Gillisia mitskevichiae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gillisia

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495NVP6|A0A495NVP6_9FLAO Antitoxin ParD1/3/4 OS=Gillisia mitskevichiae OX=270921 GN=BC962_3246 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84FLAPYY7 pKa = 10.07YY8 pKa = 10.72SLLFLLLVFLSCDD21 pKa = 2.77SDD23 pKa = 4.43NPVNPPDD30 pKa = 4.36TPDD33 pKa = 3.71SNPDD37 pKa = 3.16GNPVVTTEE45 pKa = 5.02DD46 pKa = 3.24IIEE49 pKa = 4.35IPDD52 pKa = 3.6EE53 pKa = 4.18YY54 pKa = 11.04FKK56 pKa = 11.41NALINTNIIDD66 pKa = 3.94TDD68 pKa = 3.44NDD70 pKa = 3.53EE71 pKa = 5.12QGDD74 pKa = 4.12SDD76 pKa = 4.77IDD78 pKa = 4.06LNNDD82 pKa = 3.23GEE84 pKa = 4.55IQVSEE89 pKa = 4.24AEE91 pKa = 4.68KK92 pKa = 10.27ISGLIFSINYY102 pKa = 9.17AEE104 pKa = 4.22VRR106 pKa = 11.84RR107 pKa = 11.84VIDD110 pKa = 3.37FTGANSFKK118 pKa = 10.45NLKK121 pKa = 8.66VLKK124 pKa = 10.47ISGTSDD130 pKa = 2.84RR131 pKa = 11.84LEE133 pKa = 4.38PNFDD137 pKa = 4.47SIQISYY143 pKa = 10.63DD144 pKa = 3.37FSGLKK149 pKa = 9.63QLEE152 pKa = 4.19KK153 pKa = 10.8LQISGVDD160 pKa = 3.11ASYY163 pKa = 11.08IDD165 pKa = 4.44NINISGLSKK174 pKa = 10.93LKK176 pKa = 10.33IANLDD181 pKa = 3.69GNKK184 pKa = 9.27PRR186 pKa = 11.84YY187 pKa = 9.26FDD189 pKa = 3.55GDD191 pKa = 3.75YY192 pKa = 10.87NIPFNFIIMNMNDD205 pKa = 3.25CTALEE210 pKa = 4.6DD211 pKa = 4.78LSLTNSWLTINYY223 pKa = 8.95CDD225 pKa = 3.57TPAIKK230 pKa = 9.34TLNLFYY236 pKa = 11.18LEE238 pKa = 4.47GGEE241 pKa = 4.41PDD243 pKa = 4.51MLDD246 pKa = 3.41LHH248 pKa = 7.05CLTNLEE254 pKa = 4.18WLDD257 pKa = 3.39ISEE260 pKa = 4.63NSLDD264 pKa = 4.61SLVLKK269 pKa = 10.65NNSVLTYY276 pKa = 10.53LRR278 pKa = 11.84ADD280 pKa = 4.99DD281 pKa = 4.46IGSAYY286 pKa = 9.96EE287 pKa = 3.91NNNYY291 pKa = 9.38PFLDD295 pKa = 3.93YY296 pKa = 11.47LCIDD300 pKa = 4.72DD301 pKa = 4.84FQQEE305 pKa = 3.88VDD307 pKa = 3.72QVSTLVNEE315 pKa = 4.2NTIVTTNCEE324 pKa = 3.9FF325 pKa = 4.05

Molecular weight: 36.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495PRX0|A0A495PRX0_9FLAO UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase OS=Gillisia mitskevichiae OX=270921 GN=BC962_1561 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.05RR21 pKa = 11.84MASVNGRR28 pKa = 11.84KK29 pKa = 9.21VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.62LSVSSEE47 pKa = 4.04TRR49 pKa = 11.84HH50 pKa = 5.02KK51 pKa = 10.46HH52 pKa = 4.65

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3228

0

3228

1068363

29

3291

331.0

37.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.253 ± 0.039

0.699 ± 0.013

5.485 ± 0.037

7.115 ± 0.045

5.269 ± 0.036

6.328 ± 0.047

1.695 ± 0.02

8.287 ± 0.042

7.841 ± 0.055

9.586 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.025

6.119 ± 0.044

3.365 ± 0.025

3.259 ± 0.021

3.438 ± 0.026

6.763 ± 0.033

5.383 ± 0.047

5.978 ± 0.038

1.005 ± 0.013

3.895 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski