Mycobacterium phage TipsytheTRex

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GK54|A0A2D1GK54_9CAUD Minor tail protein OS=Mycobacterium phage TipsytheTRex OX=2041553 GN=SEA_TIPSYTHETREX_27 PE=4 SV=1
MM1 pKa = 7.93SFQLWMANLDD11 pKa = 3.44AYY13 pKa = 9.42MQEE16 pKa = 4.08TFGVGRR22 pKa = 11.84GDD24 pKa = 3.41IADD27 pKa = 3.33WTYY30 pKa = 11.56RR31 pKa = 11.84DD32 pKa = 4.34AYY34 pKa = 10.96DD35 pKa = 4.69DD36 pKa = 3.96GLTFRR41 pKa = 11.84EE42 pKa = 4.43AAHH45 pKa = 5.47QAINYY50 pKa = 7.34EE51 pKa = 4.37FAA53 pKa = 5.11

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GKI7|A0A2D1GKI7_9CAUD HNH endonuclease OS=Mycobacterium phage TipsytheTRex OX=2041553 GN=SEA_TIPSYTHETREX_4 PE=4 SV=1
MM1 pKa = 7.25SWAGSGRR8 pKa = 11.84RR9 pKa = 11.84QEE11 pKa = 5.38LPEE14 pKa = 4.38DD15 pKa = 3.29WEE17 pKa = 4.35LNYY20 pKa = 10.43RR21 pKa = 11.84LPVLSRR27 pKa = 11.84DD28 pKa = 3.11RR29 pKa = 11.84WLCQINGPGCVRR41 pKa = 11.84AATDD45 pKa = 2.9VDD47 pKa = 4.26HH48 pKa = 7.46IKK50 pKa = 10.61RR51 pKa = 11.84GNDD54 pKa = 3.12HH55 pKa = 6.26SHH57 pKa = 6.89SNLQSLCWVCHH68 pKa = 4.91GKK70 pKa = 10.31KK71 pKa = 10.28SSAEE75 pKa = 3.71GVARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.69LRR84 pKa = 11.84ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.79RR89 pKa = 11.84PPEE92 pKa = 3.6RR93 pKa = 11.84HH94 pKa = 5.7PGRR97 pKa = 11.84RR98 pKa = 3.38

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16235

38

838

174.6

19.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.707 ± 0.328

0.961 ± 0.114

6.326 ± 0.231

7.059 ± 0.332

3.314 ± 0.165

8.445 ± 0.522

2.008 ± 0.17

5.069 ± 0.143

4.823 ± 0.245

8.075 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.146

3.258 ± 0.192

5.322 ± 0.236

3.517 ± 0.191

6.535 ± 0.363

5.18 ± 0.188

5.932 ± 0.245

7.047 ± 0.229

1.94 ± 0.128

3.018 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski