 pKa = 4.1DD299 pKa = 3.23GSTVYY304 pKa = 9.39ATNNGAGSVAAINTATNTVTTTFAVGSGPFGVTTANVVQPRR345 pKa = 11.84VTAISPTTGPTAGGTSVTITGTSLAAATAVRR376 pKa = 11.84FGATPATSFTAVNDD390 pKa = 3.91TTVTATAPAHH400 pKa = 5.9AAGPVDD406 pKa = 3.78VTVTTPTGVSTTSAADD422 pKa = 3.08QYY424 pKa = 11.03TYY426 pKa = 11.24LDD428 pKa = 4.1PAPVISAVDD437 pKa = 3.73PATGPAAGGTPVTITGTHH455 pKa = 4.92FTGATAVAFGGTPATSFTVVNDD477 pKa = 3.57TSITATAPAGTAGTVDD493 pKa = 3.44VTVTNAGGTSAPGSGYY509 pKa = 9.42TYY511 pKa = 10.27VAAPAVTALDD521 pKa = 3.91PTSGPTAGGTAVAITGTALTGTTAVRR547 pKa = 11.84FDD549 pKa = 3.98GVDD552 pKa = 3.03ATSFTVTSSTSITAVAPAHH571 pKa = 5.96SAGTVDD577 pKa = 3.43VTVTTVGGTSAATASSRR594 pKa = 11.84YY595 pKa = 7.93TYY597 pKa = 11.4ADD599 pKa = 3.33ADD601 pKa = 4.04LSVSVTDD608 pKa = 3.64SADD611 pKa = 3.59PVSLGGDD618 pKa = 3.72PYY620 pKa = 10.8TYY622 pKa = 10.66TVTAANAGPSTATGVTVSTTLSGASTTINSATPSQGSCTTGAATVSCTLGTIAGSGSASVTISVTPSTSGTATATAAVSATEE704 pKa = 4.21PDD706 pKa = 3.82PVPANNTDD714 pKa = 3.8TEE716 pKa = 4.57STTVDD721 pKa = 3.1NALGCTIVGTAGNDD735 pKa = 3.55SLNGTNGNDD744 pKa = 3.81VICGLGGNDD753 pKa = 4.5TITGGNGTDD762 pKa = 3.85TIHH765 pKa = 7.2AGTGDD770 pKa = 3.74DD771 pKa = 3.99VLVGGNGADD780 pKa = 3.33TLYY783 pKa = 11.23GGLGNDD789 pKa = 3.46TSKK792 pKa = 11.54GEE794 pKa = 4.11SLLDD798 pKa = 3.53SLLGLFDD805 pKa = 5.09NGDD808 pKa = 3.62DD809 pKa = 4.53TIDD812 pKa = 4.03GGPGNDD818 pKa = 4.46NLDD821 pKa = 3.81GQNGNDD827 pKa = 3.72TLIDD831 pKa = 3.68HH832 pKa = 7.56DD833 pKa = 4.74GTDD836 pKa = 3.52TMAGSLGNDD845 pKa = 3.78TIDD848 pKa = 3.45VQDD851 pKa = 4.96GIGGDD856 pKa = 3.77TANGGLGTDD865 pKa = 3.33TCTVDD870 pKa = 4.72GGDD873 pKa = 3.74TTSSCC878 pKa = 4.44

Molecular weight: 83.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0KCC7|K0KCC7_SACES ABC-type transporter substrate-binding lipoprotein OS=Saccharothrix espanaensis (strain ATCC 51144 / DSM 44229 / JCM 9112 / NBRC 15066 / NRRL 15764) OX=1179773 GN=BN6_79590 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.77GRR42 pKa = 11.84QQLSAA47 pKa = 4.05

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8423

0

8423

2787681

27

6716

331.0

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.441 ± 0.043

0.793 ± 0.008

6.286 ± 0.025

5.316 ± 0.021

2.796 ± 0.015

9.338 ± 0.027

2.349 ± 0.015

2.791 ± 0.018

1.853 ± 0.02

10.491 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.471 ± 0.011

1.773 ± 0.019

6.154 ± 0.026

2.573 ± 0.016

8.467 ± 0.031

4.895 ± 0.02

6.082 ± 0.037

9.716 ± 0.027

1.547 ± 0.011

1.869 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski