Bos taurus papillomavirus 13
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|J9V4T2|J9V4T2_BPV1 Protein E6 OS=Bos taurus papillomavirus 13 OX=1887213 GN=E6 PE=3 SV=1
MM1 pKa = 7.49 PNLWFLLFLGLVAAMQLLLLLFLLLFFLVYY31 pKa = 9.32 WDD33 pKa = 5.61 HH34 pKa = 7.17 FDD36 pKa = 4.07 CSCTGLPFF44 pKa = 4.63
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.246
IPC2_protein 4.647
IPC_protein 3.923
Toseland 3.783
ProMoST 4.24
Dawson 4.012
Bjellqvist 4.202
Wikipedia 4.113
Rodwell 3.821
Grimsley 3.732
Solomon 3.923
Lehninger 3.872
Nozaki 4.228
DTASelect 4.431
Thurlkill 3.973
EMBOSS 4.101
Sillero 4.101
Patrickios 0.006
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 4.02
Protein with the highest isoelectric point:
>tr|J9V8D6|J9V8D6_BPV1 Protein E7 OS=Bos taurus papillomavirus 13 OX=1887213 GN=E7 PE=3 SV=1
MM1 pKa = 7.21 LVSHH5 pKa = 7.33 PPLLILEE12 pKa = 4.73 IAQTEE17 pKa = 4.58 SGSHH21 pKa = 4.82 QKK23 pKa = 10.32 DD24 pKa = 3.33 LKK26 pKa = 10.22 EE27 pKa = 3.93 TLQEE31 pKa = 4.37 KK32 pKa = 10.44 KK33 pKa = 10.02 PSQPSLSLLCSAPPPAVPSEE53 pKa = 4.08 QASVGYY59 pKa = 8.37 GTVLARR65 pKa = 11.84 TPTIFLQARR74 pKa = 11.84 GALFSALPPPRR85 pKa = 11.84 CRR87 pKa = 11.84 ARR89 pKa = 11.84 YY90 pKa = 8.6 RR91 pKa = 11.84 WTWHH95 pKa = 4.93 QGRR98 pKa = 11.84 KK99 pKa = 8.84 KK100 pKa = 10.52 KK101 pKa = 8.97 KK102 pKa = 7.05 TNRR105 pKa = 11.84 PTPQRR110 pKa = 11.84 RR111 pKa = 11.84 NLL113 pKa = 3.44
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.794
IPC_protein 10.599
Toseland 10.994
ProMoST 10.701
Dawson 11.038
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 11.316
Grimsley 11.067
Solomon 11.213
Lehninger 11.169
Nozaki 10.965
DTASelect 10.745
Thurlkill 10.965
EMBOSS 11.389
Sillero 10.979
Patrickios 11.067
IPC_peptide 11.213
IPC2_peptide 9.794
IPC2.peptide.svr19 8.594
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2406
44
606
300.8
33.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.855 ± 0.55
2.494 ± 0.635
5.278 ± 0.377
5.985 ± 0.535
4.073 ± 0.557
7.481 ± 0.787
2.411 ± 0.182
3.699 ± 0.718
5.362 ± 0.84
9.767 ± 0.889
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.33 ± 0.22
3.533 ± 0.53
6.151 ± 1.109
4.115 ± 0.471
5.943 ± 0.537
7.689 ± 0.611
6.65 ± 0.554
5.569 ± 0.334
1.496 ± 0.242
3.117 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here