Hubei sobemo-like virus 16

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KET4|A0A1L3KET4_9VIRU RdRP_1 domain-containing protein OS=Hubei sobemo-like virus 16 OX=1923201 PE=4 SV=1
MM1 pKa = 6.94PTVSIRR7 pKa = 11.84LPSLMDD13 pKa = 3.2VALLVVGTLYY23 pKa = 11.37LWMSWGVMTAPNAPAGVVGQNILTIVVFGFLYY55 pKa = 10.64LRR57 pKa = 11.84TGFASSGRR65 pKa = 11.84KK66 pKa = 8.74VVHH69 pKa = 5.6QVAKK73 pKa = 10.03SVSVLEE79 pKa = 4.06STIPGSHH86 pKa = 6.35IMEE89 pKa = 3.81VDD91 pKa = 4.55FKK93 pKa = 11.45NRR95 pKa = 11.84SLPKK99 pKa = 9.49CQTVVYY105 pKa = 10.47QEE107 pKa = 4.46TEE109 pKa = 3.76DD110 pKa = 4.41GEE112 pKa = 4.41YY113 pKa = 10.63HH114 pKa = 7.07LLGNVVRR121 pKa = 11.84IGDD124 pKa = 3.98FLVMPRR130 pKa = 11.84HH131 pKa = 5.84IADD134 pKa = 3.53VAKK137 pKa = 10.02EE138 pKa = 3.63LYY140 pKa = 9.56IAEE143 pKa = 4.37RR144 pKa = 11.84EE145 pKa = 4.28NFDD148 pKa = 4.04SVHH151 pKa = 4.65QVKK154 pKa = 10.26RR155 pKa = 11.84DD156 pKa = 3.27RR157 pKa = 11.84WEE159 pKa = 4.49DD160 pKa = 3.25LATDD164 pKa = 4.38LVYY167 pKa = 10.36MRR169 pKa = 11.84ASADD173 pKa = 3.77LLSTLQLSKK182 pKa = 11.36ASIGQLTTGIVASISTAAPSEE203 pKa = 4.17DD204 pKa = 3.4KK205 pKa = 10.98LLYY208 pKa = 9.49SCGVARR214 pKa = 11.84PSKK217 pKa = 10.65DD218 pKa = 2.61IFGLVEE224 pKa = 4.1YY225 pKa = 10.31KK226 pKa = 10.86GSTRR230 pKa = 11.84GGFSGAGLFVGTQLIGIHH248 pKa = 5.08YY249 pKa = 10.27QGTPSVNFSYY259 pKa = 10.29EE260 pKa = 3.56ASYY263 pKa = 10.45VKK265 pKa = 10.91AMLPRR270 pKa = 11.84EE271 pKa = 4.11EE272 pKa = 4.49ASDD275 pKa = 3.49WEE277 pKa = 4.36TAQWLEE283 pKa = 5.04DD284 pKa = 3.82IISKK288 pKa = 10.16KK289 pKa = 10.81GKK291 pKa = 9.35LKK293 pKa = 10.94AMRR296 pKa = 11.84DD297 pKa = 3.61PSDD300 pKa = 3.67PSDD303 pKa = 3.02WLVYY307 pKa = 10.76AGGKK311 pKa = 5.78YY312 pKa = 10.01HH313 pKa = 7.43RR314 pKa = 11.84IEE316 pKa = 5.52GDD318 pKa = 3.51VMSKK322 pKa = 10.95YY323 pKa = 10.53EE324 pKa = 4.19DD325 pKa = 3.87YY326 pKa = 11.77VDD328 pKa = 4.68FGYY331 pKa = 10.7DD332 pKa = 3.08EE333 pKa = 4.33PVARR337 pKa = 11.84RR338 pKa = 11.84EE339 pKa = 3.85MRR341 pKa = 11.84EE342 pKa = 3.38YY343 pKa = 10.98DD344 pKa = 3.33APHH347 pKa = 6.69YY348 pKa = 10.54SNQDD352 pKa = 2.97FRR354 pKa = 11.84VPPGLGNQEE363 pKa = 4.01ASHH366 pKa = 6.0AQPVCKK372 pKa = 10.22NLEE375 pKa = 3.81QLNILRR381 pKa = 11.84EE382 pKa = 4.16DD383 pKa = 3.71ADD385 pKa = 3.78NAKK388 pKa = 10.25QEE390 pKa = 4.08IDD392 pKa = 3.6DD393 pKa = 4.17LANVVDD399 pKa = 4.78EE400 pKa = 4.54VLHH403 pKa = 5.49AQKK406 pKa = 10.0ATVGSLEE413 pKa = 4.1EE414 pKa = 4.32LKK416 pKa = 10.76KK417 pKa = 10.35QQQEE421 pKa = 4.0LKK423 pKa = 10.65ALLRR427 pKa = 11.84KK428 pKa = 8.29LTQLPPTKK436 pKa = 10.05EE437 pKa = 3.92KK438 pKa = 10.57GVKK441 pKa = 8.29IHH443 pKa = 6.72SVEE446 pKa = 4.02EE447 pKa = 3.85QLNSNKK453 pKa = 10.36GEE455 pKa = 4.04IKK457 pKa = 10.53KK458 pKa = 10.0LANVPASSKK467 pKa = 10.68SPKK470 pKa = 8.28KK471 pKa = 8.76TKK473 pKa = 8.77TKK475 pKa = 9.58RR476 pKa = 11.84THH478 pKa = 6.22QDD480 pKa = 3.83LIQNLSPEE488 pKa = 4.38QIQAVCVINSNPEE501 pKa = 4.03LFRR504 pKa = 11.84VTQAALNNGEE514 pKa = 4.16VMQAILSRR522 pKa = 11.84LSQGRR527 pKa = 11.84NSISTSSSS535 pKa = 2.77

Molecular weight: 59.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KET4|A0A1L3KET4_9VIRU RdRP_1 domain-containing protein OS=Hubei sobemo-like virus 16 OX=1923201 PE=4 SV=1
MM1 pKa = 7.78LDD3 pKa = 2.85KK4 pKa = 10.53AYY6 pKa = 10.59RR7 pKa = 11.84IIHH10 pKa = 5.64GVSIIDD16 pKa = 3.58NLVARR21 pKa = 11.84LLFGNQFDD29 pKa = 4.21NLISNWSVLPSKK41 pKa = 10.92VGWTPGQGGYY51 pKa = 10.71KK52 pKa = 10.25LLAKK56 pKa = 9.97KK57 pKa = 10.45YY58 pKa = 8.65PYY60 pKa = 10.91SMSADD65 pKa = 3.07KK66 pKa = 11.1SAWDD70 pKa = 3.25WTMQPWCVDD79 pKa = 2.78LMIAMLRR86 pKa = 11.84NLGPQDD92 pKa = 3.12EE93 pKa = 4.79DD94 pKa = 4.02YY95 pKa = 11.31SRR97 pKa = 11.84RR98 pKa = 11.84VEE100 pKa = 3.92NHH102 pKa = 5.38LVALFGIGAKK112 pKa = 10.26FIAGNTVFTQRR123 pKa = 11.84VQGIMKK129 pKa = 9.96SGYY132 pKa = 10.33LGTIHH137 pKa = 7.03LNSILQTALHH147 pKa = 6.45ILAKK151 pKa = 10.01IRR153 pKa = 11.84LGQDD157 pKa = 2.94VEE159 pKa = 4.66PYY161 pKa = 9.04PDD163 pKa = 3.54SVGDD167 pKa = 3.77DD168 pKa = 3.77TIQRR172 pKa = 11.84PEE174 pKa = 4.56ADD176 pKa = 3.36PEE178 pKa = 4.73TYY180 pKa = 10.17FRR182 pKa = 11.84TLEE185 pKa = 4.3LGGCVIKK192 pKa = 10.64QYY194 pKa = 9.47EE195 pKa = 4.39TVKK198 pKa = 10.59GVDD201 pKa = 4.02FVGHH205 pKa = 6.97RR206 pKa = 11.84FNEE209 pKa = 4.42QQCLPNYY216 pKa = 9.71RR217 pKa = 11.84SKK219 pKa = 10.17HH220 pKa = 5.3CYY222 pKa = 9.75RR223 pKa = 11.84LFHH226 pKa = 7.86AEE228 pKa = 4.0DD229 pKa = 3.37QLVPEE234 pKa = 4.35TLEE237 pKa = 4.77GYY239 pKa = 10.09QYY241 pKa = 11.22LYY243 pKa = 10.52PHH245 pKa = 6.6DD246 pKa = 4.08TKK248 pKa = 10.79FLKK251 pKa = 10.5AIHH254 pKa = 6.57SMMVDD259 pKa = 3.66CDD261 pKa = 3.29AVDD264 pKa = 3.73RR265 pKa = 11.84MRR267 pKa = 11.84SPSYY271 pKa = 10.21LRR273 pKa = 11.84YY274 pKa = 8.69WYY276 pKa = 9.94DD277 pKa = 3.33YY278 pKa = 11.6AVV280 pKa = 3.16

Molecular weight: 32.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

815

280

535

407.5

45.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.871 ± 0.662

1.104 ± 0.39

6.012 ± 0.647

5.767 ± 1.052

2.822 ± 0.429

6.748 ± 0.226

2.577 ± 0.365

5.153 ± 0.321

6.012 ± 0.579

9.816 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.365

3.804 ± 0.133

4.54 ± 0.059

5.153 ± 0.088

4.908 ± 0.257

7.607 ± 1.287

4.54 ± 0.145

8.098 ± 0.751

1.472 ± 0.384

4.417 ± 1.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski