Pedobacter xixiisoli

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286ADX5|A0A286ADX5_9SPHI AraC-type DNA-binding protein OS=Pedobacter xixiisoli OX=1476464 GN=SAMN06297358_3817 PE=4 SV=1
MM1 pKa = 7.68SDD3 pKa = 2.94INIVIEE9 pKa = 4.18DD10 pKa = 3.94RR11 pKa = 11.84EE12 pKa = 4.59GNALDD17 pKa = 4.41HH18 pKa = 6.28VAPTDD23 pKa = 3.39MGLSLMEE30 pKa = 3.86FLKK33 pKa = 10.93ASEE36 pKa = 4.22YY37 pKa = 11.12DD38 pKa = 3.59VLATCGGMALCATCCVDD55 pKa = 3.54VLEE58 pKa = 5.31GEE60 pKa = 4.35EE61 pKa = 4.53KK62 pKa = 10.73LNEE65 pKa = 4.07MSDD68 pKa = 3.95DD69 pKa = 3.99EE70 pKa = 4.73YY71 pKa = 11.97AMLDD75 pKa = 3.8TLPDD79 pKa = 4.06LLPNSRR85 pKa = 11.84LACQIQLSPAMEE97 pKa = 4.18GLKK100 pKa = 10.4VRR102 pKa = 11.84LHH104 pKa = 6.79AEE106 pKa = 3.79QQ107 pKa = 4.04

Molecular weight: 11.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285ZW68|A0A285ZW68_9SPHI Bacteroides conjugative transposon TraK protein OS=Pedobacter xixiisoli OX=1476464 GN=SAMN06297358_1287 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84VFGRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.74KK9 pKa = 10.97LEE11 pKa = 4.16LSSSQDD17 pKa = 2.93KK18 pKa = 10.28LAQRR22 pKa = 11.84MADD25 pKa = 4.14RR26 pKa = 11.84ICRR29 pKa = 11.84LQRR32 pKa = 11.84KK33 pKa = 8.3IADD36 pKa = 3.88DD37 pKa = 4.33LNVRR41 pKa = 11.84TAGWGRR47 pKa = 11.84RR48 pKa = 11.84SWLVLLVLLCLLMGGYY64 pKa = 9.29CAYY67 pKa = 10.86LVIDD71 pKa = 4.52ALNN74 pKa = 3.34

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4166

0

4166

1461943

29

3855

350.9

39.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.502 ± 0.042

0.707 ± 0.012

5.194 ± 0.029

5.888 ± 0.048

5.038 ± 0.028

6.692 ± 0.042

1.677 ± 0.017

7.331 ± 0.041

7.555 ± 0.047

9.465 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.017

6.098 ± 0.039

3.486 ± 0.018

3.68 ± 0.025

3.603 ± 0.024

6.422 ± 0.034

5.919 ± 0.046

6.429 ± 0.032

1.094 ± 0.012

4.089 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski