Bacteroides uniformis CAG:3

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7ELP5|R7ELP5_9BACE Uncharacterized protein OS=Bacteroides uniformis CAG:3 OX=1263055 GN=BN594_03137 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.42MFKK5 pKa = 10.12MMAASMLGVALCLGFTACSDD25 pKa = 3.65DD26 pKa = 4.64DD27 pKa = 4.19EE28 pKa = 5.89NEE30 pKa = 4.03NGEE33 pKa = 4.23GGEE36 pKa = 4.17NTATVVNPSQVFTGGLPKK54 pKa = 10.24SVSGMAISHH63 pKa = 5.76NEE65 pKa = 3.67EE66 pKa = 3.83GLVTNITTEE75 pKa = 4.47DD76 pKa = 3.28GDD78 pKa = 4.01KK79 pKa = 11.19AVFEE83 pKa = 4.47YY84 pKa = 10.84FPATTKK90 pKa = 11.14ADD92 pKa = 3.59VAKK95 pKa = 10.44DD96 pKa = 3.37RR97 pKa = 11.84ARR99 pKa = 11.84ITVTDD104 pKa = 3.57EE105 pKa = 4.11EE106 pKa = 5.85GDD108 pKa = 3.78VTEE111 pKa = 6.43LNLQLNSDD119 pKa = 4.49GYY121 pKa = 11.63VEE123 pKa = 4.55FCNSIDD129 pKa = 4.12HH130 pKa = 7.04AGTPDD135 pKa = 3.45ADD137 pKa = 3.57EE138 pKa = 4.77FTWEE142 pKa = 4.13MEE144 pKa = 3.98YY145 pKa = 8.99DD146 pKa = 3.89TEE148 pKa = 4.14GHH150 pKa = 5.9LVVMKK155 pKa = 10.47RR156 pKa = 11.84SEE158 pKa = 4.23SDD160 pKa = 3.69GEE162 pKa = 4.25ITNITYY168 pKa = 10.65KK169 pKa = 10.94DD170 pKa = 3.27GDD172 pKa = 3.97VVKK175 pKa = 10.15TSTRR179 pKa = 11.84YY180 pKa = 9.26VASGDD185 pKa = 3.82LNGDD189 pKa = 4.18GIIDD193 pKa = 4.59SNDD196 pKa = 2.66EE197 pKa = 4.02WEE199 pKa = 4.23YY200 pKa = 11.45SAAIDD205 pKa = 3.85YY206 pKa = 7.7TTDD209 pKa = 3.53NITAPIEE216 pKa = 4.16NKK218 pKa = 9.95GCLMLFDD225 pKa = 5.86EE226 pKa = 5.11ILDD229 pKa = 3.59VDD231 pKa = 4.43MDD233 pKa = 3.88EE234 pKa = 5.25MIYY237 pKa = 10.46AYY239 pKa = 10.42YY240 pKa = 11.07GGMLGKK246 pKa = 8.84ATKK249 pKa = 9.95HH250 pKa = 5.63LPLVGHH256 pKa = 5.31YY257 pKa = 8.47TYY259 pKa = 11.0NGEE262 pKa = 4.28DD263 pKa = 3.85SVSDD267 pKa = 3.74MYY269 pKa = 9.7FTWTLNSDD277 pKa = 4.1SYY279 pKa = 10.13PTEE282 pKa = 4.38LVVKK286 pKa = 8.81DD287 pKa = 3.09QWDD290 pKa = 3.81EE291 pKa = 3.98YY292 pKa = 11.04RR293 pKa = 11.84CTFTWW298 pKa = 3.12

Molecular weight: 33.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7EGQ5|R7EGQ5_9BACE Uncharacterized protein OS=Bacteroides uniformis CAG:3 OX=1263055 GN=BN594_02515 PE=4 SV=1
MM1 pKa = 7.63DD2 pKa = 4.52HH3 pKa = 6.94AAVIRR8 pKa = 11.84RR9 pKa = 11.84TLFGRR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84QLRR20 pKa = 11.84IFGGRR25 pKa = 11.84HH26 pKa = 4.13KK27 pKa = 10.84FKK29 pKa = 10.21IYY31 pKa = 10.55RR32 pKa = 11.84MAVAGSLIHH41 pKa = 6.41LHH43 pKa = 5.23NQFMVLTEE51 pKa = 4.04RR52 pKa = 11.84QFGIAEE58 pKa = 4.1FSVFGSPYY66 pKa = 9.75QHH68 pKa = 5.69VTLTGRR74 pKa = 11.84QKK76 pKa = 10.98SLSLCIGLLPGYY88 pKa = 9.81SIEE91 pKa = 3.9LRR93 pKa = 11.84IIIEE97 pKa = 4.39LEE99 pKa = 3.66LHH101 pKa = 5.19TSIRR105 pKa = 11.84HH106 pKa = 5.6RR107 pKa = 11.84ISMSVHH113 pKa = 6.33DD114 pKa = 5.03RR115 pKa = 11.84NCGLSRR121 pKa = 11.84RR122 pKa = 11.84CIVTDD127 pKa = 3.87YY128 pKa = 11.1IDD130 pKa = 3.77FRR132 pKa = 11.84ISRR135 pKa = 11.84SDD137 pKa = 2.74RR138 pKa = 11.84HH139 pKa = 5.61HH140 pKa = 6.84FLRR143 pKa = 11.84SFITSEE149 pKa = 3.9DD150 pKa = 3.52LGMHH154 pKa = 5.28QHH156 pKa = 6.04TATGRR161 pKa = 11.84SIEE164 pKa = 4.07PTKK167 pKa = 10.62VEE169 pKa = 3.97YY170 pKa = 10.72RR171 pKa = 11.84FGFAGSKK178 pKa = 10.02KK179 pKa = 10.07IPFPVCPCFYY189 pKa = 10.32PRR191 pKa = 11.84MIVVGMRR198 pKa = 11.84PPGCVDD204 pKa = 3.22LTGRR208 pKa = 11.84DD209 pKa = 3.31THH211 pKa = 6.08RR212 pKa = 11.84TQGGNQQGRR221 pKa = 11.84FLATTPVCRR230 pKa = 11.84AYY232 pKa = 10.38RR233 pKa = 11.84CQRR236 pKa = 11.84RR237 pKa = 11.84TGTGIGGSIDD247 pKa = 3.83SLLVAPVVHH256 pKa = 6.49LQNGIIEE263 pKa = 4.36GQRR266 pKa = 11.84LDD268 pKa = 3.87TILQFFVKK276 pKa = 10.07HH277 pKa = 5.19YY278 pKa = 8.63PCTVQMLIVDD288 pKa = 3.71PHH290 pKa = 6.98RR291 pKa = 11.84KK292 pKa = 9.61YY293 pKa = 11.49KK294 pKa = 7.73MTKK297 pKa = 9.59QILRR301 pKa = 11.84NNFSPRR307 pKa = 11.84HH308 pKa = 5.59LIGSLLGGSHH318 pKa = 5.59IHH320 pKa = 5.49QMKK323 pKa = 10.07FSGIIGNVCQRR334 pKa = 11.84HH335 pKa = 5.27IGIKK339 pKa = 9.49EE340 pKa = 3.89LQSFAFVRR348 pKa = 11.84RR349 pKa = 11.84QVHH352 pKa = 5.6IEE354 pKa = 3.83YY355 pKa = 10.19GKK357 pKa = 10.6QIALRR362 pKa = 11.84QPFFFLIEE370 pKa = 4.24VLLHH374 pKa = 5.55TLTVRR379 pKa = 11.84SVCNEE384 pKa = 3.51RR385 pKa = 11.84TFAARR390 pKa = 11.84GKK392 pKa = 8.11QTYY395 pKa = 8.76QSEE398 pKa = 4.54KK399 pKa = 9.95SQKK402 pKa = 9.85SCNSFHH408 pKa = 6.3NAIIKK413 pKa = 9.4VEE415 pKa = 3.98PQKK418 pKa = 11.41

Molecular weight: 47.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3570

0

3570

1263016

29

1965

353.8

39.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.378 ± 0.043

1.29 ± 0.017

5.47 ± 0.032

6.578 ± 0.042

4.568 ± 0.028

6.997 ± 0.036

1.956 ± 0.016

6.639 ± 0.034

6.362 ± 0.037

9.185 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.017

4.929 ± 0.041

3.796 ± 0.023

3.452 ± 0.019

4.757 ± 0.03

5.972 ± 0.034

5.592 ± 0.032

6.528 ± 0.032

1.296 ± 0.016

4.445 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski