Caviibacterium pharyngocola
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M8RTR8|A0A2M8RTR8_9PAST 30S ribosomal protein S5 OS=Caviibacterium pharyngocola OX=28159 GN=rpsE PE=3 SV=1
MM1 pKa = 7.65 AIVGLFYY8 pKa = 11.01 GSDD11 pKa = 3.18 TGNTEE16 pKa = 3.78 NVAKK20 pKa = 10.15 MIQKK24 pKa = 9.57 QLGAEE29 pKa = 4.06 LVDD32 pKa = 3.37 IRR34 pKa = 11.84 DD35 pKa = 3.49 IAKK38 pKa = 8.47 STKK41 pKa = 9.86 EE42 pKa = 4.18 DD43 pKa = 3.05 IEE45 pKa = 5.8 AYY47 pKa = 10.09 DD48 pKa = 3.9 FLMFGIPTWYY58 pKa = 10.25 YY59 pKa = 11.58 GEE61 pKa = 4.69 AQCDD65 pKa = 3.26 WDD67 pKa = 5.24 DD68 pKa = 4.13 FFPTLEE74 pKa = 4.64 QIDD77 pKa = 4.09 FTDD80 pKa = 3.2 KK81 pKa = 10.68 LVAIFGCGDD90 pKa = 3.25 QEE92 pKa = 5.04 DD93 pKa = 4.3 YY94 pKa = 11.79 AEE96 pKa = 4.32 YY97 pKa = 10.5 FCDD100 pKa = 4.34 AMGTVRR106 pKa = 11.84 NIIEE110 pKa = 4.26 PKK112 pKa = 9.82 GAIIVGNWSTEE123 pKa = 3.52 GYY125 pKa = 8.71 TFEE128 pKa = 4.7 SSQALVDD135 pKa = 3.73 EE136 pKa = 4.44 NTFVGLCIDD145 pKa = 4.19 EE146 pKa = 4.82 DD147 pKa = 3.98 RR148 pKa = 11.84 QPEE151 pKa = 4.27 LTGEE155 pKa = 4.25 RR156 pKa = 11.84 VEE158 pKa = 4.37 KK159 pKa = 8.58 WTKK162 pKa = 8.92 QVYY165 pKa = 10.69 DD166 pKa = 4.11 EE167 pKa = 4.44 MCLAEE172 pKa = 4.39 LAA174 pKa = 4.24
Molecular weight: 19.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.643
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.554
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.126
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|A0A2M8RYH0|A0A2M8RYH0_9PAST Uncharacterized protein OS=Caviibacterium pharyngocola OX=28159 GN=CVP04_02260 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2334
0
2334
729501
30
2655
312.6
34.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.84 ± 0.071
1.093 ± 0.019
5.11 ± 0.034
6.245 ± 0.046
4.409 ± 0.039
6.736 ± 0.053
1.989 ± 0.025
6.873 ± 0.046
5.903 ± 0.042
10.588 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.317 ± 0.026
4.736 ± 0.042
3.734 ± 0.034
4.849 ± 0.044
4.711 ± 0.039
5.666 ± 0.037
5.269 ± 0.042
6.569 ± 0.045
1.157 ± 0.021
3.206 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here