Stx converting phage vB_EcoS_P27

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Traversvirus; Escherichia virus P27

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9LJ57|A0A1I9LJ57_9CAUD Uncharacterized protein OS=Stx converting phage vB_EcoS_P27 OX=1792287 GN=vBEcoSP27_20 PE=4 SV=1
MM1 pKa = 7.54SNINKK6 pKa = 10.02YY7 pKa = 10.44IIDD10 pKa = 4.3YY11 pKa = 9.74DD12 pKa = 3.51WKK14 pKa = 10.79ASIEE18 pKa = 3.99IEE20 pKa = 3.8IDD22 pKa = 3.1HH23 pKa = 7.35DD24 pKa = 4.32VMTEE28 pKa = 3.99EE29 pKa = 5.57KK30 pKa = 10.35LHH32 pKa = 6.08QINNFWSDD40 pKa = 2.68SEE42 pKa = 4.22YY43 pKa = 11.07RR44 pKa = 11.84LNKK47 pKa = 9.52HH48 pKa = 5.94GSVLNAVLIMLAQHH62 pKa = 6.9ALLIAISSDD71 pKa = 2.92LNAYY75 pKa = 9.62GVVCEE80 pKa = 4.87FDD82 pKa = 3.48WNDD85 pKa = 3.26GNGQEE90 pKa = 5.08GWPSMDD96 pKa = 2.65GSEE99 pKa = 4.97GIRR102 pKa = 11.84ITDD105 pKa = 3.36IDD107 pKa = 3.59TSGIFNSDD115 pKa = 3.38DD116 pKa = 3.55MTIKK120 pKa = 10.64AAA122 pKa = 3.9

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9LJ59|A0A1I9LJ59_9CAUD Uncharacterized protein OS=Stx converting phage vB_EcoS_P27 OX=1792287 GN=vBEcoSP27_22 PE=4 SV=1
MM1 pKa = 7.26TRR3 pKa = 11.84RR4 pKa = 11.84TQFKK8 pKa = 9.33GNSRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.62RR18 pKa = 11.84LKK20 pKa = 11.17AKK22 pKa = 10.38ALANGVLARR31 pKa = 11.84EE32 pKa = 4.21EE33 pKa = 4.75AISSEE38 pKa = 4.23VLHH41 pKa = 6.83RR42 pKa = 11.84PTLSRR47 pKa = 11.84AQIQAKK53 pKa = 8.11GTHH56 pKa = 5.29EE57 pKa = 4.03TPEE60 pKa = 4.7RR61 pKa = 11.84IEE63 pKa = 3.89DD64 pKa = 3.57AKK66 pKa = 10.63PIKK69 pKa = 10.49FMAQDD74 pKa = 3.8VIWQQKK80 pKa = 6.5EE81 pKa = 3.56YY82 pKa = 10.85RR83 pKa = 11.84RR84 pKa = 11.84NLEE87 pKa = 3.51RR88 pKa = 11.84AAIVYY93 pKa = 10.58ANEE96 pKa = 4.32FGHH99 pKa = 6.02KK100 pKa = 9.59QPEE103 pKa = 4.48TGVCLPNVAIYY114 pKa = 10.18AAGYY118 pKa = 9.31RR119 pKa = 11.84KK120 pKa = 9.92SKK122 pKa = 10.61QLTARR127 pKa = 3.76

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

18371

37

2867

208.8

23.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.27 ± 0.424

1.176 ± 0.202

5.824 ± 0.193

6.831 ± 0.236

3.331 ± 0.187

7.223 ± 0.553

1.845 ± 0.171

5.231 ± 0.383

6.162 ± 0.219

7.572 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.782 ± 0.144

4.48 ± 0.199

4.311 ± 0.287

4.496 ± 0.292

6.657 ± 0.322

6.053 ± 0.212

5.672 ± 0.217

6.38 ± 0.247

1.617 ± 0.156

3.086 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski