Pseudomonas phage TC6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Paundecimvirus; unclassified Paundecimvirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BQE0|A0A2H5BQE0_9CAUD DNA polymerase I OS=Pseudomonas phage TC6 OX=2060947 GN=TC6_040 PE=3 SV=1
MM1 pKa = 7.45VIYY4 pKa = 9.55VWPDD8 pKa = 3.46GYY10 pKa = 10.25WLYY13 pKa = 11.22EE14 pKa = 3.88WAVEE18 pKa = 4.46DD19 pKa = 3.86NASLARR25 pKa = 11.84LASPHH30 pKa = 6.46KK31 pKa = 10.82KK32 pKa = 9.22IDD34 pKa = 3.73LDD36 pKa = 3.5NHH38 pKa = 6.72LDD40 pKa = 3.63YY41 pKa = 11.41NLTVDD46 pKa = 5.19EE47 pKa = 4.75INACIEE53 pKa = 4.23ALDD56 pKa = 3.93EE57 pKa = 4.3

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BQG9|A0A2H5BQG9_9CAUD DUF3310 domain-containing protein OS=Pseudomonas phage TC6 OX=2060947 GN=TC6_031 PE=4 SV=1
MM1 pKa = 7.72AFQAKK6 pKa = 7.18NHH8 pKa = 5.8KK9 pKa = 9.23RR10 pKa = 11.84QPDD13 pKa = 3.23TRR15 pKa = 11.84MSNKK19 pKa = 9.99AKK21 pKa = 10.04IARR24 pKa = 11.84RR25 pKa = 11.84EE26 pKa = 4.07DD27 pKa = 3.93KK28 pKa = 10.55IDD30 pKa = 3.56ALNAEE35 pKa = 4.58RR36 pKa = 11.84TPEE39 pKa = 3.52ARR41 pKa = 11.84KK42 pKa = 9.55ARR44 pKa = 11.84KK45 pKa = 9.39AMNDD49 pKa = 2.81VVYY52 pKa = 10.56RR53 pKa = 11.84GSRR56 pKa = 11.84TNLNDD61 pKa = 3.33FWNPKK66 pKa = 8.77GKK68 pKa = 10.11RR69 pKa = 11.84LAKK72 pKa = 10.31GSGVKK77 pKa = 8.63ATKK80 pKa = 10.02INRR83 pKa = 11.84ALKK86 pKa = 10.69AKK88 pKa = 9.75TNN90 pKa = 3.67

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

15654

42

1554

220.5

24.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.33 ± 0.456

1.252 ± 0.188

6.228 ± 0.157

7.008 ± 0.344

3.999 ± 0.184

7.167 ± 0.32

1.942 ± 0.157

5.245 ± 0.172

6.823 ± 0.454

7.71 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.114

5.181 ± 0.224

3.801 ± 0.172

4.05 ± 0.375

5.123 ± 0.191

5.57 ± 0.294

5.289 ± 0.285

7.091 ± 0.221

1.75 ± 0.13

3.852 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski