Streptosporangium subroseum
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9643 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239EI34|A0A239EI34_9ACTN Uncharacterized protein OS=Streptosporangium subroseum OX=106412 GN=SAMN05216276_1009162 PE=4 SV=1
MM1 pKa = 7.26 FAYY4 pKa = 10.38 DD5 pKa = 3.42 NGGHH9 pKa = 6.23 FSCHH13 pKa = 5.54 EE14 pKa = 4.37 ADD16 pKa = 4.28 SVAALLMVAGHH27 pKa = 6.89 GDD29 pKa = 3.49 AAITWLEE36 pKa = 3.73 GHH38 pKa = 6.99 AEE40 pKa = 3.98 ADD42 pKa = 3.8 SEE44 pKa = 4.43 GSDD47 pKa = 2.99 IHH49 pKa = 9.02 IEE51 pKa = 3.94 FDD53 pKa = 3.94 DD54 pKa = 4.91 DD55 pKa = 4.83 DD56 pKa = 4.01 VFKK59 pKa = 11.15 AVDD62 pKa = 3.03 IPAYY66 pKa = 10.4 VDD68 pKa = 3.53 SLLSS72 pKa = 3.42
Molecular weight: 7.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 0.846
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.811
Protein with the highest isoelectric point:
>tr|A0A239NZL9|A0A239NZL9_9ACTN Uncharacterized protein OS=Streptosporangium subroseum OX=106412 GN=SAMN05216276_10772 PE=4 SV=1
MM1 pKa = 7.51 TKK3 pKa = 9.09 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 10.21 GRR40 pKa = 11.84 VEE42 pKa = 4.17 LSAA45 pKa = 5.19
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9643
0
9643
3066309
25
7891
318.0
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.677 ± 0.039
0.749 ± 0.007
5.793 ± 0.018
5.637 ± 0.025
2.911 ± 0.015
9.291 ± 0.025
2.175 ± 0.014
3.956 ± 0.019
2.153 ± 0.02
10.389 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.937 ± 0.011
2.012 ± 0.018
5.945 ± 0.021
2.771 ± 0.015
7.816 ± 0.03
5.473 ± 0.018
6.115 ± 0.032
8.521 ± 0.022
1.546 ± 0.01
2.135 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here