Streptococcus phage Javan554

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BFA7|A0A4D6BFA7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan554 OX=2548256 GN=Javan554_0051 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.18KK3 pKa = 10.48LKK5 pKa = 10.69LFGVGFLALAALGFILQALGLAPKK29 pKa = 9.7TEE31 pKa = 4.35VPEE34 pKa = 4.48TPQVTTQTTTIEE46 pKa = 3.89QSSISEE52 pKa = 4.18SSTTEE57 pKa = 3.77TNEE60 pKa = 3.68QSSEE64 pKa = 3.75KK65 pKa = 10.25VAYY68 pKa = 9.85DD69 pKa = 3.48VAEE72 pKa = 4.23MNVKK76 pKa = 8.46ITDD79 pKa = 3.69SFNEE83 pKa = 4.25SVQFNQDD90 pKa = 2.55GHH92 pKa = 7.6DD93 pKa = 3.88GYY95 pKa = 11.31EE96 pKa = 3.87WTSYY100 pKa = 10.27IYY102 pKa = 9.94EE103 pKa = 4.5IKK105 pKa = 10.87LKK107 pKa = 11.1DD108 pKa = 3.44NGAINATVNDD118 pKa = 3.93NFSTLSDD125 pKa = 3.7TEE127 pKa = 4.02KK128 pKa = 10.58TEE130 pKa = 3.85VLNSVSRR137 pKa = 11.84AVNMVVFLEE146 pKa = 4.24TEE148 pKa = 3.7EE149 pKa = 4.34DD150 pKa = 3.0KK151 pKa = 11.44SYY153 pKa = 11.18FITAYY158 pKa = 10.08DD159 pKa = 3.42QAGNKK164 pKa = 8.15VAQSHH169 pKa = 4.64MTNVLEE175 pKa = 4.42YY176 pKa = 10.8NFEE179 pKa = 4.09

Molecular weight: 19.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BF81|A0A4D6BF81_9CAUD Uncharacterized protein OS=Streptococcus phage Javan554 OX=2548256 GN=Javan554_0045 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.01RR4 pKa = 11.84IKK6 pKa = 10.28KK7 pKa = 10.1KK8 pKa = 10.48YY9 pKa = 7.88EE10 pKa = 3.45LEE12 pKa = 3.81NRR14 pKa = 11.84IRR16 pKa = 11.84TLEE19 pKa = 3.82THH21 pKa = 6.75IDD23 pKa = 3.68FLIDD27 pKa = 4.27QNNQLWNIVEE37 pKa = 4.45RR38 pKa = 11.84NAQATNARR46 pKa = 11.84FDD48 pKa = 4.12KK49 pKa = 10.85IEE51 pKa = 4.02KK52 pKa = 9.79QVANGNTKK60 pKa = 10.1KK61 pKa = 10.54PFWKK65 pKa = 10.34RR66 pKa = 3.03

Molecular weight: 8.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11485

50

1124

220.9

24.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.314 ± 0.76

0.47 ± 0.079

6.26 ± 0.354

7.027 ± 0.566

4.354 ± 0.256

6.844 ± 0.505

1.454 ± 0.161

6.861 ± 0.274

7.61 ± 0.463

7.706 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.144

5.633 ± 0.264

3.013 ± 0.156

3.997 ± 0.225

4.536 ± 0.317

6.574 ± 0.427

6.199 ± 0.42

6.922 ± 0.236

1.245 ± 0.147

3.674 ± 0.335
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski