Amino acid dipepetide frequency for Arthrobacter phage Giantsbane

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.695AlaAla: 8.695 ± 1.177
0.393AlaCys: 0.393 ± 0.166
4.207AlaAsp: 4.207 ± 0.507
5.049AlaGlu: 5.049 ± 0.642
2.693AlaPhe: 2.693 ± 0.354
6.507AlaGly: 6.507 ± 1.155
1.739AlaHis: 1.739 ± 0.365
5.385AlaIle: 5.385 ± 0.535
6.731AlaLys: 6.731 ± 0.795
7.573AlaLeu: 7.573 ± 0.969
3.478AlaMet: 3.478 ± 0.599
3.758AlaAsn: 3.758 ± 0.434
3.085AlaPro: 3.085 ± 0.372
3.141AlaGln: 3.141 ± 0.447
4.936AlaArg: 4.936 ± 0.534
4.375AlaSer: 4.375 ± 0.494
5.441AlaThr: 5.441 ± 0.518
6.002AlaVal: 6.002 ± 0.626
1.234AlaTrp: 1.234 ± 0.279
2.861AlaTyr: 2.861 ± 0.353
0.0AlaXaa: 0.0 ± 0.0
Cys
0.393CysAla: 0.393 ± 0.186
0.168CysCys: 0.168 ± 0.103
0.617CysAsp: 0.617 ± 0.215
0.393CysGlu: 0.393 ± 0.194
0.337CysPhe: 0.337 ± 0.139
0.841CysGly: 0.841 ± 0.248
0.28CysHis: 0.28 ± 0.124
0.449CysIle: 0.449 ± 0.2
0.224CysLys: 0.224 ± 0.137
0.449CysLeu: 0.449 ± 0.208
0.056CysMet: 0.056 ± 0.058
0.112CysAsn: 0.112 ± 0.074
0.224CysPro: 0.224 ± 0.115
0.28CysGln: 0.28 ± 0.161
0.393CysArg: 0.393 ± 0.169
0.449CysSer: 0.449 ± 0.161
0.168CysThr: 0.168 ± 0.092
0.393CysVal: 0.393 ± 0.166
0.056CysTrp: 0.056 ± 0.061
0.337CysTyr: 0.337 ± 0.19
0.0CysXaa: 0.0 ± 0.0
Asp
6.058AspAla: 6.058 ± 0.546
0.337AspCys: 0.337 ± 0.133
3.646AspAsp: 3.646 ± 0.471
5.105AspGlu: 5.105 ± 0.657
1.907AspPhe: 1.907 ± 0.396
4.936AspGly: 4.936 ± 0.508
0.841AspHis: 0.841 ± 0.253
4.6AspIle: 4.6 ± 0.528
2.973AspLys: 2.973 ± 0.376
5.497AspLeu: 5.497 ± 0.489
1.739AspMet: 1.739 ± 0.282
2.917AspAsn: 2.917 ± 0.409
2.412AspPro: 2.412 ± 0.394
2.356AspGln: 2.356 ± 0.385
2.749AspArg: 2.749 ± 0.446
2.749AspSer: 2.749 ± 0.342
3.534AspThr: 3.534 ± 0.497
3.702AspVal: 3.702 ± 0.632
1.122AspTrp: 1.122 ± 0.256
1.851AspTyr: 1.851 ± 0.293
0.0AspXaa: 0.0 ± 0.0
Glu
5.161GluAla: 5.161 ± 0.629
0.729GluCys: 0.729 ± 0.242
3.871GluAsp: 3.871 ± 0.581
4.768GluGlu: 4.768 ± 0.785
2.468GluPhe: 2.468 ± 0.415
4.263GluGly: 4.263 ± 0.504
1.683GluHis: 1.683 ± 0.326
3.422GluIle: 3.422 ± 0.382
3.702GluLys: 3.702 ± 0.498
5.329GluLeu: 5.329 ± 0.519
2.356GluMet: 2.356 ± 0.361
2.468GluAsn: 2.468 ± 0.405
2.805GluPro: 2.805 ± 0.548
2.58GluGln: 2.58 ± 0.341
3.59GluArg: 3.59 ± 0.407
2.749GluSer: 2.749 ± 0.449
4.712GluThr: 4.712 ± 0.615
5.049GluVal: 5.049 ± 0.779
1.402GluTrp: 1.402 ± 0.274
1.739GluTyr: 1.739 ± 0.308
0.0GluXaa: 0.0 ± 0.0
Phe
3.253PheAla: 3.253 ± 0.497
0.393PheCys: 0.393 ± 0.168
2.749PheAsp: 2.749 ± 0.419
2.749PheGlu: 2.749 ± 0.366
1.066PhePhe: 1.066 ± 0.246
2.861PheGly: 2.861 ± 0.499
0.673PheHis: 0.673 ± 0.194
2.244PheIle: 2.244 ± 0.28
2.019PheLys: 2.019 ± 0.389
2.917PheLeu: 2.917 ± 0.434
1.01PheMet: 1.01 ± 0.27
2.356PheAsn: 2.356 ± 0.302
1.178PhePro: 1.178 ± 0.208
1.29PheGln: 1.29 ± 0.246
1.851PheArg: 1.851 ± 0.386
2.244PheSer: 2.244 ± 0.367
2.805PheThr: 2.805 ± 0.373
2.076PheVal: 2.076 ± 0.348
0.505PheTrp: 0.505 ± 0.162
1.066PheTyr: 1.066 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
7.124GlyAla: 7.124 ± 0.787
0.673GlyCys: 0.673 ± 0.297
5.273GlyAsp: 5.273 ± 0.561
4.431GlyGlu: 4.431 ± 0.444
3.478GlyPhe: 3.478 ± 0.54
6.058GlyGly: 6.058 ± 1.645
1.346GlyHis: 1.346 ± 0.282
4.768GlyIle: 4.768 ± 0.815
4.656GlyLys: 4.656 ± 0.537
5.778GlyLeu: 5.778 ± 0.964
2.917GlyMet: 2.917 ± 0.809
2.636GlyAsn: 2.636 ± 0.369
1.627GlyPro: 1.627 ± 0.324
1.683GlyGln: 1.683 ± 0.367
2.412GlyArg: 2.412 ± 0.411
5.161GlySer: 5.161 ± 0.628
5.778GlyThr: 5.778 ± 0.684
5.722GlyVal: 5.722 ± 0.525
1.066GlyTrp: 1.066 ± 0.252
2.805GlyTyr: 2.805 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
1.402HisAla: 1.402 ± 0.488
0.337HisCys: 0.337 ± 0.142
0.841HisAsp: 0.841 ± 0.201
1.346HisGlu: 1.346 ± 0.308
0.561HisPhe: 0.561 ± 0.19
0.954HisGly: 0.954 ± 0.225
0.449HisHis: 0.449 ± 0.134
1.402HisIle: 1.402 ± 0.272
1.627HisLys: 1.627 ± 0.316
1.402HisLeu: 1.402 ± 0.29
0.505HisMet: 0.505 ± 0.18
0.785HisAsn: 0.785 ± 0.269
1.066HisPro: 1.066 ± 0.23
1.066HisGln: 1.066 ± 0.237
0.785HisArg: 0.785 ± 0.234
1.01HisSer: 1.01 ± 0.258
0.785HisThr: 0.785 ± 0.243
1.402HisVal: 1.402 ± 0.329
0.224HisTrp: 0.224 ± 0.129
1.29HisTyr: 1.29 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
5.722IleAla: 5.722 ± 0.837
0.28IleCys: 0.28 ± 0.137
4.544IleAsp: 4.544 ± 0.411
3.141IleGlu: 3.141 ± 0.496
2.412IlePhe: 2.412 ± 0.401
3.59IleGly: 3.59 ± 0.763
1.178IleHis: 1.178 ± 0.29
3.085IleIle: 3.085 ± 0.46
3.927IleLys: 3.927 ± 0.522
5.105IleLeu: 5.105 ± 0.56
1.346IleMet: 1.346 ± 0.276
3.534IleAsn: 3.534 ± 0.406
2.749IlePro: 2.749 ± 0.469
1.851IleGln: 1.851 ± 0.333
2.917IleArg: 2.917 ± 0.384
3.534IleSer: 3.534 ± 0.438
4.039IleThr: 4.039 ± 0.529
4.488IleVal: 4.488 ± 0.516
0.729IleTrp: 0.729 ± 0.17
2.356IleTyr: 2.356 ± 0.354
0.0IleXaa: 0.0 ± 0.0
Lys
6.395LysAla: 6.395 ± 0.851
0.449LysCys: 0.449 ± 0.142
3.478LysAsp: 3.478 ± 0.69
5.217LysGlu: 5.217 ± 0.674
2.356LysPhe: 2.356 ± 0.288
5.217LysGly: 5.217 ± 0.635
1.795LysHis: 1.795 ± 0.386
3.758LysIle: 3.758 ± 0.558
5.722LysLys: 5.722 ± 0.78
5.609LysLeu: 5.609 ± 0.612
2.412LysMet: 2.412 ± 0.421
2.917LysAsn: 2.917 ± 0.498
3.478LysPro: 3.478 ± 0.653
2.3LysGln: 2.3 ± 0.318
3.758LysArg: 3.758 ± 0.47
4.263LysSer: 4.263 ± 0.703
3.422LysThr: 3.422 ± 0.372
4.039LysVal: 4.039 ± 0.498
1.066LysTrp: 1.066 ± 0.251
1.795LysTyr: 1.795 ± 0.342
0.0LysXaa: 0.0 ± 0.0
Leu
6.507LeuAla: 6.507 ± 0.71
0.393LeuCys: 0.393 ± 0.154
4.936LeuAsp: 4.936 ± 0.441
5.161LeuGlu: 5.161 ± 0.594
3.085LeuPhe: 3.085 ± 0.445
5.89LeuGly: 5.89 ± 1.121
1.402LeuHis: 1.402 ± 0.35
4.992LeuIle: 4.992 ± 0.659
5.89LeuLys: 5.89 ± 0.811
4.88LeuLeu: 4.88 ± 0.676
1.907LeuMet: 1.907 ± 0.378
3.197LeuAsn: 3.197 ± 0.373
3.253LeuPro: 3.253 ± 0.475
2.58LeuGln: 2.58 ± 0.319
4.095LeuArg: 4.095 ± 0.497
5.273LeuSer: 5.273 ± 0.708
5.553LeuThr: 5.553 ± 0.617
6.283LeuVal: 6.283 ± 0.724
0.841LeuTrp: 0.841 ± 0.231
2.861LeuTyr: 2.861 ± 0.454
0.0LeuXaa: 0.0 ± 0.0
Met
3.253MetAla: 3.253 ± 0.464
0.337MetCys: 0.337 ± 0.163
1.515MetAsp: 1.515 ± 0.308
1.739MetGlu: 1.739 ± 0.349
1.122MetPhe: 1.122 ± 0.221
2.019MetGly: 2.019 ± 0.612
0.561MetHis: 0.561 ± 0.174
1.851MetIle: 1.851 ± 0.405
1.907MetLys: 1.907 ± 0.323
2.468MetLeu: 2.468 ± 0.448
0.954MetMet: 0.954 ± 0.343
0.954MetAsn: 0.954 ± 0.244
0.841MetPro: 0.841 ± 0.211
1.01MetGln: 1.01 ± 0.33
0.898MetArg: 0.898 ± 0.232
3.422MetSer: 3.422 ± 0.44
1.851MetThr: 1.851 ± 0.302
1.963MetVal: 1.963 ± 0.336
0.28MetTrp: 0.28 ± 0.139
0.561MetTyr: 0.561 ± 0.221
0.0MetXaa: 0.0 ± 0.0
Asn
3.871AsnAla: 3.871 ± 0.433
0.337AsnCys: 0.337 ± 0.15
1.963AsnAsp: 1.963 ± 0.366
3.085AsnGlu: 3.085 ± 0.409
1.458AsnPhe: 1.458 ± 0.281
4.319AsnGly: 4.319 ± 0.569
0.729AsnHis: 0.729 ± 0.19
2.3AsnIle: 2.3 ± 0.416
2.58AsnLys: 2.58 ± 0.339
3.253AsnLeu: 3.253 ± 0.531
1.515AsnMet: 1.515 ± 0.276
1.851AsnAsn: 1.851 ± 0.36
2.412AsnPro: 2.412 ± 0.401
2.132AsnGln: 2.132 ± 0.449
2.636AsnArg: 2.636 ± 0.478
2.973AsnSer: 2.973 ± 0.35
2.412AsnThr: 2.412 ± 0.3
2.356AsnVal: 2.356 ± 0.335
0.673AsnTrp: 0.673 ± 0.218
1.739AsnTyr: 1.739 ± 0.336
0.0AsnXaa: 0.0 ± 0.0
Pro
3.534ProAla: 3.534 ± 0.472
0.393ProCys: 0.393 ± 0.187
2.805ProAsp: 2.805 ± 0.433
3.366ProGlu: 3.366 ± 0.539
1.29ProPhe: 1.29 ± 0.254
3.646ProGly: 3.646 ± 0.51
0.617ProHis: 0.617 ± 0.185
2.244ProIle: 2.244 ± 0.318
3.029ProLys: 3.029 ± 0.582
2.3ProLeu: 2.3 ± 0.367
1.178ProMet: 1.178 ± 0.297
1.963ProAsn: 1.963 ± 0.413
1.571ProPro: 1.571 ± 0.346
0.785ProGln: 0.785 ± 0.219
2.132ProArg: 2.132 ± 0.339
2.861ProSer: 2.861 ± 0.347
2.805ProThr: 2.805 ± 0.41
2.805ProVal: 2.805 ± 0.311
0.393ProTrp: 0.393 ± 0.153
0.898ProTyr: 0.898 ± 0.251
0.0ProXaa: 0.0 ± 0.0
Gln
2.636GlnAla: 2.636 ± 0.429
0.28GlnCys: 0.28 ± 0.138
1.963GlnAsp: 1.963 ± 0.34
1.907GlnGlu: 1.907 ± 0.336
1.402GlnPhe: 1.402 ± 0.3
2.412GlnGly: 2.412 ± 0.292
0.785GlnHis: 0.785 ± 0.258
2.019GlnIle: 2.019 ± 0.345
2.749GlnLys: 2.749 ± 0.449
2.805GlnLeu: 2.805 ± 0.444
1.178GlnMet: 1.178 ± 0.279
1.29GlnAsn: 1.29 ± 0.234
1.178GlnPro: 1.178 ± 0.258
1.683GlnGln: 1.683 ± 0.29
2.188GlnArg: 2.188 ± 0.364
1.458GlnSer: 1.458 ± 0.269
2.132GlnThr: 2.132 ± 0.391
2.076GlnVal: 2.076 ± 0.334
0.729GlnTrp: 0.729 ± 0.188
1.571GlnTyr: 1.571 ± 0.299
0.0GlnXaa: 0.0 ± 0.0
Arg
2.805ArgAla: 2.805 ± 0.445
0.28ArgCys: 0.28 ± 0.117
2.805ArgAsp: 2.805 ± 0.42
3.758ArgGlu: 3.758 ± 0.463
1.066ArgPhe: 1.066 ± 0.213
2.188ArgGly: 2.188 ± 0.291
1.01ArgHis: 1.01 ± 0.238
3.197ArgIle: 3.197 ± 0.439
4.431ArgLys: 4.431 ± 0.628
4.768ArgLeu: 4.768 ± 0.52
1.234ArgMet: 1.234 ± 0.239
2.805ArgAsn: 2.805 ± 0.331
1.739ArgPro: 1.739 ± 0.328
2.188ArgGln: 2.188 ± 0.459
3.478ArgArg: 3.478 ± 0.699
2.805ArgSer: 2.805 ± 0.312
2.917ArgThr: 2.917 ± 0.431
3.758ArgVal: 3.758 ± 0.424
0.673ArgTrp: 0.673 ± 0.197
2.244ArgTyr: 2.244 ± 0.374
0.0ArgXaa: 0.0 ± 0.0
Ser
5.049SerAla: 5.049 ± 0.538
0.168SerCys: 0.168 ± 0.101
3.871SerAsp: 3.871 ± 0.48
3.422SerGlu: 3.422 ± 0.391
2.58SerPhe: 2.58 ± 0.359
5.834SerGly: 5.834 ± 0.616
1.01SerHis: 1.01 ± 0.24
3.814SerIle: 3.814 ± 0.405
4.431SerLys: 4.431 ± 0.636
4.6SerLeu: 4.6 ± 0.462
1.458SerMet: 1.458 ± 0.222
3.029SerAsn: 3.029 ± 0.414
3.029SerPro: 3.029 ± 0.387
1.795SerGln: 1.795 ± 0.374
2.636SerArg: 2.636 ± 0.32
4.6SerSer: 4.6 ± 0.671
3.702SerThr: 3.702 ± 0.513
2.861SerVal: 2.861 ± 0.388
1.066SerTrp: 1.066 ± 0.252
1.683SerTyr: 1.683 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
5.385ThrAla: 5.385 ± 0.628
0.112ThrCys: 0.112 ± 0.082
3.927ThrAsp: 3.927 ± 0.506
3.646ThrGlu: 3.646 ± 0.629
3.085ThrPhe: 3.085 ± 0.412
5.385ThrGly: 5.385 ± 0.628
0.841ThrHis: 0.841 ± 0.249
4.095ThrIle: 4.095 ± 0.57
3.983ThrLys: 3.983 ± 0.458
5.722ThrLeu: 5.722 ± 0.514
1.234ThrMet: 1.234 ± 0.22
2.749ThrAsn: 2.749 ± 0.416
3.927ThrPro: 3.927 ± 0.508
1.683ThrGln: 1.683 ± 0.301
2.58ThrArg: 2.58 ± 0.298
3.253ThrSer: 3.253 ± 0.422
4.263ThrThr: 4.263 ± 0.507
4.095ThrVal: 4.095 ± 0.545
0.898ThrTrp: 0.898 ± 0.273
2.244ThrTyr: 2.244 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
6.283ValAla: 6.283 ± 0.577
0.224ValCys: 0.224 ± 0.118
3.758ValAsp: 3.758 ± 0.471
4.039ValGlu: 4.039 ± 0.598
2.524ValPhe: 2.524 ± 0.42
4.207ValGly: 4.207 ± 0.6
1.122ValHis: 1.122 ± 0.2
4.6ValIle: 4.6 ± 0.538
5.722ValLys: 5.722 ± 0.532
5.105ValLeu: 5.105 ± 0.604
1.795ValMet: 1.795 ± 0.367
3.253ValAsn: 3.253 ± 0.407
2.524ValPro: 2.524 ± 0.424
2.076ValGln: 2.076 ± 0.378
3.871ValArg: 3.871 ± 0.557
4.375ValSer: 4.375 ± 0.479
3.871ValThr: 3.871 ± 0.54
5.105ValVal: 5.105 ± 0.556
0.729ValTrp: 0.729 ± 0.196
2.356ValTyr: 2.356 ± 0.429
0.0ValXaa: 0.0 ± 0.0
Trp
0.785TrpAla: 0.785 ± 0.259
0.056TrpCys: 0.056 ± 0.054
1.627TrpAsp: 1.627 ± 0.312
0.841TrpGlu: 0.841 ± 0.203
0.561TrpPhe: 0.561 ± 0.18
1.29TrpGly: 1.29 ± 0.275
0.337TrpHis: 0.337 ± 0.178
0.561TrpIle: 0.561 ± 0.194
0.729TrpLys: 0.729 ± 0.188
1.066TrpLeu: 1.066 ± 0.203
0.337TrpMet: 0.337 ± 0.133
0.561TrpAsn: 0.561 ± 0.224
0.168TrpPro: 0.168 ± 0.102
0.785TrpGln: 0.785 ± 0.229
0.729TrpArg: 0.729 ± 0.232
0.841TrpSer: 0.841 ± 0.239
1.346TrpThr: 1.346 ± 0.251
1.01TrpVal: 1.01 ± 0.275
0.224TrpTrp: 0.224 ± 0.123
0.617TrpTyr: 0.617 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.973TyrAla: 2.973 ± 0.392
0.28TyrCys: 0.28 ± 0.153
2.636TyrAsp: 2.636 ± 0.473
1.739TyrGlu: 1.739 ± 0.359
1.851TyrPhe: 1.851 ± 0.336
2.973TyrGly: 2.973 ± 0.417
0.841TyrHis: 0.841 ± 0.293
1.739TyrIle: 1.739 ± 0.316
2.412TyrLys: 2.412 ± 0.316
2.356TyrLeu: 2.356 ± 0.286
0.841TyrMet: 0.841 ± 0.213
1.458TyrAsn: 1.458 ± 0.193
1.346TyrPro: 1.346 ± 0.318
1.178TyrGln: 1.178 ± 0.258
1.515TyrArg: 1.515 ± 0.296
2.244TyrSer: 2.244 ± 0.328
1.571TyrThr: 1.571 ± 0.315
2.356TyrVal: 2.356 ± 0.419
0.561TyrTrp: 0.561 ± 0.22
1.402TyrTyr: 1.402 ± 0.354
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (17828 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski