Grouper iridovirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Singapore grouper iridovirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5GAF6|Q5GAF6_9VIRU Uncharacterized protein OS=Grouper iridovirus OX=127569 GN=GIV60 PE=4 SV=1
MM1 pKa = 6.92QRR3 pKa = 11.84SINLLAVVMLTGVLCSNVSIVDD25 pKa = 3.43EE26 pKa = 5.21FGTDD30 pKa = 4.04DD31 pKa = 5.47YY32 pKa = 11.57IDD34 pKa = 4.78EE35 pKa = 4.92NDD37 pKa = 3.93ACPPDD42 pKa = 3.97TPAEE46 pKa = 4.31EE47 pKa = 4.08ICDD50 pKa = 3.66VDD52 pKa = 4.09EE53 pKa = 4.87EE54 pKa = 5.02SCGACNITLIPTVSPIEE71 pKa = 4.15TPEE74 pKa = 4.01PEE76 pKa = 4.45GNGTSTEE83 pKa = 4.23SSTPPPKK90 pKa = 10.39PEE92 pKa = 3.84VPEE95 pKa = 4.04PEE97 pKa = 4.35GNGTSTEE104 pKa = 4.23SSTPPPKK111 pKa = 10.39PEE113 pKa = 3.84VPEE116 pKa = 4.04PEE118 pKa = 4.35GNGTSTEE125 pKa = 4.23SSTPPPKK132 pKa = 10.39PEE134 pKa = 3.81VPEE137 pKa = 4.33PEE139 pKa = 4.05GKK141 pKa = 8.94TIIEE145 pKa = 4.35VNTTSEE151 pKa = 3.91NDD153 pKa = 3.18FKK155 pKa = 11.05QIEE158 pKa = 4.97DD159 pKa = 3.29IHH161 pKa = 5.47PTKK164 pKa = 10.94DD165 pKa = 3.03GGASNGLSPGILALTTVGSIMLVSAVAAGIVYY197 pKa = 10.24AVQNKK202 pKa = 7.9PAQSAMM208 pKa = 3.62

Molecular weight: 21.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5GAL0|Q5GAL0_9VIRU Uncharacterized protein OS=Grouper iridovirus OX=127569 GN=GIV06 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 10.58SNRR5 pKa = 11.84PCLMGCNGHH14 pKa = 7.29AMVAQPAQPACPLTAKK30 pKa = 10.27KK31 pKa = 10.06PSRR34 pKa = 11.84CPSGCVKK41 pKa = 10.71SPAKK45 pKa = 10.15RR46 pKa = 11.84KK47 pKa = 10.24SPTSRR52 pKa = 11.84KK53 pKa = 9.4SPARR57 pKa = 11.84RR58 pKa = 11.84KK59 pKa = 10.31SPTSRR64 pKa = 11.84KK65 pKa = 9.4SPARR69 pKa = 11.84RR70 pKa = 11.84KK71 pKa = 10.31SPTSRR76 pKa = 11.84KK77 pKa = 9.4SPARR81 pKa = 11.84RR82 pKa = 11.84KK83 pKa = 10.31SPTSRR88 pKa = 11.84KK89 pKa = 9.4SPARR93 pKa = 11.84RR94 pKa = 11.84KK95 pKa = 10.31SPTSRR100 pKa = 11.84KK101 pKa = 9.4SPARR105 pKa = 11.84RR106 pKa = 11.84KK107 pKa = 10.31SPTSRR112 pKa = 11.84KK113 pKa = 9.4SPARR117 pKa = 11.84RR118 pKa = 11.84KK119 pKa = 10.31SPTSRR124 pKa = 11.84KK125 pKa = 8.99SPARR129 pKa = 11.84GRR131 pKa = 11.84SPTRR135 pKa = 11.84RR136 pKa = 11.84KK137 pKa = 8.38TPTTPSKK144 pKa = 11.04CPTSYY149 pKa = 10.22PSSMKK154 pKa = 10.21SGSSRR159 pKa = 11.84RR160 pKa = 11.84SKK162 pKa = 10.77SSSASKK168 pKa = 10.8RR169 pKa = 11.84EE170 pKa = 3.88VCFMAAARR178 pKa = 4.18

Molecular weight: 19.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

35180

62

1268

359.0

39.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.408 ± 0.504

2.297 ± 0.18

5.503 ± 0.144

6.362 ± 0.347

3.459 ± 0.158

6.18 ± 0.412

1.998 ± 0.107

4.656 ± 0.166

6.583 ± 0.229

8.223 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.099

3.962 ± 0.142

5.941 ± 0.392

3.192 ± 0.133

5.711 ± 0.31

6.623 ± 0.252

6.393 ± 0.154

7.075 ± 0.24

1.333 ± 0.101

3.329 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski