Bacillus phage 031MP003

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saundersvirus; unclassified Saundersvirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T4P2|A0A5J6T4P2_9CAUD Uncharacterized protein OS=Bacillus phage 031MP003 OX=2601657 GN=46 PE=4 SV=1
MM1 pKa = 7.47IKK3 pKa = 9.14VTVISKK9 pKa = 8.62KK10 pKa = 7.4TGKK13 pKa = 9.7VKK15 pKa = 10.53RR16 pKa = 11.84GEE18 pKa = 3.91FSARR22 pKa = 11.84EE23 pKa = 3.86LLEE26 pKa = 4.37SNEE29 pKa = 4.12DD30 pKa = 3.95EE31 pKa = 4.97IVDD34 pKa = 4.0TLTQCDD40 pKa = 4.22CQPVGEE46 pKa = 4.5TYY48 pKa = 10.73VVEE51 pKa = 4.59CNCCEE56 pKa = 3.72EE57 pKa = 4.04WEE59 pKa = 4.38EE60 pKa = 3.91YY61 pKa = 10.7VLYY64 pKa = 10.93FEE66 pKa = 6.35DD67 pKa = 5.61GDD69 pKa = 3.96IEE71 pKa = 5.5GDD73 pKa = 3.53AEE75 pKa = 4.07

Molecular weight: 8.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T7K9|A0A5J6T7K9_9CAUD Uncharacterized protein OS=Bacillus phage 031MP003 OX=2601657 GN=10 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 9.54CSKK7 pKa = 10.27KK8 pKa = 8.48RR9 pKa = 11.84TRR11 pKa = 11.84WDD13 pKa = 3.32YY14 pKa = 11.8FDD16 pKa = 3.96FTWIGDD22 pKa = 4.48LINGIFRR29 pKa = 11.84IWWW32 pKa = 3.47

Molecular weight: 4.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

17529

32

1328

192.6

21.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.137 ± 0.46

0.628 ± 0.098

5.927 ± 0.279

8.42 ± 0.65

3.811 ± 0.145

6.983 ± 0.339

1.62 ± 0.139

6.6 ± 0.186

7.793 ± 0.47

7.542 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.835 ± 0.188

5.163 ± 0.284

3.024 ± 0.187

3.6 ± 0.216

5.015 ± 0.247

5.442 ± 0.291

6.578 ± 0.49

6.726 ± 0.242

1.249 ± 0.103

3.908 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski