Lake Sarah-associated circular virus-16

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA49|A0A126GA49_9VIRU Coat protein OS=Lake Sarah-associated circular virus-16 OX=1685742 PE=4 SV=1
MM1 pKa = 7.7ANYY4 pKa = 9.44TDD6 pKa = 4.29ADD8 pKa = 3.57EE9 pKa = 4.83HH10 pKa = 6.69HH11 pKa = 6.89FLNTRR16 pKa = 11.84TKK18 pKa = 9.27AVHH21 pKa = 5.29FVSYY25 pKa = 7.65EE26 pKa = 3.98TSNEE30 pKa = 4.07TVEE33 pKa = 4.63RR34 pKa = 11.84LASLKK39 pKa = 9.25TDD41 pKa = 3.1YY42 pKa = 10.7MIIGDD47 pKa = 4.17EE48 pKa = 4.25HH49 pKa = 7.22CPTTGRR55 pKa = 11.84HH56 pKa = 5.74HH57 pKa = 6.47LQCYY61 pKa = 10.06LYY63 pKa = 8.99TKK65 pKa = 10.89NKK67 pKa = 7.78ITLSTIHH74 pKa = 6.78KK75 pKa = 10.34ACLKK79 pKa = 9.68KK80 pKa = 10.77CKK82 pKa = 10.29LFIAKK87 pKa = 8.02GTAEE91 pKa = 4.05QNQTYY96 pKa = 9.99CSKK99 pKa = 11.06EE100 pKa = 3.83KK101 pKa = 10.3ILYY104 pKa = 9.9EE105 pKa = 4.5SGTLPSGQGKK115 pKa = 7.84RR116 pKa = 11.84TDD118 pKa = 3.86LDD120 pKa = 3.49TTRR123 pKa = 11.84ANLLAGGTLRR133 pKa = 11.84DD134 pKa = 3.79VVLEE138 pKa = 3.96ATSYY142 pKa = 11.02QSVKK146 pKa = 9.9MAEE149 pKa = 4.24QILKK153 pKa = 8.87YY154 pKa = 10.59HH155 pKa = 6.22EE156 pKa = 4.68IKK158 pKa = 10.83RR159 pKa = 11.84NWKK162 pKa = 9.68PIVKK166 pKa = 8.73WFWGATGTGKK176 pKa = 10.58SLTAYY181 pKa = 10.54DD182 pKa = 4.92EE183 pKa = 4.3LDD185 pKa = 3.59KK186 pKa = 11.57DD187 pKa = 3.9DD188 pKa = 5.06TYY190 pKa = 11.73TAMSTGKK197 pKa = 8.6WWDD200 pKa = 3.61GYY202 pKa = 10.26DD203 pKa = 3.16AHH205 pKa = 7.43SCVVIDD211 pKa = 4.41DD212 pKa = 3.87MRR214 pKa = 11.84RR215 pKa = 11.84DD216 pKa = 3.4FCKK219 pKa = 9.89FHH221 pKa = 6.68EE222 pKa = 4.4LLRR225 pKa = 11.84YY226 pKa = 9.4LDD228 pKa = 4.34RR229 pKa = 11.84YY230 pKa = 7.4PTIVEE235 pKa = 4.4TKK237 pKa = 9.57GGTRR241 pKa = 11.84QFLAKK246 pKa = 10.19QIIITSCYY254 pKa = 9.96APEE257 pKa = 4.4DD258 pKa = 3.69MYY260 pKa = 10.55EE261 pKa = 4.0TRR263 pKa = 11.84EE264 pKa = 4.96DD265 pKa = 3.27IQQLLRR271 pKa = 11.84RR272 pKa = 11.84IDD274 pKa = 3.57EE275 pKa = 4.12VRR277 pKa = 11.84EE278 pKa = 4.04FTAEE282 pKa = 3.92SLGKK286 pKa = 10.19INSINN291 pKa = 3.21

Molecular weight: 33.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA49|A0A126GA49_9VIRU Coat protein OS=Lake Sarah-associated circular virus-16 OX=1685742 PE=4 SV=1
MM1 pKa = 7.38PRR3 pKa = 11.84STKK6 pKa = 10.12RR7 pKa = 11.84ATSRR11 pKa = 11.84RR12 pKa = 11.84PKK14 pKa = 10.02RR15 pKa = 11.84FARR18 pKa = 11.84RR19 pKa = 11.84KK20 pKa = 9.63AGRR23 pKa = 11.84KK24 pKa = 9.05SSGSQLVSKK33 pKa = 11.0AMLYY37 pKa = 10.32KK38 pKa = 10.73AIARR42 pKa = 11.84NLEE45 pKa = 3.87VKK47 pKa = 9.1MANIGFNYY55 pKa = 9.29TSQSSTLTSLATNFYY70 pKa = 10.86CPLPPVNLGIGSDD83 pKa = 3.49QRR85 pKa = 11.84IGDD88 pKa = 4.11KK89 pKa = 10.31IRR91 pKa = 11.84PLKK94 pKa = 10.56LVIRR98 pKa = 11.84GYY100 pKa = 10.28ISLVGDD106 pKa = 3.49VDD108 pKa = 3.96SSSRR112 pKa = 11.84ALAGRR117 pKa = 11.84LFCGLDD123 pKa = 3.19KK124 pKa = 11.21AILSKK129 pKa = 10.15TDD131 pKa = 3.82FSSLNINILDD141 pKa = 3.83NGGTSTTYY149 pKa = 10.69GGNLFGQCALKK160 pKa = 8.74NTQMYY165 pKa = 9.52RR166 pKa = 11.84WYY168 pKa = 10.5ADD170 pKa = 2.92KK171 pKa = 11.11KK172 pKa = 10.79FFLTKK177 pKa = 9.03PTGIYY182 pKa = 9.87QSAAIGGGVSSTPRR196 pKa = 11.84AQDD199 pKa = 3.3LFRR202 pKa = 11.84EE203 pKa = 4.42FTITLTKK210 pKa = 10.31KK211 pKa = 9.92HH212 pKa = 6.18LPAQFFYY219 pKa = 11.17DD220 pKa = 3.49STEE223 pKa = 3.89NATFPVNFCPFIALGYY239 pKa = 8.42CDD241 pKa = 4.96PMTSGIAGNGFMGMTYY257 pKa = 9.48TAVLHH262 pKa = 5.36YY263 pKa = 9.92TDD265 pKa = 3.93AA266 pKa = 5.89

Molecular weight: 29.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

557

266

291

278.5

31.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.181 ± 0.738

2.334 ± 0.308

5.745 ± 0.835

4.129 ± 2.032

4.309 ± 1.155

7.181 ± 1.247

2.154 ± 0.95

5.925 ± 0.193

7.361 ± 0.657

8.797 ± 0.153

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.334 ± 0.202

3.95 ± 0.635

3.052 ± 0.988

3.232 ± 0.152

5.925 ± 0.316

7.002 ± 1.368

9.695 ± 0.71

3.591 ± 0.14

1.077 ± 0.475

5.027 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski